Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Lignan contents in Acanthopanax senticosus by HPLC

고속액체크로마토그래피를 이용한 가시오갈피의 리그난 함량

  • Kim, Hye-Min (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Seon-Haeng (Gongju National University of Education) ;
  • Kook, Soon-Ja (Gimpo Agricultural Technology Extension Center) ;
  • Lee, Sang-Hyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2011.07.04
  • Accepted : 2011.09.19
  • Published : 2011.09.30
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Abstract

A reverse-phase system of HPLC using a linear gradient of acetonitrile and deionized water was developed for the quantification lignans, eleutherosides B and E, in Acanthopanax senticosus. The HPLC system consisted of linear gradient of acetonitrile and deionized water, and UV/VIS detection was set at 210 nm. Both eleutherosides B and E contents in different parts of A. senticosus were determined. As a result, the contents of eleutherosides B and E were measured in the leaves (trace amounts and 0.029 mg/g, respectively), stems (0.107 and 1.015 mg/g, respectively), roots (0.026 and 0.390 mg/g, respectively), and fruits (0.022 and 0.043 mg/g, respectively). Moreover, eleutherosides B and E in the water extract were found 0.011 and 0.171 mg/g, respectively.

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References

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