YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-Inflammatory Effects of Rice Bran Ethanol Extract in Murine Macrophage RAW 264.7 Cells

미강에탄올추출물의 RAW264.7 세포에서 항염증효과

  • Received : 2011.09.26
  • Accepted : 2011.11.03
  • Published : 2011.12.31
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Abstract

The aim of the present study is to investigate the anti-inflammatory effect of a Rice Bran Ethanol Extract (RBE). Inflammation, such as a bacterial infection in vivo metabolites, such as external stimuli or internal stimuli to the defense mechanisms of the biological tissue a variety of intracellular regulatory factors deulin inflammatory TNF-${\alpha}$, IL-$1{\beta}$, IL-6, IL-8, such as proinflammatory cytokines, prostagrandin, lysosomal enzyme, free radicals are involved in a variety of mediators. The present study was designed to determine the effect of the RBE on pro-inflammatory factors such as NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 in lipopolysaccharide (LPS) - stimulated RAW264.7 macrophages cells. The cell toxicity was determined by MTS assay. To evaluate of anti-inflammatory effect of RBE, amount of NO was measured using the NO detection kit and the iNOS expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). And proinflammatory cytokines were measured by ELISA kit. As a result, the RBE reduced NO, iNOS expression and TNF-${\alpha}$, IL-$1{\beta}$, IL-6 production without cytotoxicity. Our results suggest that the RBE may have an anti-inflammatory property through suppressing inflammatory mediator productions and appears to be useful as an anti-inflammatory material.

