Asian Journal of Turfgrass Science (아시안잔디학회지)

Turfgrass Society of Korea (한국잔디학회)
권호 표지

Effect of Plant Growth Regulators and Medium Supplements on Plant Regeneration from Stolon of Zoysia matrella Merr

금잔디 포복경으로부터 식물체 재분화에 있어서 식물생장조절물질과 배지첨가물질의 영향

  • Kim, Kyung-Hee (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University) ;
  • Kim, Yong-Goo (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University) ;
  • Heo, Sung-Hyun (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University) ;
  • Bae, Eun-Ji (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Lee, Kwang-Soo (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Park, Nam-Chang (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Lee, Byung-Hyun (Division of Applied Life Science(BK21 program), IALS, Gyeongsang National University)
  • 김경희 (경상대학교 응용생명과학부(BK21) 동물생명과학과, 농업생명과학연구원) ;
  • 김용구 (경상대학교 응용생명과학부(BK21) 동물생명과학과, 농업생명과학연구원) ;
  • 허성현 (경상대학교 응용생명과학부(BK21) 동물생명과학과, 농업생명과학연구원) ;
  • 배은지 (국립산림과학원 남부산림연구소) ;
  • 이광수 (국립산림과학원 남부산림연구소) ;
  • 박남창 (국립산림과학원 남부산림연구소) ;
  • 이병현 (경상대학교 응용생명과학부(BK21) 동물생명과학과, 농업생명과학연구원)
  • Received : 2011.11.07
  • Accepted : 2011.11.30
  • Published : 2011.12.31
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Abstract

To optimize tissue culture conditions for genetic transformation of Zoysia matrella (L.) Merr., we investigated the effects of different plant growth regulators and medium supplements on plant regeneration using stolon explants excised from mature plant grown in a green house. Plant regeneration frequency was 33.3% when stolon tissues with a node were cultured on the regeneration medium supplemented with 0.5 mg/L 2,4-D and 1 mg/L of kinetin. Comparing the basal media tested, MS medium showed higher plant regeneration performance than N6 or SH medium. Addition of 5 mg/L $AgNO_3$ with 10 mg/L cysteine improved frequency of plant regeneration up to 40%. Among different carbon sources, 3% sucrose was found to show the best for regeneration frequency. This rapid and efficient plant regeneration system would be useful for using genetic transformation experiments of manilagrass without intervening callus-mediated regeneration.

금잔디(Zoysia matrella (L.) Merr.)의 포복경 조직으로부터 식물체 재분화를 위한 최적 조직배양조건을 확립하기 위하여 온실에서 재배한 식물체의 포복경 절편체로부터 식물체 재분화에 미치는 몇 가지 요인을 조사하였다. 포복경 조직으로부터 식물체 재분화에 있어서 기본배지로는 MS배지가 N6배지나 SH배지에 비해 높은 효율을 보였으며, 배지에 첨가되는 식물생장조절물질로는 0.5 mg/L 2,4-D와 1 mg/L of kinetin을 첨가했을 때 33.3%로 가장 높은 재분화율을 보였다. 또한 배지에 첨가되는 탄소원 종류로는 sucrose를 3% (v/v) 농도로 첨가해 주었을 때 재분화 효율이 향상되었으며, 재분화배지에 첨가되는 항산화물질로는 5 mg/L $AgNO_3$와 10 mg/L cysteine을 동시에 첨가해 주었을 때 40%의 가장 높은 식물체 재분화 효율을 나타내어 재분화효율이 향상되었다. 본 연구를 통하여 확립한 금잔디의 포복경 재분화 시스템은 캘러스 배양기간 없이 단기간 내에 재분화 식물체를 높은 효율로 얻을 수 있음으로써 유전자 형질전환을 통한 잔디의 분자육종에 있어서 유용하게 사용되어질 수 있을 것이다.

