Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Diagnosis of Bovine Leukemia Virus (BLV) infection using PCR and ELISA techniques in Holstein dairy cattle

홀스타인종 젖소에 있어서 PCR과 ELISA기법을 이용한 BLV 감염진단

  • Jeong, Hang-Jin (Dept. of Animal Biosystem Science, Chungnam National University) ;
  • Yu, Seong-Lan (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Lee, Jun-Heon (Dept. of Animal Science and Biotechnology, Chungnam National University) ;
  • Do, Chang-Hee (Dept. of Animal Biosystem Science, Chungnam National University) ;
  • Shu, Guk-Hyun (College of Veterinary Medicine, Cheunnam National University) ;
  • Ryoo, Seung-Heui (Government of Changcheongnam-Do, Livestock Research Institute) ;
  • Sang, Byung-Chan (Dept. of Animal Biosystem Science, Chungnam National University)
  • 정행진 (충남대학교 동물바이오시스템과학과) ;
  • 유성란 (충남대학교 동물자원생명과학과) ;
  • 이준헌 (충남대학교 동물자원생명과학과) ;
  • 도창희 (충남대학교 동물바이오시스템과학과) ;
  • 서국현 (전남대학교 수의과대학) ;
  • 류승희 (충남 축산기술연구소) ;
  • 상병찬 (충남대학교 동물바이오시스템과학과)
  • Received : 2011.02.10
  • Accepted : 2011.03.09
  • Published : 2011.03.30
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Abstract

This study was conducted to investigate the farm situation about bovine leukemia virus(BLV) infection that greatly influence productivity in dairy cattle and compare the accuracy of diagnosis for BLV infection between PCR and ELISA techniques. Blood samples of 193 heads from 5 herds in Chungnam and Chungbuk area were used to analyze BLV gene and serum, and the results were obtained as follows. The amplified BLV gene in dairy cattle by PCR technique resulted in 226 bp, 596 bp and 434 bp, respectively, for gag, pol and env, which were well amplified. The infection rates of BLV virus diagnosed by PCR and ELISA techniques ranged from 80.55 to 100% and from 22.22 to 86.95%, respectively, and the infection rates among 5 herds were significantly different in both methods (P<0.05). Further, the average infection rates of 5 herds were 87.05 and 63.21%, respectively, for PCR and ELISA techniques. Kappa statistics for examining consistency of diagnosis by PCR and ELISA techniques showed 0.246, which represents low consistency. Consequently, PCR based BLV technique was considered as a corrective measure for diagnosis of BLV infection in Holstein dairy cattle.

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References

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