The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effect of Smilax china L. Rhizome Extract on Heavy Metal Contents in Rats

청미래 덩굴 뿌리 추출물 투여가 실험동물의 체내 중금속 함량에 미치는 영향

  • Received : 2011.05.17
  • Accepted : 2011.06.26
  • Published : 2011.06.30
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Abstract

Smilax china L. rhizome extract(SCE), called 'Tobokreung' in Korean traditional medicine was investigated the influence on heavy metal contents(particularly Pb, Cd, As, and Hg) in Sprague-Dawley rats for 3 weeks. The test groups were divided into 4 group, normal, control, and SCE feeding groups, SC1(13 mg/kg) and SC2(26 mg/kg), respectively. The three group except normal group, were fed heavy metal such as Pb, Cd, As, and Hg. Body weight gain and the weight of target organs (liver and kidney) were determined and had not shown significant differences. Pb, Cd, and As contents in the kidney of SCE feeding groups were tended to decrease after 3 weeks, and SC2 group showed remarkably decrease of them. In the liver, the 3 heavy metal contents except Cd of SC2 group, were decreased rather than that of control group. Pb contents in the serum and the hair of the SC2 group showed significantly decreasing. All the taken together, we investigated the effect of SCE on the 4 heavy metal contents in rats for 3 weeks, and found out that more dosage of SCE made lower heavy metal contents in vivo, for the first time.

한방에서 토복령으로 알려진 청미래 덩굴 뿌리(Smilax china L. rhizome) 추출물 투여가 체내 중금속 함량에 미치는 영향을 알아보고자 4주령 SD rat을 대상으로 중금속을 투여하지 않은 정상군, 중금속(Pb, Cd, As, Hg) 투여한 대조군 및 청미래 덩굴 뿌리 추출물(SCE)을 체중 kg당 13 mg(SC1)과 26 mg(SC2)씩 투여한 군으로 나누어 3주간 사육하였다. 체중 증가나 표적 장기의 무게는 실험군들 간의 유의적인 차이가 없는 것으로 나타났다. 신장에서의 중금속 축적량은 대조군에 비하여 SCE 투여군에서 3주 후 농도가 감소하는 경향을 보였고, 특히 SC2군에서 유의성 있는 감소를 보였다. 간에서는 대조군과 비교해 SCE 투여군에서 Cd을 제외하고 3주 후 농도가 감소하는 경향을 보였다. 혈청의 경우 SC2군에서 Pb와 Cd의 유의적인 감소가 나타났다. 털에서 중금속 함량을 비교한 결과, SC2군에서 Pb의 함량이 현저하게 감소한 것으로 나타났다. 본 연구에서 체내의 중금속 축적 장기인 간과 신장 그리고 혈액 및 털에서 Pb와 Cd, As, Hg의 함량을 측정하였으며, SCE 투여량이 증가할수록 그 함량이 감소하였다.

