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The Change in Biological Activities of Brown Rice and Germinated Brown Rice

품종별 현미 발아 전후의 생리활성물질 변화

  • Kim, Dae-Jung (National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Sea-Kwan (National Institute of Crop Science, Rural Development Administration) ;
  • Yoon, Mi-Ra (National Institute of Crop Science, Rural Development Administration) ;
  • Chun, A-Reum (National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Im-Soo (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Dong-Hyun (Misillan Company) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Yu, Kwang-Won (Division of Food and Biotechnology, Chungju National University) ;
  • Kim, Yeon-Kyu (National Institute of Crop Science, Rural Development Administration)
  • 김대중 (농촌진흥청 국립식량과학원) ;
  • 오세관 (농촌진흥청 국립식량과학원) ;
  • 윤미라 (농촌진흥청 국립식량과학원) ;
  • 천아름 (농촌진흥청 국립식량과학원) ;
  • 최임수 (농촌진흥청 국립식량과학원) ;
  • 이동현 ((주)미실란) ;
  • 이준수 (충북대학교 식품공학과) ;
  • 유광원 (충주대학교 식품영양학과) ;
  • 김연규 (농촌진흥청 국립식량과학원)
  • Received : 2011.03.10
  • Accepted : 2011.05.13
  • Published : 2011.06.25

Abstract

We studied the biological activities, including antioxidant compounds, antioxidant activities, anti-proliferative activities, and immunological activities of brown rice and germinated brown rice. We examined the DPPH, ABTS radical scavenging activity, and reducing power of 70% ethanol extracts from some cultivars of brown rice and germinated brown rice. The total polyphenol, total flavonoid, and ${\gamma}$-oryzanol contents of the extracts were measured with spectrophotometric methods. The Hongjinjubeyo brown rice and germinated brown rice extracts showed markedly higher antioxidative activity than those of 70% ethanol extracts from other cultivars. The 70% ethanol extracts from brown rice and germinated brown rice had the most effective anti-proliferative activity (cytotoxicity) against breast cancer cells (MCF-7) compared to colorectal cancer cells (HCT-116). A $500\;{\mu}g$/mL concentration of 70% Hongjinjubyeo ethanol extract had higher macrophage and mitogenic activities of immunological activity than other cultivars.

벼 품종별 발아 전후의 70% 에탄올 추출물에 대한 항산화성분(폴리페놀, 플라보노이드), 항산화 활성(ABTS 라디칼 소거능, DPPH 라디칼 소거능, 환원력), 항암활성 및 면역활성을 비교 분석하여 기능적 가치를 평가함으로써 이용 가능성을 살펴보고자 하였다. 그 결과 총 폴리페놀 함량은 발아 전후 상관없이 홍진주벼에서 각각 $5,600.44\;{\mu}g$ GAE/g sample과 $4,599.52\;{\mu}g$ GAE/g sample로 가장 높게 측정되었으며 총 플라보노이드 함량은 유색미인 홍진주벼에서 $1,841.17\;{\mu}g$ GAE/g sample(현미), $1,296.77\;{\mu}g$ CA/g sample(발아현미)에서 높게 나타났다. ${\gamma}$-Oryzanol 함량은 홍진주벼에서 $643.14\;{\mu}g/g$ sample로서 가장 높게 측정되었다. 품종별 발아 전후의 70% 에탄올 추출물에 대한 ABTS 라디칼 소거능, DPPH 라디칼 소거능, 환원력은 1 mg/mL의 농도에서 측정한 결과 유색미인 홍진주벼와 흑광벼의 현미에서 높은 활성을 나타내었다. 70% 에탄올 추출물에 대한 in vitro 항암활성을 측정한 결과 대장암 세포보다는 유방암 세포에서 더 강한 암세포 억제능을 관찰할 수 있었으며 발아현미보다 일반현미에서 다소 나은 결과를 나타내었다. 또한 $500\;{\mu}g$/mL의 농도에서 면역활성을 측정한 결과 유색미인 홍진주벼에서 발아 전후 상관없이 조사되어진 다른 품종들보다 높은 활성을 보였다.

Keywords

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