The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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The effects of Hyunggaeyungyo-tang of suppression of iNOS production on RAW 264.7cell

Mouse cell에서 형개연교탕(荊芥連翹湯)의 iNOS 생성 억제 효과

  • 박정훈 (동국대학교 대학원 한의학과 안이비인후피부과학교실) ;
  • 김종채 (동국대학교 대학원 한의학과 안이비인후피부과학교실) ;
  • 홍승욱 (동국대학교 대학원 한의학과 안이비인후피부과학교실)
  • Received : 2011.03.12
  • Accepted : 2011.04.01
  • Published : 2011.04.25
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Abstract

Background and Objectives : The aim of this study was to investigate anti-inflammatory and anti-oxidant effects of Hyunggaeyungyo-tang(HYT) on RAW 264.7 cells. Material and Methods : Two types of experiments were implemented for this study: first, the experiment to study the anti-oxidant effect of HYT using riboflavin; second, in vitro experiment to investigate the suppression of NF-${\kappa}$B activation using RAW 264.7 cells (I${\kappa}$B kinase and induce nitric oxide synthase mRNA expression) Results : 1. The anti-oxidant effects of HYT was dose-dependantly increased. 2. The RAW 264.7cells were treated with LPS for 1 hours prior to the addition of indicated concentrations(0.4,-1.0mg/$m\ell$) of HYT, and the cells were further incubated for 24 hours. The LPS-induced IKK & iNOS mRNA expression were dose-dependantly decreased in HYT treated RAW 264.7cells. Conclusion : The results suggest that HYT is significantly effective in the treatment of inflammation through the suppression of NF-${\kappa}$B activation and iNOS production.

Keywords

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