Maxillofacial Plastic and Reconstructive Surgery

  • 제33권4호
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  • Pages.301-307
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  • 2011
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

Response of Odontoblast to the Bio-Calcium Phosphate Cement

  • Kim, Jin-Woo (Department of Dentistry, College of Medicine, Dong-A University) ;
  • Kim, Sung-Won (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Gyoo-Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Yong-Deok (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Cheol-Hun (Department of Dentistry, College of Medicine, Dong-A University) ;
  • Kim, Bok-Joo (Department of Dentistry, College of Medicine, Dong-A University) ;
  • Kim, Uk-Kyu (Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 투고 : 2011.03.29
  • 심사 : 2011.05.12
  • 발행 : 2011.07.31
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초록

Purpose: If the tooth structure is damaged, then it is impossible to regenerate the tooth. The materials used to restore the tooth structure are not related to the composition of the tooth. The materials used to restore the structure can't replace the natural tooth because they just fill the defective structure. Calcium phosphate cement remineralizes the dentin and almost replaces the natural tooth, but there are some disadvantages. We conducted basic tests with Biomimetic CPC (Bio-CPC) to make sure of the possibility of the biomaterial to remineralize the defective tooth structure. Methods: In this study, the bioactivity and biocompatibility of Bio-CPC were evaluated for its potential value as the bio-material for regeneration of damaged tooth structure by conducting a cell toxicity assay (WST-1 assay), a cytokinesis-block micronucleus assay, a chromosomal aberration test, total RNA extraction and RT-PCR on MDPC-23 mouse odontoblast-like cells. Results: The in vitro cytotoxicity test showed that the Bio-CPC was fairly cytocompatible for the MDPC-23 mouse odontoblast-like cells. Conclusion: Bio-CPC has a possibility to be a new biomaterial and further study of Bio-CPC is needed.

