Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Waste Mushroom bed from Agaricus bisporus

양송이 수확 후 배지로부터 식물생장촉진세균의 분리 및 생육특성

  • Jung, Young-Pil (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Kyung, Ki-Cheon (Taean Lily Experiment Station, Chungcheongnam-do Agricultural Research and Extention Services) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 정영필 (충남대학교 생물환경화학과) ;
  • 경기천 (충남농업기술원 태안백합시험장) ;
  • 장갑열 (국립원예특작과학원 버섯과) ;
  • 윤민호 (충남대학교 생물환경화학과)
  • Received : 2011.09.23
  • Accepted : 2011.10.06
  • Published : 2011.10.31
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Abstract

An auxin-producing bacteria (3YN11-02) was isolated from waste mushroom bed from Agaricus bisporus of Chungnam Buyeo-Gun area. The strain 3YN11-02 was identified as a novel species belongs to Rahnella aquatica by a chemotaxanomic and phylogenetic nalysis. The isolate was confirmed to produce indole-3-acetic acid (IAA) which is one of auxin hormone by TLC and HPLC analysis. When the concentration of IAA was assessed by performing HPLC quantity analysis, the maximal $290mg\;L^{-1}$ of IAA detected in ether fraction extracted from the culture filtrate which was cultured in R2A broth containing 0.1% tryptophan for 24 h at $35^{\circ}C$. The molecular weight of the main peak obtained by LC-mass analysis was correspondent well to 175, that of IAA. To investigate the growth promoting effect of crop, when the culture broth of R. aquatica 3YN11-02 was infected onto water culture and seed pot of mung bean, the adventitious root induction and root growth of mung bean were 2.0 times higher than control.

Keywords

References

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