Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Distribution of Antibiotic Resistant Microbes in Aquaculture Effluent and Disinfection by Electron Beam Irradiation

양식장 배출수중의 항생제 내성균 분포 및 전자빔 살균처리

  • Jang, Eun-Hee (Radiation Research Division for Industry & Environment, Korea Atomic Energy Research Institute) ;
  • Lim, Seung-Joo (Radiation Research Division for Industry & Environment, Korea Atomic Energy Research Institute) ;
  • Kim, Tak-Hyun (Radiation Research Division for Industry & Environment, Korea Atomic Energy Research Institute)
  • 장은희 (한국원자력연구원 방사선공업환경연구부) ;
  • 임승주 (한국원자력연구원 방사선공업환경연구부) ;
  • 김탁현 (한국원자력연구원 방사선공업환경연구부)
  • Received : 2011.07.12
  • Accepted : 2011.07.26
  • Published : 2011.07.29
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Abstract

Antibiotic resistant microbes were isolated in catfish, trout, eel and loach aquaculture effluent. The distribution of antibiotic resistant microbes in aquaculture effluent and the disinfection efficiency of antibiotic resistant microbes by electron beam irradiation were investigated. It was shown that the multi-drug resistant bacteria were Aeromonas sp., Citrobacter sp., Bacillus sp., Marinobacter sp., Pantoea sp., Pseudomonas sp. and Enterobacter sp. in aquaculture effluent. 41.7% of total strains showed the resistance against one antibiotic agent, and 58.3% of total strains showed the resistance against more than two antibiotics. It was evidently shown that the toxicity and physicochemical properties of antibiotics can be estimated using Quantitative Structure Analysis Relationship (QSAR). Electron beam irradiation was very effective for the disinfection of antibiotic resistant bacteria from aquaculture effluent, in which the disinfection efficiency was approximately 99.9% with electron beam of 1 kGy.

육상양식장에서 항생제에 대한 내성을 가진 균을 분리하여 다양한 항생제에 대한 내성특성을 확인하고, 전자빔의 조사에 따른 내성균의 항생제 내성 특성변화와 살균효능에 관한 연구를 수행하였다. 메기, 송어, 뱀장어, 미꾸라지양식장의 배출수에서 분리된 균주 중 Aeromonas sp., Citrobacter sp., Bacillus sp., Marinobacter sp., Pantoea sp., Pseudomonas sp., Enterobacter sp.가 다제내성균으로 확인되었다. 양식장 배출수에서 분리한 균주 중 한가지의 항생제 내성을 가지고 있는 균은 41.7%이며, 2가지 이상의 항생제 내성을 가지고 있는 균은 58.3%로 나타났다. Quantitative Structure Analysis Relationship (QSAR) model에 의한 평가는 실험값과 매우 유사하여 독성 평가의 간접적인 지표로 이용될 수 있음을 알 수 있었다. 항생제 내성균에 대해 전자빔을 조사한 결과 1 kGy 이내의 선량에서 살균효율이 99.9%로 강한 살균력을 확인할 수 있었다.

Keywords

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