농업과학연구 (Korean Journal of Agricultural Science)

  • 제38권2호
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  • Pages.227-233
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  • 2011
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  • 2466-2402(pISSN)
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  • 2466-2410(eISSN)
충남대학교 농업과학연구소 (Institute of Agricultural Science, CNU)
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작은방울-유리화법에 의한 국화 신초의 초저온동결보존

Cryopreservation of chrysanthemum shoot tips using the droplet-vitrification technique

  • 투고 : 2011.04.19
  • 심사 : 2011.06.20
  • 발행 : 2011.06.30
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초록

This study aimed at developing cryopreservation protocol for chrysanthemum (Dendranthema grandiflora Tzelevcv. peak) shoot apices based on droplet-vitrification procedure, which is a combination of droplet-freezing and solution based vitrification. Progressive preculture of shoot apices in liquid MS medium supplemented with 0.3 and 0.7 M sucrose for 31 and 17 hours, respectively, was found optimum among preculture treatments tested. The composition of both loading and vitrification solutions significantly affected recovery growth of shoot tips before and after cryopreservation. Balancing glycerol and sucrose concentrations in the solutions was beneficial for recovery growth. The highest recovery after cryopreservation was observed when apical shoot tips were extracted from 4-week-old in vitro plantlets, progressively precultured with 0.3-0.5-0.7 M sucrose for 32-16-6 hours, respectively, then treated with loading solution comprising of 1.9 M glycerol + 0.5 M sucrose (35% PVS3 solution). Apices were then dehydrated with the vitrification solution consisted of 50% glycerol + 50% sucrose for 90 minutes then directly immersed in liquid nitrogen.

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참고문헌

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피인용 문헌

  1. Optimization Conditions for Cryopreservation of Deutzia paniculata Nakai, Endangered Plant vol.36, pp.4, 2011, https://doi.org/10.7747/jfes.2020.36.4.274