키워드

참고문헌

  1. Xu HH, Weir MD, Simon CG. Injectable and strong nanoapatite scaffolds for cell/growth factor delivery and bone regeneration. Dent Mater 2008;24:1212-22. https://doi.org/10.1016/j.dental.2008.02.001
  2. Ryu KS, Shim JH, Heo HY, Park CK. Therapeutic efficacy of injectable calcium phosphate cement in osteoporotic vertebral compression fractures: prospective nonrandomized controlled study at 6-month follow-up. World Neurosurg 2010; 73:408-11. https://doi.org/10.1016/j.wneu.2010.01.006
  3. Chau JY, Hutter JW, Mork TO, Nicoll BK. An in vitro study of furcation perforation repair using calcium phosphate cement. J Endod 1997;23:588-92. https://doi.org/10.1016/S0099-2399(06)81129-5
  4. Brown WE, Chow LC. A new calcium phosphate water-setting cement. In: Brown PW, editor. Cements research progress 1986. Westerville: American Ceramic Society, OH; 1987. p.352-79.
  5. Xu HH, Carey LE, Simon CG Jr, Takagi S, Chow LC. Premixed calcium phosphate cements: synthesis, physical properties, and cell cytotoxicity. Dent Mater 2007;23:433-41. https://doi.org/10.1016/j.dental.2006.02.014
  6. Carey LE, Xu HH, Simon CG Jr, Takagi S, Chow LC. Premixed rapid-setting calcium phosphate composites for bone repair. Biomaterials 2005;26:5002-14. https://doi.org/10.1016/j.biomaterials.2005.01.015
  7. Chohayeb AA, Chow LC, Tsaknis PJ. Evaluation of calcium phosphate as a root canal sealer-filler material. J Endod 1987;13:384-7. https://doi.org/10.1016/S0099-2399(87)80198-X
  8. Hong YC, Wang JT, Hong CY, Brown WE, Chow LC. The periapical tissue reactions to a calcium phosphate cement in the teeth of monkeys. J Biomed Mater Res 1991;25:485-98. https://doi.org/10.1002/jbm.820250406
  9. Friedman CD, Costantino PD, Jones K, Chow LC, Pelzer HJ, Sisson GA Sr. Hydroxyapatite cement. II. Obliteration and reconstruction of the cat frontal sinus. Arch Otolaryngol Head Neck Surg 1991;117:385-9. https://doi.org/10.1001/archotol.1991.01870160039005
  10. Sugawara A, Nishiyama M, Kusama K, et al. Histopathological reactions of calcium phosphate cement. Dent Mater J 1992;11:11-6. https://doi.org/10.4012/dmj.11.11
  11. Costantino PD, Friedman CD, Jones K, Chow LC, Sisson GA. Experimental hydroxyapatite cement cranioplasty. Plast Reconstr Surg 1992;90:174-85; discussion 186-91. https://doi.org/10.1097/00006534-199290020-00003
  12. Sugawara A, Kusama K, Nishimura S, et al. Histolopathologycal reactions to calcium phosphate cement for bone filling. Dent Mater J 1993;12:691-8.
  13. Costantino PD, Friedman CD. Synthetic bone graft substitutes. Otolaryngol Clin North Am 1994;27:1037-74.
  14. Bohner M. Calcium orthophosphates in medicine: from ceramics to calcium phosphate cements. Injury 2000;31(Suppl 4):37-47.
  15. Friedman CD, Costantino PD, Takagi S, Chow LC. Bone- Source hydroxyapatite cement: a novel biomaterial for craniofacial skeletal tissue engineering and reconstruction. J Biomed Mater Res 1998;43:428-32. https://doi.org/10.1002/(SICI)1097-4636(199824)43:4<428::AID-JBM10>3.0.CO;2-0
  16. Schmitz JP, Hollinger JO, Milam SB. Reconstruction of bone using calcium phosphate bone cements: a critical review. J Oral Maxillofac Surg 1999;57:1122-6. https://doi.org/10.1016/S0278-2391(99)90338-5
  17. Chang MC, DeLong R. Calcium phosphate formation in gelatin matrix using free ion precursors of Ca2+ and phosphate ions. Dent Mater 2009;25:261-8. https://doi.org/10.1016/j.dental.2008.07.005
  18. Xu HH, Quinn JB, Takagi S, Chow LC. Processing and properties of strong and non-rigid calcium phosphate cement. J Dent Res 2002;81:219-24. https://doi.org/10.1177/154405910208100315
  19. Carter DR, Hayes WC. Bone compressive strength: the influence of density and strain rate. Science 1976;194:1174-6. https://doi.org/10.1126/science.996549
  20. Lee SK, Lee SK, Lee SI, et al. Effect of calcium phosphate cements on growth and odontoblastic differentiation in human dental pulp cells. J Endod 2010;36:1537-42. https://doi.org/10.1016/j.joen.2010.04.027
  21. Liu C, Wang W, Shen W, Chen T, Hu L, Chen Z. Evaluation of the biocompatibility of a nonceramic hydroxyapatite. J Endod 1997;23:490-3. https://doi.org/10.1016/S0099-2399(97)80307-X
  22. Yu Y, Wang J, Liu C, et al. Evaluation of inherent toxicology and biocompatibility of magnesium phosphate bone cement. Colloids Surf B Biointerfaces 2010;76:496-504. https://doi.org/10.1016/j.colsurfb.2009.12.010
  23. Zhang W, Walboomers XF, Jansen JA. The formation of tertiary dentin after pulp capping with a calcium phosphate cement, loaded with PLGA microparticles containing TGF-beta1. J Biomed Mater Res A 2008;85:439-44.
  24. Boland EJ, MacDougall M, Carnes DL, Dickens SH. In vitro cytotoxicity of a remineralizing resin-based calcium phosphate cement. Dent Mater 2006;22:338-45. https://doi.org/10.1016/j.dental.2005.05.004
  25. Tang H, Xu Z, Liu C, Qin X, Wu B, Wu L. Preparation of CPC for tissue engineering artificial rib and a study on proliferation and adhesion of BMSCs on CPC. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2008;22:1238-41.
  26. Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell 2002;110:673-87. https://doi.org/10.1016/S0092-8674(02)00971-6
  27. Harvey Lodish, editor. Molecular cell biology. 5th ed. New York: WH Freeman WH and Co.; 2003. p.973.