Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Antibacterial Activity against Pathogens of Acne and Tyrosinase Inhibitory Activity of Extract and Fractions from Bark of Prunus sargentii

산벚나무 수피 추출물 및 용매 분획물의 여드름 원인균에 대한 항균활성과 Tyrosinase 저해 활성

  • Lee, Kyoung-In (Biotechnology Industrialization Center, Dongshin University) ;
  • Yang, Sun-Ah (Dept. of Oriental Medicine Materials, Dongshin University) ;
  • Pyo, Byoung-Sik (Dept. of Oriental Medicine Materials, Dongshin University) ;
  • Kim, Sun-Min (Dept. of Oriental Medicine Materials, Dongshin University)
  • 이경인 (동신대학교 생물자원산업화지원센터) ;
  • 양선아 (동신대학교 한약재산업학과) ;
  • 표병식 (동신대학교 한약재산업학과) ;
  • 김선민 (동신대학교 한약재산업학과)
  • Received : 2011.02.21
  • Accepted : 2011.05.23
  • Published : 2011.06.30
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Abstract

In this study, we investigated on antioxidative activity, antibacterial activity against pathogens of acne and tyrosinase inhibitory activity in 75% EtOH extract and its fractions from bark of Prunus sargen tii. The total polyphenol and flavonoidcontent of the EtOAc fraction were found to be 378.0 mg/g and 67.5 mg/g as the highest content. In the measurement of DPPH radical scavenging ability, EtOAc and BuOH fraction were exhibited stronger scavenging ability than the other fractions and 75% EtOH extract. In antibacterial activity by disc diffusion assay against pathogen of acne, antibacterial activity of the EtOAc fraction and 75% EtOH extract was stronger than the other fractions. Especially, the EtOAc fraction was the highest effective fraction in the antibacterial activity. Moreover, tyrosinase inhibitory activity of EtOAc fraction, BuOH fraction and 75% EtOH extract was higher than the other fractions. In particular, tyrosinase inhibitory activity of the EtOAc fraction showed higher activity than ascorbic acid used as positive control.

