The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of a Corn Extract on Mouse Splenocyte and Cytokine Production by Peritoneal Macrophages

4주 동안의 옥수수 추출물 투여가 마우스 비장세포와 대식세포 생성에 미치는 영향

  • Ryu, Hye-Sook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
  • 류혜숙 (상지대학교 보건과학대학 식품영양학과)
  • Received : 2011.01.24
  • Accepted : 2011.03.28
  • Published : 2011.03.30
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Abstract

This study was performed to investigate the in vitro effect of a corn water extract on immune function. Splenocyte proliferation was determined by the MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl terazolium bromide) assay after preparing asingle cell suspension. Production of macrophage-secreted interleukin(IL)-$1{\beta}$, IL-6, and interferon(IFN)-${\gamma}$, was detected by ELISA using a cytokine assay kit. After a 48-hr incubation with mitogens(ConA or lipopolysaccharide), mice splenocyte proliferation increased with the addition of a corn water extract supplement at 10, 50, 100, 250, 500, or $1,000\;{\mu}g/m\ell$. Production of IL-$1{\beta}$, IL-6, and IFN-${\gamma}$ increased in treatments supplemented with the corn water extract. In an in vitro study, splenocyte proliferation increased when $50\sim1,000\;{\mu}\ell/m\ell$ corn water extract was added. In an ex vivo experiment, the highest production of cytokines by activated peritoneal macrophages was observed in mice orally administered 500 mg/kg body weight/day.

Keywords

References

  1. Ahn HK, Kil HB, Yoo HE, Oh DH. 1990. Effect of lipid contentd on the physicochemical of acorn starch. J Korean Agric Chem Soc 33:293-300
  2. Barnes PJ, Liew FY. 1995. Nitric oxide and asthmatic inflammation. Immunology Today 16:128-130 https://doi.org/10.1016/0167-5699(95)80128-6
  3. Cho SH, Yang KM, Bae BS, In SA, Yu RN. 1988. Effect of sea tangle intake on cytokine production in macrophage from normal and diabetic mice. J Food Sci Nutr 27:952-959
  4. Hyun YH, Nam H, Pyun JW. 2008. Quality characteristics of Sulgidduk with prepared glutinous corn flour. Korean J Food & Nutr 21:293-299
  5. Joung YH. 2003. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni. Seoul. Korea
  6. Kim HP, Son KH, Kang SS. 2004. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacological Sci 96:229-245 https://doi.org/10.1254/jphs.CRJ04003X
  7. Kim YI. 2001. The quality characteristics of rice-corn cakes. Korean J Food Cookery Sci 5:426-430
  8. Lee HM, Chang UJ. 2001. Effect of corn peptide on the lipid metabolism in rat. Korean J Dietary Culture 16:416-422
  9. Lee HS, Ju DN, Kim BL, Kim SH. 2009. Effect of dietary intake of ultra fine or nano scale pulverized cornstarch on the growing performance and gut function in rat. Korean J Nutr 42:740-749 https://doi.org/10.4163/kjn.2009.42.8.740
  10. Lee JH. 2002. Immunostimulative effect of hot-water extracts from Codonopsis lanceolata on lymphocyte and clonal macrophage. Korean J Food Sci Technol 34:732-736
  11. Lim SD, Seong KS, Kim KS, Han DU. 2007. Effects of fermented milk hot water extracts from Acanthopanax senticosus and Codonopsis lanceolata on the immune status of mouse. Korean J Food Sci Technol 39:323-329
  12. Mishell BB, Shiigi SM. 1980. Selected Methods in Cellular Immunology. 1st ed. Sanfrancisco. WH Freeman and Co. 4
  13. Park HA. 2003. Enhancing effect of Ixeris sonchifolia Hance Oenanthe javanica, and Fagopyrum escluentum Moench on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni. Seoul. Korea
  14. Park JS, Chyun JH. 1993. Effects of low fat diet and saturated fat supplementation on the immune status of BALB/c mouse. Korean J Nutr 26:578-585
  15. Pisa P, Halapi E, Pisa EK, Gerdin E, Hising C, Buchi A. 1992. Selective expression of interleukin 10, interferon gamma and granulocyte-macrophage colony-stimulating factor in ovarian cancer biopsies. Proc Nual Acad Sci 89:7708-7712 https://doi.org/10.1073/pnas.89.16.7708
  16. Ryu BH, Kim DS, Cho KJ, Sin DB. 1987. Antitumor activity of seaweeds toward Sarcoma-180. Korean J Food Sci Technol 25:595-596
  17. Ryu HS, Jung YH, Kim HS. 2007. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. Korean J Nutr 40:639-649
  18. Ryu HS, Kim HS. 2005. Effects of Job's tear extracts on mouse immune cell ativation. J Korean Diet Assoc 11:44-50
  19. Ryu HS, Kim J, Kim HS. 2006. Enhancing effect of Sorghum bicolor L. Moench(Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Korean J Food & Nutr 19:176-182
  20. Shan BE, Yoshida Y, Kuroda E, Yamashira U. 1999. Immunomodulating activity of seaweed extract on human lymphocytes in vitro. Int J Immunopharmacol 21:59-70 https://doi.org/10.1016/S0192-0561(98)00063-0
  21. Suh JS. 1996. Effect of Codonopsis lanceolata radix water extract on immunocyte. Korean J Food & Nutr 9:379-384
  22. Sun JS, Eun JS. 1998. Isolation of active components on immunocytes from Codonopsis lanceolatae. Korean J Community Nutrition 31:1076-1081
  23. Sypek JP, Chung CL, Mayer SH, Subramanyam JM, Goldman SJ, Sieburth DS, Wolf SF, Schaub RG. 1994. Ressolution of Cutaneous leishmaniasis: Interleukin 12 initates a protective T helper type 1 immune response. J Exp Med 263:235-237

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