Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimization of Culturing Conditions for Improved Production of Bioactive Metabolites by Pseudonocardia sp. VUK-10

  • Received : 2011.08.02
  • Accepted : 2011.08.23
  • Published : 2011.09.30
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Abstract

The purpose of the present study was to investigate the influence of cultural and environmental parameters affecting the growth and bioactive metabolite production of the rare strain VUK-10 of actinomycete Pseudonocardia, which exhibits a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was high the in modified yeast extract-malt extract-dextrose (ISP-2) broth, as compared to other tested media. Glucose (1%) and tryptone (0.25%) were found to be the most suitable carbon and nitrogen sources, respectively, for optimum production of growth and bioactive metabolites. Maximum production of bioactive metabolites was found in the culture medium with initial pH 7 incubated with the strain for four days at $30^{\circ}C$, under shaking conditions. This is the first report on the optimization of bioactive metabolites by Pseudonocardia sp. VUK-10.

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References

  1. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70:461-77.
  2. Baltz RH. Antibiotic discovery from actinomycetes: will a renaissance follow the decline and fall? SIM News 2005;55: 186-96.
  3. Nolan R, Cross T. 1988. Isolation and screening of actinomycetes. In: Goodfellow M, Williams ST, Mordarski M, editors. Actinomycetes in biotechnology. London: Academic Press; 1988. p. 1-32.
  4. Ratna Kala R, Chandrika V. Effect of different media for isolation, growth and maintenance of actinomycetes from mangrove sediments. Indian J Mar Sci 1993;2:297-9.
  5. Wang BS, Liang SC, Zhang WY, Zan QJ. Mangrove flora of the world. Acta Bot Sin 2003;45:644-53.
  6. Hong K, Yan B. Uncultured microorganisms in Hainan mangrove soil: diversity and functional genes. In: Liu SJ, Drake HL, editors. "Microbes and the environment: perspectives and challenges. Beijing: Science Press; 2008. p. 52-8.
  7. Han L, Huang XS, Sattler I, Fu HZ, Grabley S, Lin WH. Two new constituents from mangrove Bruguiera gymnorrhiza. J Asian Nat Prod Res 2007;9:327-31.
  8. Ara I, Kudo T, Matsumoto A, Takahashi Y, Omura S. Nonomuraea maheshkhaliensis sp. nov., a novel actinomycete isolated from mangrove rhizosphere mud. J Gen Appl Microbiol 2007;53:159-66.
  9. Thumar JT, Dhulia K, Singh, SP. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis strain Kut-8. World J Microbiol Biotechnol 2010;26:2081-7.
  10. Ismet A, Vikineswari S, Paramaswari S, Wong WH, Ward A, Seki T, Fiedler HP, Goodfellow M. Production and chemical characterization of antifungal metabolites from Micromonospora sp. M39 isolated from mangrove rhizosphere soil. World J Microbiol Biotechnol 2004;20:523-8.
  11. Maskey RP, Kock I, Helmke E, Laatsch H. Isolation and structure determination of phenazostatin D, a new phenazine derivative from a marine actinomycete isolate Pseudonocardia sp. B6273. Z Naturforsch B J Chem Sci 2003;58:692-4.
  12. Omura S, Tanaka H, Tanaka Y, Spiri-nakagawa P, Oiwa R., Takahashi Y, Matsuyama K, Iwai Y. Studies on bacterial cell wall inhibitors. VII. Azureomycins A and B, New antibiotics produced by Pseudonocardia azurea nov sp. Taxonomy of the producing organism, Isolation, Characterization and biological properties. J Antibiot 1997;32:985-94.
  13. Dekker KA, Inagaki T, Gootz TD, Huang LH, Kozima Y, Kohlbrenner WE, Matsunaga Y, McGuirk PR, Nomura E, Sakakibara T, et al. New quinolone compounds from Pseudonocardia sp. with selective and potent anti-Helicobacter pylori activity: taxonomy of producing strain, fermentation, isolation, structural elucidation and biological activities. J Antibiot (Tokyo) 1998;51:145-52.
  14. Lin J, Bai L, Deng Z, Zhong JJ. Effect of ammonium in medium on ansamitocin P-3 production by Actinosynnema pretiosum. Biotechnol Bioprocess Eng 2010;15:119-25.
  15. Waksman SA. Classification, identification and descriptions of genera and species. Vol. 2. Baltimore: Williams and Wilkins Co.; 1961.
  16. Westley JW, Evans RH, Sello LH, Proupe N, Liu LM, Blound JF. Isolation and characterization of antibiotic X- 14547A, a novel monocarboxylic acid ionophore produced by Streptomyces antibiotics NRRC 8167. J Antibiotics 1997; 32:100-07.
  17. Thakur D, Bora TC, Bordoloi GN, Maiumdar S. Influence of nutrition and culturing conditions for optimum growth and antimicrobial metabolite production by Streptomyces sp. 201. J Mycol Med 2009;19:161-7.
  18. Majumdar MK, Majumdar SK. Effect of minerals on neomycin production by Streptomyces fradiae. Appl Environ Microbiol 1967;13:190-3.
  19. Kathiresan K, Balagurunathan R, Selvam MM. Fungicidal activity of marine actinomycetes against phytopathogenic fungi. Indian J Biotechnol 2005;4:271-6.
  20. Narayana KJ, Vijayalakshmi M. Optimization of antimicrobial metabolites production by Streptomyces albidoflavus. Res J Pharmacol 2008;2:4-7.
  21. Oskay M. Effects of some environmental conditions on biomass and antimicrobial metabolite production by Streptomyces sp., KGG32. Int J Agric Biol 2011;13:317-24.
  22. Ripa FA, Nikkon F, Zaman S, Khondkar P. Optimal conditions for antimicrobial metabolites production from a new Streptomyces sp. RUPA-08PR isolated from Bangladeshi soil. Mycobiology 2009;37:211-4.
  23. Sujatha P, Bapi Raju KV, Ramana T. Studies on a new marine Streptomycete BT-408 producing polyketide antibiotic SBR-22 effective against methicillin resistant Staphylococcus aureus. Microbiol Res 2005;160:119-26.
  24. Kavitha A, Vijayalakshmi M. Cultural parameters affecting the production of bioactive metabolites by Nocardia levis MK-VL-113. J Appl Sci Res 2009;5:2138-47.
  25. Vasavada SH, Thumar JT, Singh SP. Secretion of a potent antibiotic by salt-tolerant and alkaliphilic actinomycete Streptomyces sannanensis strain RJT-1. Curr Sci 2006;91: 1393-7.
  26. Cruz R, Arias ME, Soliveri J. Nutritional requirements for the production of pyra zoloisoquinolinone antibiotics by Streptomyces griseocarneus NCIMB 40447. Appl Microbiol Biotechnol 1999;53:115-9.
  27. Wu JY, Huang JW, Shin HD, Lin WC, Liu YC. Optimization of cultivation conditions for fungichromin production from Streptomyces padanus PMS-702. J Chin Inst Chem Engin 2008;39:67-73.
  28. Singh LS, Mazumder S, Bora TC. Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D. J Mycol Med 2009;19:225-33.
  29. Atta HM, Bahobail AS, El-Sehrawi MH. Studies on isolation, classification and phylogenetic characterization of atifungal sbatanes produced by Streptomyces albidoflavus-143. New York Sci J 2011;40-53.
  30. Majumdar MK, Majumdar SK. Effect of minerals on neomycin production by Streptomyces fradiae. Appl Environ Microbiol 1965;13:190-3.