Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
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Simultaneous determination and acute toxicity study of Fructus mume extracts in ICR mice

오매(烏梅)의 다성분동시분석 및 마우스를 이용한 경구 단회투여 급성독성시험

  • Lee, In-Sun (TKM Integrated Research Division, Korea Institute of Oriental Medicine) ;
  • Han, Chang-Hyun (TKM Information Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Chul (College of Pharmacy, Chungbuk National University) ;
  • Hwang, Bang-Yeon (College of Pharmacy, Chungbuk National University) ;
  • Jung, Sang-Hyeok (TKM Integrated Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Young-Joon (Department of Preventive Medicine, College of Oriental Medicine, Daeggu Haany University) ;
  • Jeon, Won-Kyung (TKM Integrated Research Division, Korea Institute of Oriental Medicine)
  • 이인선 (한국한의학연구원 한의융합연구본부) ;
  • 한창현 (한국한의학연구원 전통의학정보연구본부) ;
  • 이철 (충북대학교 약학대학) ;
  • 황방연 (충북대학교 약학대학) ;
  • 정상혁 (한국한의학연구원 한의융합연구본부) ;
  • 이영준 (대구한의대학교 한의과대학 예방의학교실) ;
  • 전원경 (한국한의학연구원 한의융합연구본부)
  • Received : 2011.03.14
  • Accepted : 2011.04.26
  • Published : 2011.04.30
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Abstract

Objectives : Previous studies have shown that Fructus mume (FM) has anti-platelet effects. The present study was performed to determine the acute oral toxicity and quality control of a crude extract of FM in ICR mice. Methods : We investigated the in vivo single dose acute toxicity of FM 95% ethanol extract. This test was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000mg/kg body weight, respectively. Mortalities, clinical findings, autopsy findings and body weight changes were monitored daily for the 14 days following the administration. HPLC analysis was performed for the simultaneous determination of ursolic acid and p-hydroxycinnamic acid in FM. Reverse-phase chromatography using a C18 column and photodiode array detection at 211 nm was used for quantification of the two maker components. The mobile phase for gradient elution consists of water and acetonitrile. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. The mice did not die after single oral administration of maximum dose of FM. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of FM for ICR mice was more than 5000mg/kg on oral route. The HPLC analysis showed that ursolic acid and p-hydroxycinnamic acid amounts to 9.75- and 0.12% in the extract with the retention times of 47.99- and 15.38 minutes, respectively. Conclusions : These results suggest that no toxic dose level of FM in mice is considered to be more than 5000mg/kg. Consequently, it was concluded that FM have no effect on acute toxicity and side effect in ICR mice. For the quality control of FM extract, simultaneous determination of ursolic acid and p-hydroxycinnamic acid was established.

Keywords

References

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