Keywords

References

  1. Al-Khayri, J.M., F.H. Huang, L.F. Thompson, and J.W. King. 1989. Plant regeneration of zoysiagrass from embryo-derived callus. Crop Sci. 29:1324-1325. https://doi.org/10.2135/cropsci1989.0011183X002900050047x
  2. Asano, Y. 1989. Somatic embryogenesis and protoplast culture in Japanese lawngrass (Zoysia japonica). Plant Cell Rep. 8:141-143. https://doi.org/10.1007/BF00716826
  3. Bae, C.H., K. Toyama, S.C. Lee, Y.P. Lim, H.I. Kim, P.S. Song, and H.Y. Lee. 2001. Efficient plant regeneration using mature seed-derived callus in Zoysiagrass (Zoysia japonica Steud.). Kor J Plant Tiss Cult. 28:61-67.
  4. Choi, D.K., G.M. Yang, and J.S. Choi. 2008. Flowering periods, genetic characteristics, and cross-pollination rate of Zoysia spp. in natural open-pollination. Kor. Turfgrass Sci. 22(1):13-24.
  5. Chu, C.C., C.C. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu, and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica 18:659-668.
  6. Davis, R.R. 1956. Question and answers on future of meyer zoysiagrass. Ohio Fm. Home Res. 14:44-45.
  7. Eapan, S and L. Goeorge. 1997. Plant regeneration from peduncle segments of oil seed Brassica-species: Infulence of silver nitrate and silver thio sulfate. Plant Cell Tiss. Org. Cult. 51:229-232. https://doi.org/10.1023/A:1005926108586
  8. Enriquez-Obregon, G.A., D.L. Prieto-Samsonv, G.A. de la Riva and R.I. Vanquez-Padron. 1999. Agrobacterium-mediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment. Plant Cell Tiss. Org, Cult. 59:159-168. https://doi.org/10.1023/A:1006307527449
  9. Fuentes, S.R.L., M.B.P. Calheiros, J. Manetti-Filho, and L.G.E. Vieira. 2000. The effect of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant Cell Tiss. Org. Cult. 60:5-13. https://doi.org/10.1023/A:1006474324652
  10. Ge, Y., T. Norton, and Z.Y. Wang. 2006. Transgenic zoysiagrass (Zoysia japonica) plants obtained by Agrobacterium-mediated transforamation. Plant Cell Rep. 25:792-798. https://doi.org/10.1007/s00299-006-0123-8
  11. Han, J.G., X.Q. Ni, P.S. Mao, X.C. Pu, and G.P. Du. 1996. Method to break dormancy in Zoysia japonica seed (in Chinese). Acta Agrestia Sinca. 4:246-250.
  12. Hwang, O.J. and J.I. Kim. 2009. Recent advances in the development of biotech bentgrass. J Plant Biotechnol. 36:327-335. https://doi.org/10.5010/JPB.2009.36.4.327
  13. Inokuma, C., K. Sugiura, C. Cho, R. Okawara, and S. Kaneko. 1996. Plant regeneration from protoplasts of Japanese lawngrass. Plant Cell Rep. 15:737-741. https://doi.org/10.1007/BF00232218
  14. Inokuma, C., K. Sugiura, N. Imaizumi, and C, Cho. 1998. Transgenic Japanese lawngrass (Zoysia japonica Steud.) plants regenerated from protoplasts. Plant Cell Rep. 17:334-338. https://doi.org/10.1007/s002990050403
  15. Karhu, S.T. 1997. Sugar use in relation to shoot induced by sorbitol and cytokinin in apple. J Am Soc Hort Sci. 122:476-480.
  16. Kim, J.B., S.J. Park, and D.H. Kim. 1997. Callus induction and plant regeneration from stolon in Zoysiagrass. Kor Turfgrass Sci. 11(4):311-320.
  17. Lee, K.W., S.H. Lee, D.G. Lee, H.S. Woo, D.H. Kim, M.S. Choi, K.Y. Kim, H. Lee, and B.H. Lee. 2005. Effect of plant growth regulators and antioxidants on callus induction and plant regeneration from seed culture of orchardgrass. J. Korean Grassl. Sci. 25(3):191-198. https://doi.org/10.5333/KGFS.2005.25.3.191