Keywords

References

  1. Shin, H. G. : Development and Research Trends of Functional Food. Food Science and Industry, Korean Society of Food Science and Technology 30, 2 (1997).
  2. Bae, S. M., Kim, J. H., Cho, C. W., Jeong, T. J., Yook, H. S., Byun, M. W. and Lee, S. C. : Effect of irradiation on the antioxidant activity of rice hull, rice bran and barley bran. J. Korean Soc. Food Sci. Nutr. 31, 246 (2002). https://doi.org/10.3746/jkfn.2002.31.2.246
  3. Qureshi, A. A., Mo, H., Packer, L. and Peterson, D. M. : Isolation and identification of novel tocotrienols from rice bran with hypocholesterolemic, antioxidant and antitumor properties. J. Agric. Food Chem. 48, 3130 (2000). https://doi.org/10.1021/jf000099t
  4. Choi, H. I., Ye, E. J., Kim, S. J., Bae, M. J., Yee, S. T., Park, E. J. and Park, E. M. : Anticancer (in vitro) and antiallergy effects of rice bran extracts. J. Korean Soc. Food Sic. Nutr. 35, 1297 (2006). https://doi.org/10.3746/jkfn.2006.35.10.1297
  5. Osawa, T., Narashima, R., Kawakishi, S., Namaki, M. and Tashiro, T. : Antioxidative defense system in rice hull against damage caused by oxygen radicals. Agric. Biol. Chem. 49, 3085 (1985). https://doi.org/10.1271/bbb1961.49.3085
  6. Lai, P., Li, K. Y., Lu, S. and Chen, H. H. : Phytocoemicals and antioxidant properties of solvent extracts from Japonica rice dran. Food Chem. 117, 538 (2009). https://doi.org/10.1016/j.foodchem.2009.04.031
  7. Muramoto, G. and Kawamura, S. : Rice protein and antihypertensive peptide (angiotensin converting enzyme inhibitor) from rice. Nippon. Shokuhin. Kougyo. 34, 18 (1991).
  8. Lee, K. Y., Kim, J. H., Son, J. R. and Lee, J. S. : Detection and extraction condition of physiological functional compounds from bran of Heugjinju rice (Oryza sativa L). Korean J. Postharvest. Sci. Technol. 8, 296 (2001).
  9. Akihisa, T., Yasukawa, K., Yamaura, M., Ukiya, M., Kimura, Y., Shimizu, N. and Arai, K. : Triterpene alcohol and sterol ferulates from rice bran and their anti-inflammatory effects. J. Agric. Food Chem. 48, 2313 (2000). https://doi.org/10.1021/jf000135o
  10. Rocca, B. and FitzGerald, G. A. : Cyclooxygenases and prostaglandins shaping up the immune response. Int. Immunopharmacol. 2, 603 (2002). https://doi.org/10.1016/S1567-5769(01)00204-1
  11. Craing, C. : Introduction to CNS Pharmacology. In Craing C. Stitzel R(eds.), Modern Pharmacology, 4th ed. Boston: Little, Brown & Co. 329 (1994).
  12. MacSween, R. and Whaley, K. : Muir's Textbook of Pathology, 13th ed. London: Edward Arnold (1992).
  13. Kang-Rotondo, C. H., Major, S., Chiang, T. M., Myers, L. K. and Kang, E. S. : Upregulation of nitric oxide synthase in cultured human keratinocytes after ultraviolet B and bradykinin. Photodermatol. Photoimmunol. Photomed. 12, 57 (1996). https://doi.org/10.1111/j.1600-0781.1996.tb00176.x
  14. Seo, S. J., Choi, H. G., Chung, H. J. and Hong, C. K. : Time course of expression of mRNA of inducible nitric oxide synthase and generation of nitric oxide by ultraviolet B in keratinocyte cell lines. Br. J. Dermatol. 147, 655 (2002). https://doi.org/10.1046/j.1365-2133.2002.04849.x
  15. Shew, R. L., Papka, R. E., McNeill, D. L. and Yee, J. A. : NADPH-diaphorase- positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction. Peptides 14, 637 (1993). https://doi.org/10.1016/0196-9781(93)90157-C
  16. Kwqagkmata, H., Ochiai, H., Mantani, N. and Terasawa, K. : Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells, a murine macrophage cell line. Am. J. Chin. Med. 28, 217 (2000). https://doi.org/10.1142/S0192415X0000026X
  17. Weller, R. : Nitric oxide - a newly discovered chemical transmitter in human skin. Br. J. Dermatol. 137, 665 (1997). https://doi.org/10.1111/j.1365-2133.1997.tb01099.x
  18. Masaki, M., Matsushita, M. and Wakitani, K. : Inhibitory effect of JTE-522, a novel prostaglandin H synthase-2 inhibitor, on adjuvant-induced arthritis and bone changes in rats. Inflamm. Res. 47, 187 (1998). https://doi.org/10.1007/s000110050316
  19. Desai, A., Vyas, T. and Amiji, M. : Cyroroxicity and apoptosis enhancement in brain tumor cells upon coadministration of paclitaxel and ceramide in nanoemulsion formulations. J. Pharm. Sci. 97, 2745 (2008). https://doi.org/10.1002/jps.21182
  20. Wang, S., Chen, Y., He, D., He, L., Yang, Y., Chen, J. and Wang, X. : Inhibition of vascular smooth muscl cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J. Ethnopharmacol. 114, 458 (2007). https://doi.org/10.1016/j.jep.2007.08.039
  21. Iontcheva, I., Amar, S., Zawawi, K. H., Kantarci, A. and Van Dyke, T. E. : Role for moesin in lipopolysaccharide-stimulated signal transduction. Infect. Immun. 72, 2312 (2004). https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  22. Delanty, N. and Dichter, M. A. : Oxidative injury in the nervous system. Acta Neurol Scand. 98, 145 (1998). https://doi.org/10.1111/j.1600-0404.1998.tb07285.x
  23. Weisz, A., Ciatiello, L. and Esumi, H. : Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NGmonomethyl-L-arginine. Biochem. J. 316, 209 (1996). https://doi.org/10.1042/bj3160209
  24. Moncada, S., Palmer, R. M. and Higgs, E. A. : Nitric oxide: physiology. Pathophysiology, and pharmacology. Pharmacol. Rev. 43, 109 (1991).
  25. Choi, S. P., Kang, M. Y. and Nam, S. H. : Inhibitory activity of the extracts from the pigmented rice brans on inflammatory reactions. J. Korean Soc. Appl. Biol. Chem. 47, 222 (2004).
  26. Hseu, Y. C., Wu, J. Y., Chang, W. H. and Lu, F. J. : Antiinflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway. Int. Inmunnopharmacol. 5, 1914 (2005). https://doi.org/10.1016/j.intimp.2005.06.013
  27. Sarkar, D., Saha, P., Gamre, S., Bhattacharjee, S., Hariharan, C. and Ganguly, S. : Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-${\kappa}B$ pathway. Int. Immunopharmacol. 8, 1264 (2008). https://doi.org/10.1016/j.intimp.2008.05.003
  28. Chae, S. W., Kim, J. S., Kang, K. A., Bu, H. D., Lee, Y. and Hyun, J. W. : Antioxidant activity of Jionoside D from Clerodendendron trichotomum. Biol. Pharm. Bull. 27, 1504 (2004). https://doi.org/10.1248/bpb.27.1504
  29. Hu, C., Zawistowski, J., Ling, W. and Kitts, D. : Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J. Agric. Food Chem. 51, 5271 (2003). https://doi.org/10.1021/jf034466n
  30. Nagasakaa, R., Chotimarkorna, C., Shafiqulb, I., Horib, M., Ozakib, H. and Ushioa, H. : Anti-inflammatory effects of hydrocinnamic acid derivatives. Biochemical and Biophysical Research Communications 358, 615 (2007). https://doi.org/10.1016/j.bbrc.2007.04.178
  31. 대한병리학회 병리학(3판). 서울: 고문사 65, 81 (1997).
  32. Lee, A. K., Sung, S. H., Kim, Y. C. and Kim, S. G. : Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-$\alpha$ and COX-2 expresstion by sauchinone effects on I-Ba phosphorylation, C/EBP and AP-1 activation. British journal of Pharmacology 139, 11 (2003). https://doi.org/10.1038/sj.bjp.0705231
  33. Islam, M. S., Murata, T., Fujisawa, M., Nagasaka, R., Ushio, H., Bari, A. M., Hori, M. and Ozaki, H. : Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. British Journal of Pharmacology 154, 812 (2008).