Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Characterization and Antimicrobial Activity of Lactic Acid Bacteria Isolated from Vaginas of Women of Childbearing Age

가임기 여성의 질에서 분리한 젖산 세균인 Lactobacillus plantarum UK-3의 특성 및 항균활성

  • Ahn, Hye-Ran (Department of Biotechnology, Soonchunhyang University) ;
  • So, Jae-Seong (Department of Biological Engineering, Inha University) ;
  • Oh, Kye-Heon (Department of Biotechnology, Soonchunhyang University)
  • 안혜란 (순천향대학교 생명공학과) ;
  • 소재성 (인하대학교 생물공학과) ;
  • 오계헌 (순천향대학교 생명공학과)
  • Received : 2011.11.10
  • Accepted : 2011.12.14
  • Published : 2011.12.31
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Abstract

The purpose of this work was to examine the antimicrobial activity derived from the lactic acid bacterium, UK-3 isolated from the vaginas of women of childbearing age. Various physiological and biochemical properties of this strain were characterized. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Lactobacillus plantarum UK-3, and registered in GenBank as [JK266589]. Growth rate, production of organic acids (e.g., lactic acid and acetic acid), and pH during growth were monitored. The maximum concentrations of lactic acid and acetic acid were approximately 684.11 mM and 174.26 mM, respectively, and pH changed from 7.0 to 3.7 after 72 h of incubation. High performance liquid chromatography was used to confirm lactic acid and acetic acid production. Significant antimicrobial activity of the concentrated supernatant was demonstrated against various Gram-positive (e.g., Staphylococcus aureus, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, Neisseria species., Listeria monocytogenes), Gram-negative bacteria (e.g., Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis), and yeast (e.g., Candida albicans) by the plate diffusion method. As a result, the concentrated L. plantarum UK-3 cultures had lower acidity and inhibited the growth of all microorganisms tested, whereas the growth of L. acidophilus was not affected.

본 연구는 가임기 여성의 질로부터 젖산균인 Lactobacillus plantarum UK-3를 분리하여 다양한 생리화학적 특성조사와 여러 가지 병원균들에 대한 항균활성을 확인하고자 실시하였다. 균주 UK-3는 MRS 배지에서 배양되었으며, 형태학적 관찰 및 생화학적 특성을 조사하였으며, 16S rRNA 염기서열 분석을 통해 균주를 동정하여, Lactobacillus plantarum UK-3로 명명하였으며, GenBank에 [JK266589]로 등록하였다. 배양기간 동안에 L. plantarum UK-3의 생장과 유기산의 생성, pH 변화를 조사하였으며, HPLC를 사용하여 대사산물로서 생성된 젖산(lactic acid)과 아세트산(acetic acid)을 정량 및 정성분석 하였다. 이들 유기산의 생성은 L. plantarum UK-3의 생장과 비례하였고, 배양 48시간 경과 후에 젖산과 아세트산의 농도는 각각 약 684.11 mM과 174.26 mM이었으며, 초기 pH 7.0은 배양기간 동안 3.7로 감소하였다. 여성의 질에서 감염의 가능성이 있는 10가지의 그람양성 세균, 그람음성 세균, 그리고 효모에 대하여 25배로 농축된 배양상등액을 처리하여 plate diffusion assay 방법으로 항균활성을 측정하였다. 그 결과 본 연구에 사용된 10가지 미생물에 대해서 광범위하게 항균효과가 있는 것이 관찰되었으며, 정상균총으로서 다른 젖산세균인 Lactobacillus acidophilus에 대해서는 항균활성을 나타내지 않았다.

Keywords

References

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