Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Genotype of Group A Rotavirus Isolated in Acute Gastroenteritis Patients and Groundwater in Seoul, Korea

서울지역 급성위장관염 환자 및 지하수에서 분리한 A형 로타바이러스의 유전자형

  • Kim, Eun-Jeung (Virus Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Kim, Moo-Sang (Virus Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Chae, Young-Zoo (Virus Team, Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Cheon, Doo-Sung (Center for Infectious Diseases, Korea National Institute of Health, Division of Enteric and Hepatitis Viruses)
  • 김은정 (서울시보건환경연구원) ;
  • 김무상 (서울시보건환경연구원) ;
  • 채영주 (서울시보건환경연구원) ;
  • 천두성 (질병관리본부 간염 폴리오팀)
  • Received : 2011.10.14
  • Accepted : 2011.11.18
  • Published : 2011.12.31
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Abstract

Fecal specimens from acute gastroenteritis in Seoul from 2009 to 2010 were collected and then tested for the presence of Group A Rotavirus by ELISA. Among a total of 1,916 samples investigated, 354 samples (18.4%) were positive. The predominant genotypes of positive samples were confirmed as P6G[4] (35%), P8G[3] (28%), P8G[1] (24%), P4G[2] (10%), P8G[9] (3%), respectively. Among a total of 70 ground water samples investigated, 2 samples (2.8%) were positive. The genotypes of positive samples were confirmed as P8G[3] (100%). By this molecular investigation, genotypic distribution associated with rotavirus will be used for control and prevention of rotavirus related diseases.

2009년부터 2010년까지 서울지역에서 산발적으로 발생한 급성위장관염 환자에 대하여 효소면역측정법(ELISA)을 이용하여 로타바이러스를 검출하였다. 총 1,916건 중 로타바이러스는 354건(18.4%)이 검출되었다. RT-PCR을 이용하여 유전자형 분석을 실시한 결과 P6G[4] (35%), P8G[3] (28%), P8G[1] (24%), P4G[2] (10%), P8G[9] (3%)로 나타났다. 서울지역 지하수 70지점을 대상으로 효소면역측정법을 이용하여 로타바이러스를 조사한 결과 2 지점에서 양성으로 검출되었으며, RT-PCR을 이용하여 유전자형 분석을 실시한 결과 P8G[3]로 나타났다. 이러한 연구결과는 서울지역 로타바이러스 질환의 예방을 위한 기초자료로 활용될 것으로 기대된다.

Keywords

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