  18. Lee, K.W., S.H. Lee, D.H. Kim, D.G. Lee, S.H. Won, H.S. Lee, and B.H. Lee. 2006. Effect of callus type and antioxidants on plant regeneration and transformation of tall fescue. J. Korean Grassl. Sci. 26(2):77-82. https://doi.org/10.5333/KGFS.2006.26.2.077
  19. Lee, S.H., B.S. Kim, S.H. Won, J. Jo, K.Y. Kim, G.J. Park, B.R. Sung, H.S. Lee, and B.H. Lee. 2004. Factors affecting callus induction and plant regeneration from mature seed of zoysiagrass (Zoysia japonica Steud.). J Kor Grassl Sci. 24:29-36. https://doi.org/10.5333/KGFS.2004.24.1.029
  20. Murashige, T. and F. Skoog. 1962. A revise medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  21. Noh, H.Y., J.S. Choi, and B.J. Ahn. 1995 Plant regeneration through somatic embryogenesis in zoysiagrass (Zoysia spp.). J Kor Soc Hort Sci. 36:582-587.
  22. Park, G.H. and B.J. Ahn. 1998. Electroporation conditions for DNA transfer into somatic embryogenic cells of Zoysia japonica. Kor J Plant Tissue Culture. 25:13-19.
  23. Park, G.H., J.S. Choi, C.H. Yun, and B.J. Ahn. 1994. DNA delivery into embryogenic cells of zoysiagrass (Zoysia japonica Steud.) and rice (Oryza sativa L.) by electroporation. Kor J Plant Tissue Culture. 21:309-314.
  24. Poeaim, A., Y. Matsuda, and T. Murata. 2005. Plant regeneration from immature inflorescence of zoysiagrass(Zoysia spp.). Plant Biotechnology. 22(3):245-248. https://doi.org/10.5511/plantbiotechnology.22.245
  25. Rim, Y.W., K.Y. Kim, G.J. Choi, Y.C. Lim, and B.Y. Sung. 2001. Callus induction and plant regeneration from seeds of Zoysia japonica Steud. J Kor Grassl Sci. 21:49-52.
  26. Schenk, R.U. and A.C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot. 50:199-204. https://doi.org/10.1139/b72-026
  27. Songst, A.D., D. Duncan, and J. Widholme. 1988. Effect of laminocyclopropane-l-carboxylic acid, silver nitrate, and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Reports. 7:262- 265. https://doi.org/10.1007/BF00272538
  28. Sun, H.J., I.J. Song, T.W. Bae, and H.Y. Lee. 2010. Recent developments in biotechnological improvement of Zoysia japonica Steud. J Plant Biotechnol. 37:400-407. https://doi.org/10.5010/JPB.2010.37.4.400
  29. Toyama, K., C.H. Bae, J.G. Lim, T. Adachi, K.Z. Riu, P.S. Song, and H.Y. Lee. 2003. Production of Herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation. Mol Cells. 16:19-27.
  30. Turgeon, A.J. 1991. Turfgrass management(3th ed.). Prentice-Hall, Englewood Cliffs, New Jersey. p.77-95,139,152-154.
  31. Vikkrant, A. and A. Rashid. 2002. Somatic embryogenesis from immature and mature embryos of a minor millet Paspalum scrobiculatum L. Plant Cell Tissue Organ Cult. 69:71-77. https://doi.org/10.1023/A:1015048529095
  32. Watson, J.R., H.E. Kaerwer, and D.P, Martin. 1992. The turfgrass industry (Chapter 2). In: D.V. Waddington, R.N. Carrow and R.C. Sherman(eds.), Turfgrass. ASA, CSSA and SSSA Madison, WI, USA. p.30-89.
  33. Zhang, L., D. Wu, L. Zhang, and C. Yang. 2007. Agrobacterium-mediated transformation of Japanese lawngrass (Zoysia japonica Steud.) containing a synthetic cryIA(b) gene from Bacillus thuringiensis. Plant Breeding. 126:428-432. https://doi.org/10.1111/j.1439-0523.2007.01320.x