Keywords

References

  1. 여정원. 2004. 오염된 퇴적물에 노출된 숭어, Mugil cephalus 의 체내 중금속 축적. 인하대학교 대학원 석사학위논문, pp.19-37
  2. 최진규. 2001. 약이 되는 우리 풀, 꽃, 나무. pp.113-125. 한문화
  3. AOAC. 1990. Official Method of Analysis. 15th ed., Association of Official Analytical Chemists, p237
  4. Choi HY. 2004. Antimicrobial effect of ethanol extract of Smilax china leaf. Korean J Sanitation 19:22-30
  5. Choi SI, Lee JH, Lee SR. 1994. Effect of green tea beverage for the removal of cadmium and lead by animal experiments. Korean J Food Sci Technol 26:745-749
  6. Choi YS. 2008. The 108 Wild Plants for Drug. Hanam company
  7. Corpas I, Antonio MT. 1998. Study of alterations produced by cadmium and cadmium/lead administration during gestational and early lactation periods in the reproductive organs of the rat. Environ Safety 41:180-188 https://doi.org/10.1006/eesa.1998.1690
  8. Flora SJS, Mittal M, Mehta A. 2008. Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian J Med Res 128:501-523
  9. Flora SJS, Pachauri V. 2010. Chelation in metal intoxication. Int J Environ Res Public Health 7:2745-2788 https://doi.org/10.3390/ijerph7072745
  10. Hammond PB, Minnema DJ, Succop PA. 1993. Reversibility of lead-induced depression of growth. Toxicol Appl Pharmacol 123:9-15 https://doi.org/10.1006/taap.1993.1215
  11. Han JH, Lee WJ, Jo SG, Lee MJ, Jeong MR, Chon JW, Kim UY. 2003. Nutritional characteristics and damage mitigation effects on heavy-metals exposure of Peking-duck by-product extracts added with medicinal herbs (II) damage mitigation effects on heavy-metals exposure of peking-duck by-product extracts. J East Asian Soc Dietary Life 13:293-304
  12. Kim AJ, Shin SM, Jung JS. 2010. Quality characteristics of sulgidduk added with black ginseng extracts. Korean J Food & Nutr 23:386-391
  13. Kim AJ, Yuh CS, Bang IS, Park HY, Lee GS. 2007. An investigation the preparation and physicochemical properties of oddi jelly using mulberry fruit powder. Korean J Food & Nutr 20:27-33
  14. Kim DJ, Cho SY, Kim MJ. 2003. Effect of Korean traditional teas on plasma and hepatic lipid levels in lead-administered rats. J Korean Soc Food Sci Nutr 32:263-268 https://doi.org/10.3746/jkfn.2003.32.2.263
  15. Kim SJ, Baek SH, Heo JW, Kim US, Lee JD, Kang GW, Park SH, Han JH, Jeong SY, Lee SH. 2002. Effect of Cassia tora L. powder added-diets on the accumulation of cadmium in rat. J East Asian Soc Dietary Life 12:554-565
  16. Kim TJ. 2009. Korean Plant Edition. Yearim Company, Seoul
  17. Kim US, Lee CH, Kim SJ, Lee JD, Moon KH, Baek SH. 1995. Effect of the Aloe arboresceus added-diet on the cadmium toxicity in rat. Korean J Food Sci Technol 27:555-563
  18. Kwan OR, Kim MK. 1992. The effect of dietary protein and calcium levels on metallothionein and histopathological changes during cadmium intoxication in rats. Korean J Nutr 25:360-378
  19. Lee HH, Cheong MJ, Huh J, Song SY, Boo HO. 2009. Effects of Momordica charantiaa L. water extracts on the rat liver and kidney with acute toxicated by lead. Korean J Microscopy 39:355-363
  20. Li YL, Gan GP, Zhang HZ, Wu HZ, Li CL, Huang YP, Liu YW, Liu JW. 2007. A flavonoid glycoside isolated from Smilax china L. rhizome in vitro anticancer effects on human cancer cell lines. J Ethnopharm 113:115-124 https://doi.org/10.1016/j.jep.2007.05.016
  21. Oh YL. 2011. Protective effect of Smilax china L. extract on the cytotoxicity induced by chromium of environmental pollutant. J Korean Soc People Plants Environ 14:29-34
  22. Park SH, Shin EH, Park SJ, Han JH. 2005. Effect of Pekingduck by-product extracts supplemented with medicinal herbs on serum heavy metal levels and blood parameters of rats exposed to lead and mercury. J Korean Soc Food Sci Nutr 34:476-483 https://doi.org/10.3746/jkfn.2005.34.4.476
  23. Rho JH, Han CK, Lee LH, Chung YK. 1997. Effect of pork as protein on cadmium toxicity in rats. Korean J Anim Sci 39: 605-616
  24. Sheehan JE, Pitot HC, Kasper CB. 1991. Transcriptional regulation and localization of the tissue-specific induction of epoxide hydrolase by lead acetate in rat kidney. J Biol Chem 266: 5122-5127
  25. Song HS, Park YH, Jung SH, Kim DP, Jung YH, Lee MK, Moon KY. 2006. Antioxidant activity of extracts from Smilax china root. J Korean Soc Food Sci Nutr 35:1133-1138 https://doi.org/10.3746/jkfn.2006.35.9.1133
  26. Suzuki T, Yoshida A. 1979. Effect of dietary supplementation of iron and ascorbic acid on lead toxicity in rats. J Nutr 109:982-989
  27. Widmaier EP, Raff H, Strang KT. 2004. Defence mechanisms of the body. Vander, Sherman, Luciano's human physiology. The Mechanisms of Body Function. 9th ed. pp 736-737. Mcgraw-Hill

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