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Efficiency of laser-assisted intracytoplasmic sperm injection in a human assisted reproductive techniques program

  • Choi, Kyoung-Hee (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Jung-Hyun (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Yang, Yun-Hee (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Yoon, Tae-Ki (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Dong-Ryul (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Woo-Sik (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine)
  • 투고 : 2011.05.31
  • 심사 : 2011.08.16
  • 발행 : 2011.09.30

초록

Objective: Laser-assisted intracytoplasmic sperm injection (LA-ICSI), also known as micro-opening or thinning of the zona pellucida (ZP) prior to ICSI, may help to reduce mechanical damage to the oocyte during the procedure. The aim of the present study was to evaluate and analyze the efficacy of our institutional LA-ICSI program, which features laser-assisted ZP thinning prior to ICSI, in comparison with conventional ICSI (C-ICSI), performed on patients with different clinical characteristics. Methods: Patients undergoing a total of 212 ICSI cycles were randomly divided into an LA-ICSI group (106 cycles) and a conventional ICSI group (106 cycles). To reduce tissue damage, we thinned the ZP by approximately 70%, using a laser, before ICSI. Patients thus treated formed the LAICSI group. Comparisons included the morphological quality of transferred embryos, blastocyst development of the remaining embryos, and clinical pregnancy, in terms of ICSI method and patient characteristics. Results: Fertilization, development of remaining embryos, and pregnancy rate were significantly higher in the LA-ICSI group compared with the C-ICSI group. Fertilization, embryonic development, and the pregnancy rate were all improved in younger patients (<38 years of age) and in those who underwent a low number of IVF-ET attempts (<3 trials). In addition, the pregnancy rate was increased in older patients. Conclusion: LA-ICSI may be useful in improving the chance of pregnancy in all ICSI patients.

키워드

참고문헌

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피인용 문헌

  1. Strategies to improve fertilisation rates with assisted conception: a systematic review vol.21, pp.4, 2011, https://doi.org/10.1080/14647273.2017.1324182
  2. A randomized controlled trial of laser-assisted ICSI vol.35, pp.12, 2020, https://doi.org/10.1093/humrep/deaa160
  3. Thoughts on the popularity of ICSI vol.38, pp.1, 2011, https://doi.org/10.1007/s10815-020-01987-0
  4. Oocyte Degeneration After ICSI Is Not an Indicator of Live Birth in Young Women vol.12, pp.None, 2011, https://doi.org/10.3389/fendo.2021.705733
  5. Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage vol.38, pp.2, 2011, https://doi.org/10.1007/s10815-020-01998-x
  6. From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology vol.11, pp.10, 2011, https://doi.org/10.3390/diagnostics11101897