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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Purification of Peat Moss Extract Using a Supercritical CO 2 and Verification of Its Biological Activities

초임계 이산화탄소를 이용한 피트모스 추출물의 정제 및 생리활성 검증

  • Received : 2011.09.26
  • Accepted : 2011.10.24
  • Published : 2011.10.31
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Abstract

In this study, the canadian peat moss extract was purified by a supercritical 2 using three different conditions and assessed its biological activities. Peat moss was extracted by acid-alkaline extraction method (sample 1) and purified by a supercritical $CO_2$ at $40^{\circ}C$ under pressure of 100 bar (sample 2), 120 bar (sample 3) or 150 bar (sample 4). We evaluated the antioxidant activities of the samples by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, $Fe^{2+}$/ascorbate (FTC) and 2-thiobarbituric acid (TBA) methods. The antioxidant activities were examined by comparing the results with that of ascorbic acid as a positive control. Sample 3 showed relatively higher DPPH radical-scavenging activities than other samples. The antioxidant activity by FIC method exhibited similar results as the DPPH radical-scavenging activities. On the other hand, sample 2 showed higher antioxidant activity measured by TBA method of all. The whitening effects of the samples were examined using mushroom tyrosinase and B16F10 melanoma cells. Sample 3 exhibited overall significant whitening effects, however, other samples showed relatively lower effects. These results suggest that the peat moss extract purified by a supercritical 2 could be used as a cosmetic ingredient for the anti-aging and whitening effects.

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References

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