Plant Biotechnology Reports

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Estimation of nuclear DNA content of various bamboo and rattan species

  • Kumar, Prakash P. (Department of Biological Sciences, National University of Singapore) ;
  • Turner, Ian M. (Department of Biological Sciences, National University of Singapore) ;
  • Rao, A. Nagaraja (Department of Biological Sciences, National University of Singapore) ;
  • Arumuganathan, K. (Flow Cytometry and Imaging Core Laboratory, Benaroya Research Institute at Virginia Mason)
  • Received : 2011.05.11
  • Accepted : 2011.05.23
  • Published : 2011.10.31
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Abstract

We determined the nuclear DNA content (genome size) of over 35 accessions each of bamboo and rattan species from Southeast Asia. The 2C DNA per nucleus was quantified by flow cytometry. The fluorescence of nuclei isolated from the leaves and stained with propidium iodide was measured. The genome size of the bamboo species examined was between 2.5 and 5.9 pg DNA per 2C nucleus. The genome size of the rattan species examined ranged from 1.8 to 10.5 pg DNA per 2C nucleus. This information will be useful for scientists working in diverse areas of plant biology such as biotechnology, biodiversity, genome analysis, plant breeding, physiology and molecular biology. Such data may be utilized to attempt to correlate the genome size with the ploidy status of bamboo species in cases where ploidy status has been reported.

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References

  1. Arumuganathan K, Earle ED (1991a) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208-218 https://doi.org/10.1007/BF02672069
  2. Arumuganathan K, Earle ED (1991b) Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Biol Rep 9:229-241 https://doi.org/10.1007/BF02672073
  3. Bennett MD, Leitch IJ (1995) Nuclear DNA amounts in angiosperms. Ann Bot 76:113-176 https://doi.org/10.1006/anbo.1995.1085
  4. Devi ST, Sharma GJ (1993) Chromosome numbers in some bamboo species of Manipur. BIC Bull 3:16-21
  5. Gui YJ, Wang S, Quan LY, Zhou CP, Long SB, Zheng HJ, Jin L, Zhang XY, Ma NX, Fan LJ (2007) Genome size and sequence composition of moso bamboo: a comparative study. Sci China C Life Sci 50(5):1-6 https://doi.org/10.1007/s11427-007-0016-2
  6. INBAR (2010) Capturing carbon with bamboo: fast and effective in managed stands. International Network for Bamboo and Rattan. http://www.inbar.int/publication/TXT/Brochure%20formatted281%29.pdf
  7. Kumar PP, Raju CR, Chandramohan M, Iyer RD (1985) Induction and maintenance of friable callus from the cellular endosperm of Cocos nucifera L. Plant Sci 40:203-207 https://doi.org/10.1016/0168-9452(85)90204-3
  8. Peng Z, Lu T, Li L, Liu X, Gao Z, Hu T, Yang X, Feng Q, Guan J, Weng Q, Fan D, Zhu C, Lu Y, Han B, Jiang Z (2010) Genome-wide characterization of the biggest grass, bamboo, based on 10,608 putative full-length cDNA sequences. BMC Plant Biol 10:116. doi:10.1186/1471-2229-10-116
  9. van den Engh GJ, Trask BJ, Gray JW (1984) The binding kinetics and interaction of DNA fluorochromes used in the analysis of nuclei and chromosomes by flow cytometry. Histochemistry 84:501-508

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  1. Genetics and genomics of moso bamboo (Phyllostachys edulis): Current status, future challenges, and biotechnological opportunities toward a sustainable bamboo industry vol.9, pp.4, 2011, https://doi.org/10.1002/fes3.229