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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract

iNOS 발현 검출을 위한 in vitro 시스템의 확립 및 적송잎 추출물에 의한 저해효과 검증

  • Kim, Nam-Young (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Jang, Hye-Ji (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Jang, Min-Kyung (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Lee, Seung-Woo (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Jeon, Myong-Je (Department of Bioscience and Biotechnology, Graduate School, Silla University) ;
  • Kim, Mi-Hyang (Department of Food and Nutrition, Silla University) ;
  • Kim, Sung-Gu (Bioport Korea Co.) ;
  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, Graduate School, Silla University)
  • 김남영 (신라대학교 대학원 생명공학과) ;
  • 장혜지 (신라대학교 대학원 생명공학과) ;
  • 이동근 (신라대학교 대학원 생명공학과) ;
  • 장민경 (신라대학교 대학원 생명공학과) ;
  • 이승우 (신라대학교 대학원 생명공학과) ;
  • 전명제 (신라대학교 대학원 생명공학과) ;
  • 김미향 (신라대학교 식품영양학과) ;
  • 김성구 ((주)바이오포트 코리아) ;
  • 이상현 (신라대학교 대학원 생명공학과)
  • Received : 2011.03.10
  • Accepted : 2011.03.25
  • Published : 2011.04.30
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Abstract

This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 ${\mu}g/mL$ of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 ${\mu}g/mL$, respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.

Keywords

References

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