Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Efficiency of laser-assisted intracytoplasmic sperm injection in a human assisted reproductive techniques program

  • Choi, Kyoung-Hee (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Jung-Hyun (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Yang, Yun-Hee (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Yoon, Tae-Ki (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Dong-Ryul (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) ;
  • Lee, Woo-Sik (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine)
  • Received : 2011.05.31
  • Accepted : 2011.08.16
  • Published : 2011.09.30
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Abstract

Objective: Laser-assisted intracytoplasmic sperm injection (LA-ICSI), also known as micro-opening or thinning of the zona pellucida (ZP) prior to ICSI, may help to reduce mechanical damage to the oocyte during the procedure. The aim of the present study was to evaluate and analyze the efficacy of our institutional LA-ICSI program, which features laser-assisted ZP thinning prior to ICSI, in comparison with conventional ICSI (C-ICSI), performed on patients with different clinical characteristics. Methods: Patients undergoing a total of 212 ICSI cycles were randomly divided into an LA-ICSI group (106 cycles) and a conventional ICSI group (106 cycles). To reduce tissue damage, we thinned the ZP by approximately 70%, using a laser, before ICSI. Patients thus treated formed the LAICSI group. Comparisons included the morphological quality of transferred embryos, blastocyst development of the remaining embryos, and clinical pregnancy, in terms of ICSI method and patient characteristics. Results: Fertilization, development of remaining embryos, and pregnancy rate were significantly higher in the LA-ICSI group compared with the C-ICSI group. Fertilization, embryonic development, and the pregnancy rate were all improved in younger patients (<38 years of age) and in those who underwent a low number of IVF-ET attempts (<3 trials). In addition, the pregnancy rate was increased in older patients. Conclusion: LA-ICSI may be useful in improving the chance of pregnancy in all ICSI patients.

Keywords

References

  1. Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992;340:17-8. https://doi.org/10.1016/0140-6736(92)92425-F
  2. Ebner T, Moser M, Sommergruber M, Jesacher K, Tews G. Complete oocyte activation failure after ICSI can be overcome by a modified injection technique. Hum Reprod 2004;19:1837-41. https://doi.org/10.1093/humrep/deh325
  3. Ola B, Afnan M, Sharif K, Papaioannou S, Hammadieh N, Barratt CL. Should ICSI be the treatment of choice for all cases of in-vitro conception? Considerations of fertilization and embryo development, cost effectiveness and safety. Hum Reprod 2001;16:2485-90. https://doi.org/10.1093/humrep/16.12.2485
  4. Oehninger S, Gosden RG. Should ICSI be the treatment of choice for all cases of in-vitro conception? No, not in light of the scientific data. Hum Reprod 2002;17:2237-42. https://doi.org/10.1093/humrep/17.9.2237
  5. Bhattacharya S, Hamilton MP, Shaaban M, Khalaf Y, Seddler M, Ghobara T, et al. Conventional in-vitro fertilisation versus intracytoplasmic sperm injection for the treatment of non-male-factor infertility: a randomised controlled trial. Lancet 2001;357:2075-9. https://doi.org/10.1016/S0140-6736(00)05179-5
  6. Ebner T, Yaman C, Moser M, Sommergruber M, Jesacher K, Tews G. A prospective study on oocyte survival rate after ICSI: influence of injection technique and morphological features. J Assist Reprod Genet 2001;18:623-8. https://doi.org/10.1023/A:1013171505702
  7. Liu J, Nagy Z, Joris H, Tournaye H, Smitz J, Camus M, et al. Analysis of 76 total fertilization failure cycles out of 2732 intracytoplasmic sperm injection cycles. Hum Reprod 1995;10:2630-6. https://doi.org/10.1093/oxfordjournals.humrep.a135758
  8. Nagy ZP, Liu J, Joris H, Bocken G, Desmet B, Van Ranst H, et al. The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Hum Reprod 1995;10:3171-7. https://doi.org/10.1093/oxfordjournals.humrep.a135881
  9. Palermo GD, Alikani M, Bertoli M, Colombero LT, Moy F, Cohen J, et al. Oolemma characteristics in relation to survival and fertilization patterns of oocytes treated by intracytoplasmic sperm injection. Hum Reprod 1996;11:172-6. https://doi.org/10.1093/oxfordjournals.humrep.a019012
  10. Huang T, Kimura Y, Yanagimachi R. The use of piezo micromanipulation for intracytoplasmic sperm injection of human oocytes. J Assist Reprod Genet 1996;13:320-8. https://doi.org/10.1007/BF02070146
  11. Yanagida K, Katayose H, Yazawa H, Kimura Y, Konnai K, Sato A. The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum Reprod 1999;14:448-53. https://doi.org/10.1093/humrep/14.2.448
  12. Rienzi L, Greco E, Ubaldi F, Iacobelli M, Martinez F, Tesarik J. Laserassisted intracytoplasmic sperm injection. Fertil Steril 2001;76:1045-7. https://doi.org/10.1016/S0015-0282(01)02861-8
  13. Demirol A, Benkhalifa M, Sari T, Gurgan T. Use of laser-assisted intracytoplasmic sperm injection (ICSI) in patients with a history of poor ICSI outcome and limited metaphase II oocytes. Fertil Steril 2006;86:256-8. https://doi.org/10.1016/j.fertnstert.2005.11.078
  14. Abdelmassih S, Cardoso J, Abdelmassih V, Dias JA, Abdelmassih R, Nagy ZP. Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates. Hum Reprod 2002;17:2694-9. https://doi.org/10.1093/humrep/17.10.2694
  15. Moser M, Ebner T, Sommergruber M, Gaisswinkler U, Jesacher K, Puchner M, et al. Laser-assisted zona pellucida thinning prior to routine ICSI. Hum Reprod 2004;19:573-8. https://doi.org/10.1093/humrep/deh093
  16. Balakier H, Mandel R, Sojecki A, Motamedi G, Zaver S, Librach C. Laser zona thinning in women aged https://doi.org/10.1016/j.fertnstert.2008.07.1729
  17. Valojerdi MR, Eftekhari-Yazdi P, Karimian L, Ashtiani SK. Effect of laser zona pellucida opening on clinical outcome of assisted reproduction technology in patients with advanced female age, recurrent implantation failure, or frozen-thawed embryos. Fertil Steril 2008;90:84-91. https://doi.org/10.1016/j.fertnstert.2007.06.005
  18. Mantoudis E, Podsiadly BT, Gorgy A, Venkat G, Craft IL. A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum Reprod 2001;16:2182-6. https://doi.org/10.1093/humrep/16.10.2182
  19. Alikani M, Noyes N, Cohen J, Rosenwaks Z. Monozygotic twinning in the human is associated with the zona pellucida architecture. Hum Reprod 1994;9:1318-21. https://doi.org/10.1093/oxfordjournals.humrep.a138701
  20. da Costa AA, Abdelmassih S, de Oliveira FG, Abdelmassih V, Abdelmassih R, Nagy ZP, et al. Monozygotic twins and transfer at the blastocyst stage after ICSI. Hum Reprod 2001;16:333-6. https://doi.org/10.1093/humrep/16.2.333
  21. Schieve LA, Meikle SF, Peterson HB, Jeng G, Burnett NM, Wilcox LS. Does assisted hatching pose a risk for monozygotic twinning in pregnancies conceived through in vitro fertilization? Fertil Steril 2000;74:288-94. https://doi.org/10.1016/S0015-0282(00)00602-6

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