Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Antioxidative and Antimicrobial Activities of Castanopsis cuspidata var. sieboldii Extracts

구실잣밤나무 추출물의 항산화 및 항균 활성

  • 김지영 ((재)제주테크노파크 제주생물종다양성연구소) ;
  • 윤원종 ((재)제주테크노파크 제주생물종다양성연구소) ;
  • 임은영 ((재)제주테크노파크 제주생물종다양성연구소) ;
  • 박수영 ((재)제주테크노파크 제주생물종다양성연구소) ;
  • 김영주 ((재)제주테크노파크 제주생물종다양성연구소) ;
  • 송관필 ((재)제주테크노파크 제주생물종다양성연구소)
  • Received : 2010.11.08
  • Accepted : 2011.02.18
  • Published : 2011.04.30
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Abstract

This study was designed to investigate the possible utilization of Castanopsis cuspidata as a source of antiseptic agents. The leaves of C. cuspidata, extracted by 80% ethanol, were sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol. In order to effectively screen for a natural preservative agent, we first investigated the antioxidant activities such as DPPH radical scavenging capacity, superoxide radical scavenging capacity, and xanthine oxidase inhibitory activity of the C. cuspidata ethanol extracts and fraction. Using a screening system, we found that the ethylacetate fraction had the strongest antioxidant activity, which followed a dose-dependent manner. The antimicrobial activities were shown in the ethylacetate fraction of C. cuspidata. Among the five fractions, the ethylacetate fraction showed the highest antimicrobial activities against microorganisms tested, which were Bacillus sublitis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis and Salmonella typhimurium. In addition, leaf extracts of C. cuspidata could be suitable for the development of food preservatives.

본 연구는 구실잣밤나무를 식품 저장성이나 안전성을 향상하기 위한 식품 보존제로서의 개발 가능성을 알아보고자 하였다. 구실잣밤나무의 잎을 에탄올로 추출하고 헥산, 디클로로메탄, 에틸아세테이트, 부탄올로 순차적으로 용매분획하였다. 먼저, 항산화활성으로 DPPH 소거활성, superoxide radical 소거 활성 그리고 xanthine oxidase 억제 활성을 측정하였다. 그 결과, 에틸아세테이트 분획물에서 농도 의존적으로 가장 높은 항산화 활성을 나타내었다. 항균활성은 에탄올 추출물과 용매분획물을 농도별로 조사한 결과 다른 분획물에 비해 Bacillus sublitis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis 그리고 Salmonella typhimurium에서 에틸아세테이트 분획물에 높은 활성을 띠었다. 이상의 결과를 볼때, 구실잣밤나무 잎 추출물은 식품 보존제의 개발에 적합할 수 있다고 사료 된다.

