Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Mapping QTLs for drought tolerance using an introgression line population from a cross between Ilpumbyeo and Moroberekan in rice

벼 일품벼/모로베레칸 조합의 이입계통을 이용한 내건성 유전자 탐지

  • Kang, Ju-Won (Department of Agronomy, Chungnam National University) ;
  • Ju, Hong-Guang (Agricultural Dept., Agricultural college of yanbian Univ.) ;
  • Yang, Paul (Department of Agronomy, Chungnam National University) ;
  • Ahn, Sang-Nag (Department of Agronomy, Chungnam National University)
  • Received : 2011.04.15
  • Accepted : 2011.06.20
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to map quantitative trait loci (QTLs) related to drought stress tolerance. An introgression line population derived from a cross, "Ilpum" / "Moroberekan" was used in this study. $F_1$ plants were backcrossed three times to Ilpum to produce $BC_3F_1$ plants. These plants were advanced by selfing for four generation and a total of 117 $BC_3F_5$ introgression lines were developed. These lines were evaluated for percent seed set and spikelets per panicle under the control (field) and drought condition. To identify QTLs related to drought tolerance, 134 SSR markers showing polymorph isms between the parents were genotyped for the 117 $BC_3F_5$ lines. A total of 6 QTLs associated with drought stress were detected on chromosomes 1, 3, 4, 7 and 10. These include two QTLs for phenotypic acceptability, two QTLs for percent seed set ($R^2$ = 19.0 - 20.9%), and two QTLs for spikelets per panicle ($R^2$ = 22.3 - 23.10%). The Moroberekan alleles at three loci contributed the positive effect for drought tolerance. The SSR markers linked to drought stress tolerance can not only facilitate the selection of valuable genes from Moroberekan, but also allow identification of lines with drought tolerance.

Keywords

References

  1. Causse MA, Fulton TM, Cho YG, Ahn SN, Chunwongse J, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SE et al. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251-1274.
  2. Heu M, Koh HJ. 1991. Newly bred salt tolerant line in rice. Kor. J. Breed. 23: 59-63.
  3. Ju HG, Kim DM, Kang JW, Kim MK, Kim YG, Ahn SN. 2008. Mapping QTLs for agronomic traits using an introgression line population from a cross between Ilpumbyeo and Moroberekan in rice. Korean J. Breed Sci. 40(4): 414-421.
  4. Kim HK, Lee SC, Choi HS, An KS, An GH, Kim SR. 2007. Generation of transgenic rice plants expressing OsDREB1 genes under Ubiquitin or OsPOX1 Promoter. Proc. Annual Symp. Korean Breed. Soc. 39(Supl. 1): 189.
  5. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi- Shinozaki K, Shinozaki K. 1998. Two Transcription Factors, DREB1 and DREB2, with an EREBP/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought- and Low-Temperature-Responsive Gene Expression, respectively, in Arabidopsis. Plant Cell 10: 1391- 1406.
  6. Nelson JC. 1997. QGENE: software for marker-based genomic analysis and breeding, Molecular Breeding 3: 239-245. https://doi.org/10.1023/A:1009604312050
  7. McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. 2002. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Research 9: 199-207. https://doi.org/10.1093/dnares/9.6.199
  8. Panaud O, Chen X, McCouch SR. 1996. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Mol. Gen. Genet. 252: 597-607.
  9. Price AH, Townend J, Jones MP, Audebert A, Courtois B. 2002. Mapping QTLs associated with drought avoidance in uplandrice grown in the Philippines and West Africa. Plant Mol. Biol. 48: 683-695. https://doi.org/10.1023/A:1014805625790
  10. Tanksley SD. 1993. Mapping polygemes. Ann. Rec. Genet. 27: 205-233. https://doi.org/10.1146/annurev.ge.27.120193.001225
  11. Temnykh S, Cartinhour S, Park W, Ayres N, Hauck N, Lipovich L, Cho YG, McCouch SR. 2000. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor. Appl. Genet. 100: 697-712. https://doi.org/10.1007/s001220051342
  12. Temnykh S, De Clerck G, Lukashova A, Lipovitch L, Cartinhour S, McCouch SR. 2001. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency length variation, transposon associations, and genetic marker potential. Genome Res. 11: 1441-1452. https://doi.org/10.1101/gr.184001
  13. Thanh PT, Phan PDT, Mori N, Ishikawa R, Ishii T. 2011. Development of backcross recombinant inbred lines between Oryza sativa Nipponbare and O. rufipogon and QTL detection on drought tolerance. Breeding Sci. 61: 76-79. https://doi.org/10.1270/jsbbs.61.76
  14. Widawsky DA, O'Toole JC. 1996. Prioritizing the rice research agenda for Eastern India. In: R. Evenson, R. Herdt and M. Hossain (eds.) Rice Research in Asia: Progress and Priorities. CAB International, Wallingford, UK, pp. 109-129.
  15. Yano M, Sasaki T. 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Mol. Biol. 35: 145-153. https://doi.org/10.1023/A:1005764209331
  16. Yue B, Xiong L, Xue W, Xing Y, Luo L, Xu C. 2005. Genetic analysis for drought resistance of rice at reproductive stage in field with different types of soil. Theor. Appl. Genet. 111: 1127-1136. https://doi.org/10.1007/s00122-005-0040-1
  17. Zhang X, Zhou A, Fu Y, Su Z, Wang X, Sun C. 2006. Identification of a drought tolerant introgression line derived from Dongxiang common wild rice (O. rufipogon Griff.). Plant. Mol. Biol. 62: 247-259. https://doi.org/10.1007/s11103-006-9018-x