BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Human HS1BP3 induces cell apoptosis and activates AP-1

  • Shi, Taiping (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center) ;
  • Xie, Jieshi (Chinese National Human Genome Center) ;
  • Xiong, Ying (Chinese National Human Genome Center) ;
  • Deng, Weiwei (Chinese National Human Genome Center) ;
  • Guo, Jinhai (Chinese National Human Genome Center) ;
  • Wang, Feng (Chinese National Human Genome Center) ;
  • Ma, Dalong (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center)
  • Received : 2011.01.24
  • Accepted : 2011.04.03
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription.

Keywords

References

  1. Fischer, U., Michel, A. and Meese, E. U. (2005) Expression of the gene for hematopoietic cell specific protein is not restricted to cells of hematopoietic origin. Int. J. Mol. Med. 15, 611-615.
  2. Muzio, M., Scielzo, C., Frenquelli, M., Bachi, A., De Palma, M., Alessio, M., Ghia, P. and Caligaris-Cappio, F. (2007) HS1 complexes with cytoskeleton adapters in normal and malignant chronic lymphocytic leukemia B cells. Leukemia 21, 2067-2070. https://doi.org/10.1038/sj.leu.2404744
  3. Takemoto, Y., Furuta, M., Sato, M., Kubo, M. and Hashimoto, Y. (1999) Isolation and characterization of a novel HS1 SH3 domain binding protein, HS1BP3. Int. Immunol. 11, 1957-1964. https://doi.org/10.1093/intimm/11.12.1957
  4. Keeling, B. H., Vilariño-Güell, C., Ross, O. A., Wszolek, Z. K., Uitti, R. J. and Farrer, M. J. (2009) DRD3 Ser9Gly and HS1BP3 Ala265Gly are not associated with Parkinson disease. Neurosci. Lett. 461, 74-75. https://doi.org/10.1016/j.neulet.2009.05.084
  5. Deng, H., Le, W. D., Guo, Y., Huang, M. S., Xie, W. J. and Jankovic, J. (2005) Extended study of A265G variant of HS1BP3 in essential tremor and Parkinson disease. Neurology 65, 651-652. https://doi.org/10.1212/01.wnl.0000173033.32535.23
  6. Watabe, M., Ito, K., Masuda, Y., Nakajo, S. and Nakaya, K. (1998) Activation of AP-1 is required for bufalin-induced apoptosis in human leukemia U937 cells. Oncogene 16, 779-787. https://doi.org/10.1038/sj.onc.1201592
  7. Herdegen, T. and Waetzig, V. (2001) AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration. Oncogene 20, 2424-2437. https://doi.org/10.1038/sj.onc.1204387
  8. Angel, P., Szabowski, A. and Schorpp-Kistner, M. (2001) Function and regulation of AP-1 subunits in skin physiology and pathology. Oncogene 20, 2413-2423. https://doi.org/10.1038/sj.onc.1204380
  9. Ashida, R., Tominaga, K., Sasaki, E., Watanabe, T., Fujiwara, Y., Oshitani, N., Higuchi, K., Mitsuyama, S., Iwao, H. and Arakawa, T. (2005) AP-1 and colorectal cancer. Inflammopharmacology 13, 113-125. https://doi.org/10.1163/156856005774423935
  10. Matthews, C. P., Colburn, N. H. and Young, M. R. (2007) AP-1 a target for cancer prevention. Curr. Cancer Drug Targets 7, 317-324. https://doi.org/10.2174/156800907780809723
  11. Ham, J., Babij, C., Whitfield, J., Pfarr, C. M., Lallemand, D., Yaniv, M. and Rubin, L. L. (1995) A c-Jun dominant negative mutant protects sympathetic neurons against programmed cell death. Neuron 14, 927-939. https://doi.org/10.1016/0896-6273(95)90331-3
  12. Estus, S., Zaks, W. J., Freeman, R. S., Gruda, M., Bravo, R. and Johnson, E. M. Jr. (1994) Altered gene expression in neurons during programmed cell death: identification of c-jun as necessary for neuronal apoptosis. J. Cell Biol. 127, 1717-1727. https://doi.org/10.1083/jcb.127.6.1717
  13. Bossy-Wetzel, E., Bakiri, L. and Yaniv, M. (1997) Induction of apoptosis by the transcription factor c-Jun. EMBO J. 16, 1695-1709. https://doi.org/10.1093/emboj/16.7.1695
  14. Sawai, H., Okazaki, T., Yamamoto, H., Okano, H., Takeda, Y., Tashima, M., Sawada, H., Okuma, M., Ishikura, H., Umehara, H. and Domae, N. (1995) Requirement of AP-1 for ceramide-induced apoptosis in human leukemia HL-60 cells. J. Biol. Chem. 270, 27326-27331. https://doi.org/10.1074/jbc.270.45.27326
  15. Higgins, J. J., Lombardi, R. Q., Pucilowska, J., Jankovic, J., Tan, E. K. and Rooney, J. P. (2005) A variant in the HS1-BP3 gene is associated with familial essential tremor. Neurology 64, 417-421. https://doi.org/10.1212/01.WNL.0000153481.30222.38
  16. Fukuda, T., Kitamura, D., Taniuchi, I., Maekawa, Y., Benhamou, L. E., Sarthou, P. and Watanabe, T. (1995) Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate. Proc. Natl. Acad. Sci. U.S.A. 92, 7302-7306. https://doi.org/10.1073/pnas.92.16.7302
  17. Yamanashi, Y., Fukuda, T., Nishizumi, H., Inazu, T., Higashi, K., Kitamura, D., Ishida, T., Yamamura, H., Watanabe, T. and Yamamoto, T. (1997) Role of tyrosine phosphorylation of HS1 in B cell antigen receptor-mediated apoptosis. J. Exp. Med. 185, 1387-1392. https://doi.org/10.1084/jem.185.7.1387
  18. Chen, Y. R., Kori, R., John, B. and Tan, T. H. (2001) Caspase-mediated cleavage of actin-binding and SH3-domaincontaining proteins cortactin, HS1, and HIP-55 during apoptosis. Biochem. Biophys. Res. Commun. 288, 981-989. https://doi.org/10.1006/bbrc.2001.5862
  19. Ensenat, D., Yao, Z., Wang, X. S., Kori, R., Zhou, G., Lee, S. C. and Tan, T. H. (1999) A novel Src homology 3 domain- containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1. J. Biol. Chem. 274, 33945-33950. https://doi.org/10.1074/jbc.274.48.33945
  20. Tournier, C., Hess, P., Yang, D. D., Xu, J., Turner, T. K., Nimnual, A., Bar-Sagi, D., Jones, S. N., Flavell, R. A. and Davis, R. J. (2000) Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science 288, 870-874. https://doi.org/10.1126/science.288.5467.870
  21. Lei, K., Nimnual, A., Zong, W. X., Kennedy, N. J., Flavell, R. A., Thompson, C. B., Bar-Sagi, D. and Davis, R. J. (2002) The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH(2)-terminal kinase. Mol. Cell. Biol. 22, 4929-4942. https://doi.org/10.1128/MCB.22.13.4929-4942.2002

Cited by

  1. Mitochondria-associated ER membranes in Alzheimer disease vol.55, 2013, https://doi.org/10.1016/j.mcn.2012.07.011
  2. Genetics of essential tremor: From phenotype to genes, insights from both human and mouse studies vol.119-120, 2014, https://doi.org/10.1016/j.pneurobio.2014.05.001