Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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The transfer of diacylglycerol from lipophor in to fat body in larval Manduca sexta

유충 Manduca sexta 리포포린에 의한 지방체로의 디아실글리세리드 운반

  • Yun, Hwa-Kyung (Department of Biological Sciences, Hanseo University)
  • 윤화경 (한서대학교 생명과학과)
  • Received : 2011.02.24
  • Accepted : 2011.04.07
  • Published : 2011.04.30
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Abstract

This paper was to characterize the transfer of diacylglycerol(DAG) from lipophorin to Manduca sexta larval fat bodies. $[^3H]$-DAG-labeled Lp($[^3H]$-DAG-Lp) was incubated with the larval fat bodies under different times and the time of DAG transfer was determined. Incubation of fat bodies with $[^3H]$-DAG-Lp resulted in accumulation of DAG and TAG in the tissue. The transfer of $[^3H]$-DAG was inhibited in the presence of suramin and unlabeled lipophorin, which would be consistent with a lipophorin receptor. The effects of suramin may be complex because it can change membrane properties when bound to the lipophorin receptor and affect the rate of DAG transfer. To investigate the lipid uptake via receptor-mediated endocytosis, we treated with endocytosis inhibitors, ammonium chloride and chloroquine. The results show that the transfer process of lipid by lipophorin and fat bodies is receptor-mediated endocytosis.

본 논문은 리포포린에 의해 디아실글리세리드(DAG)가 Manduca sexta 유충 지방체로 운반되는 과정을 조사하였다. $[^3H]$-DAG 표지 리포포린($[^3H]$-DAG-Lp)을 시간별로 유충 지방체와 배양하여 지방체로 운반되는 DAG의 방사능을 결정하였다. $[^3H]$-DAG-Lp와 지방체를 배양하면 지방체에 DAG가 축적되며 이의 일부는 지방체에서 트리아실글리세리드(TAG)로 전환되는 것을 알 수 있었다. Suramin과 표지되지 않은 리포포린(unlabeled Lp)의 존재 하에서는 지방체로 운반되는 DAG가 억제되는 데, 이는 DAG를 운반하는 데 리포포린 수용체가 관여한다는 사실을 알 수 있었다. Suramin의 효과는 다소 복잡하지만 리포포린 수용체에 결합하여 막의 특성을 변화시켜 DAG 운반속도에 영향을 주는 것 같다. 지질 운반과정이 수용체-매개 내포작용이라는 사실을 조사하기 위하여 내포작용 억제자인 ammonium chloride와 chloroquine을 처리하였다. 그 결과 리포포린과 지방체사이에서의 지질 운반 기작이 수용체-매개 과정이라는 사실을 보여준다.

Keywords

References

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