한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제15권1호
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  • Pages.1-7
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  • 2011
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

Expression of Wee1 Gene in the Ascidian, Halocynthia roretzi Embryo

  • Lee, Won-Young (Dept. of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Ham, Hwa-Su (Dept. of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Gil-Jung (Dept. of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 투고 : 2011.01.09
  • 심사 : 2011.03.04
  • 발행 : 2011.03.31
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초록

It is suggested that FGF/Ras/MEK/Erk signaling plays crucial roles in specification and cell division of the mesodermal precursor cells in ascidian embryos. To investigate how the number of cell division in tissue precursor cells is determined, we have characterized Wee1 homolog, Hr-Wee1 of the ascidian Halocynthia roretzi. We found that the Hr-Wee1 mRNA is expressed both maternally and zygotically. Maternal transcript is localized to the cytoplasm in the animal cells, while zygotic expression is seen in cells of the endoderm lineage from 32-cell to 110-cell stages. Zygotic in situ signal is detected in the A-line neural plate cells of neurulae, and in epidermal cells of the head region of tailbud embryos. Embryos treated with MEK signaling inhibitor showed a similar pattern to normal embryos in expression of Hr-Wee1. Therefore, it is likely that MEK signaling does not affect the maternal and zygotic expression of Hr-Wee1.

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참고문헌

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