Keywords

References

  1. 신민교 (2006) 臨床本草學, 399-400, 도서출판 영림사, 서울.
  2. Kam, W. S. (1981) Pharmaceutical botany. 305-306, National Chinese Medicine Institute.
  3. Park, E. S., Shin, M. K. and Song, H. J. (1998) A study on the antiallergic effect of Cortex Betula Platyphyllae or Cortex Pruni Serrulatae extract. Kor. J. Herbology 13: 57-68.
  4. Lee, H. J., Lee, S. S., Choi, D. H. and Atsushi, K. (2001) Studies on biological activity of wood extractives(VI)-Flavonoids in heartwood of Prunus sargentii. Mokchae Konghak 29: 133-139.
  5. An, B. J., Cho, Y. J., Son, J. H., Park, J. M., Lee, J. Y. and Park, T. S. (2006) Antioxidant effects and tyrosinase inhibition activity of extract of Prunus sargentii Rehder. J. Korean Soc. Appl. Biol. Chem. 49: 145-148.
  6. Lee. H. J., Lee, S. S. and Choi, D. H. (2003) Studies on biological activity of wood extractives(VII)-Antimicrobial and anti-oxidative activities of extractives from the heartwood of Prunus sargentii. Mokchae Konghak 31: 16-23.
  7. Han, B. H. and Han, Y. N. (1978) Immunosuppressant activity of cherry bark extract. Kor. J. Pharmacogn. 9: 173-175.
  8. Park, J. M., Lee, J. Y., Park, T. S., Park, G. H., Park, K. S., Kim, T. H., Cho, Y. J., Kwon, O. J., Choi, K. I. and An, B. J. (2008) Biological activity investigation, and phenol compounds isolation from barks of Prunus sargentii R. Korean J. Medicinal Crop Sci. 16: 173-182.
  9. Kang, G. J. (2007) Inhibitory effect of organic extracts from Prunus yedoensis Matsum barks on the atopic dermatitis-like inflammation. Master's Thesis in Department of Medicine Graduate School Cheju National University, 56-60.
  10. Lim, B. O., Park, D. K., Shin, H. M. and Kim, S. H. (2000) Effects of BuOH fractions isolated from Scutellaria baicalensis, Euginia aromaticz, Sorbus commixta, Betula mandshrica on antibody productivity, metabolism of unsaturated fatty acid and lipid hydroperoxides. Korean J. Oriental Medical Pathology 14: 171-182.
  11. Burton, J, L, and Shuster, S. (1971) The relationship between seborrhea and acne vulgaris. Br. J. Dermatol. 84: 600-604.
  12. Cunliffe, W. J., Holland, D. B. and Jeremy, A. (2004) Come-done formation: etiology, clinical presentation, and treatment. Clin. Dermatol. 22: 367-374. https://doi.org/10.1016/j.clindermatol.2004.03.011
  13. Ahn, Y. G., Kim, S. K., Shin, C. S. and Min, J. H. (2002) Inhibitory effects of wax gourd extract on melanin formation and acne-forming bacterial growth. Kor. J. Food Nutr. 15: 137-143.
  14. Ki, H. G., Yun, S. J., Lee, J. B., Kim, S. J., Lee, S. C. and Won, Y. H. (2005) Microorganism isolated from acne and their antibiotic susceptibility. Kor. J. Dermatol. 43: 871-875.
  15. Lim, Y. S., Myung, K. B., Chung, N. E. and Chung, W. S. (1995) A study on the MIC of antibiotics for Propionibacterium acnes in patients with acne. Kor. J. Dermatol. 33: 437- 444.
  16. Sohn, H. Y., Kim, Y. S., Kum, E. J., Kwon, Y. S. and Son, K. H. (2006) Screening of anti-acne activity of natural products against Propionibacterium acnes. Kor. J. Microbiol. Biotechnol. 34: 265-272.
  17. Kim, N. R., Lim, Y. H., Park, S. W. and Nam, E. S. (2009) Antimicrobial activities of the anti-acne compounds from natural sources. Kor. J. Microbiol. Biotechnol. 37: 80-84.
  18. Yang, H. J., Kim, E. H., Kang, S. T. and Park, S. N. (2009) Antibacterial activity of Platycarya strobilacea extract and stability of the extract-containing cream. Kor. J. Microbiol. Biotechnol. 37: 170-175.
  19. Jung, S. W., Lee, N. K., Kim, S. J. and Han, D. S. (1995) Screening of tyrosinase inhibitor from plants. Kor. J. Food Sci. Technol. 27: 891-896.
  20. Blois, M. S. (1958) Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  21. Otto, F. and Denis, W. (1912) On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biological Chemistry 12: 239-243.
  22. Moreno, M. I. N, Isla, M. I. N., Sampietro, A. R. and Vattuone, M. A. (2000) Comparison of the free radical-scavenging activity of propolis from several region of Argentina. J. Enthropharmacology 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  23. Oh, Y. J., Seo, H. R., Choi, T. M. and Jung, D. S. (2010) Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean J. Medicinal Crop Sci. 18: 373-378.
  24. Yin, Y., Heo, S. I., Jung, M. J. and Wang, M. H. (2009) Antioxidant and antidiabetic effects of various sections of Astragalus membranaceus. Kor. J. Pharmacogn. 40: 1-5.
  25. Eom, H. J., Kim, S. M., Pyo, B. S. and Lee, K. I. (2009) Changes of physiological activity by drying temperature in leaf of Eriobotrya japonica. Kor. J. Pharmacogn. 40: 178- 183.
  26. Park, J. M., Lee, J. Y., Park, T. S., Hyun, S. J., Kim, H. H., Cho, Y. J., Kwon, O. J., Son, A. R., Kim, D. S. and An, B. J. (2008) A study on the cosmeceutical activities of Prunus sargentii R. J. Korean Soc. Appl. Biol. Chem. 51: 70-78.