Keywords

References

  1. Ak, T. and I. Gulcin. 2008. Antioxidant and radical scavenging properties of curcumin. Chem. Biol. Interact. 174:27-37. https://doi.org/10.1016/j.cbi.2008.05.003
  2. Al-Bayati, F.A. 2009. Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq. Ann. Clin. Microbiol. Antimicrob. 8:20. https://doi.org/10.1186/1476-0711-8-20
  3. Amster, D. 1996. Susceptibility testing of antimicrobial in liquid media, antibiotics in laboratory medicine. 4th ed. Williams and Wikins, MD, USA. pp. 52-111.
  4. Beuchat, L.R., and D.A. Golden. 1989. Antimicrobials occurring naturally in food. Food. Technol. 43:134-139.
  5. Cho, M.H., E.K. Bae, S.D. Ha and J.Y. Park. 2005. Application of natural antimicrobials to food industry. Food. Sci. Ins. 38:36-45 (in Korean).
  6. Choi, I., J.Y. Cho and S.C. Lim. 2006. Antimicrobial Activity of Medicinal Herbs against Staphylococcus aureus. Kor. J. Plant Res. 19:491-496 (in Korean).
  7. Choi, M.Y. and T.J. Rhim. 2008. Antimicrobial effect of Oregano (Origanum majorana L.) extract on food-bone pathogens. Kor. J. Plant Res. 21:352-356 (in Korean).
  8. Choi, Y, S.M. Lee, J. Chun, H.B. Lee and J. Lee. 2006. Influence of heat treatment on the antioxidant activities and polyphenolic compounds of Shiitake (Lentinus edodes) mushroom. Food. Chem. 99:381-387 (in Korean). https://doi.org/10.1016/j.foodchem.2005.08.004
  9. Greig, J.D. and A. Ravel. 2009. Analysis of foodborne outbreak data reported internationally for source attribution. Int. J. Food. Microbiol. 130:77-87. https://doi.org/10.1016/j.ijfoodmicro.2008.12.031
  10. Hayes, A.J. and B. Markovic. 2002. Toxicity of Australian essential oil Backhousia citriodora (Lemon myrtle). Part 11. Antimicrobial activity and in vitro cytotoxicity. Food. Chem. Toxicol. 40:535-543. https://doi.org/10.1016/S0278-6915(01)00103-X
  11. Kang, J.T., N.C. Park and Y.G. Chung. 2002. Effects of the soil properties on growth of Castanopsis cuspidata var. sieboldii and Dendropanax morbifera stands in warm temperate forest zone. J. Kor. For. Soc. 91:679-689 (in Korean).
  12. Kang, Y.J., G.P. Hong, H.J. Kwon, J.K. Hong, B.K. Park and D.H Oh. 2001. Antimicrobial activities of Quercus spp. leaf ethanol extracts against foodborne disease microorganisms. J. Kor. Soc. Food. Sci. Nutr. 30:415-420 (in Korean).
  13. Kim, H.Y., Y.J. Lee, S.H. Kim, K.H. Hong, Y.K. Kwon, J.Y. Lee, S.C. Ha, H.Y. Cho, I.S. Chang, C.W. Lee and K.S. Kim. 1999. Studies on the development of natural preservatives from natural products. Kor. J. Food. Sci. Technol. 31:1667-1678 (in Korean).
  14. Kim, J.Y., J.A. Lee, W.J. Yoon, D.J. Oh, Y.H. Jung, W.J. Lee and S.Y. Park. 2006. Antioxidative and antimicrobial activities of Euphorbia jolkini extracts. Kor. J. Food. Sci. Technol. 38:699-706 (in Korean).
  15. Kim, T.U. 1996. The forest of Korea. 1st ed. Kyohak Press Inc, Seoul, Korea. p. 20 (in Korean).
  16. Lee, B.W. and D.H. Shin. 1991. Antimicrobial effect of some plant extracts and their fractionates for food spoilage microorganisms. Kor. J. Food. Sci. Technol. 23:205-211 (in Korean).
  17. Lee, S.J., K.W. An, T.S. Choi, H.S. Jung, J.H. Moon and K.H. Park. 2010. Component analysis and antioxidative activity of Castanopsis cuspidata var. sieboldii nut. Kor. J. Food. Preserv. 17:139-144 (in Korean).
  18. Lee, T.B. 1979. Illustrated Flora of Korea. Hyangmoon Publishing Co., Seoul, Korea. p. 511 (in Korean).
  19. Lee, Y.N. 1998. Flora of Korea. 3th ed. Kyohak Publishing Co., Ltd., Seoul, Korea. p. 72 (in Korean).
  20. Liu, R.H. 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr. 134:3479-3485.
  21. Maregesi, S.M, L. Pieters, O.D. Ngassapa, S. Apers, R. Vingerhoets, P. Cos, D.A. Berghe and A.J. Vlietinck. 2008. Screening of some Tanzanian medicinal plants from Bunda district for antibacterial, antifungal and antiviral activities. J. Ethnopharmacol. 119:58-66. https://doi.org/10.1016/j.jep.2008.05.033
  22. Mothana, R.A. and U. Lindequist. 2005. Antimicrobial activity of some medicinal plants of the island Soqotra. J. Ethno-Pharmacol. 96:177-181. https://doi.org/10.1016/j.jep.2004.09.006
  23. Nara, K., T. Miyoshi, T. Honma and H. Koga. 2006. Antioxidative activity of bound-form phenolics in potato peel. Biosci. Biotechnol. Biochem. 70:1489-1491. https://doi.org/10.1271/bbb.50552
  24. Park, S.K. and J.C. Park. 1994. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Kor. J. Biotechnol. Bioeng. 9:506-511 (in Korean).
  25. Ryu, S.W., C.W. Jin, H.S. Lee, J.Y. Lee, K. Sapkota, B.G. Lee, C.Y. Yu, M.K. Lee, M.J. Kim and D.H. Cho. 2006. Changes in total polyphenol, total flavonoid contents and antioxidant activities of Hibiscus cannabinus L. Kor. J. Med. Crop. Sci. 14:307-310.
  26. Silva, E.G., G.A. Behr, A. Zanotto-Filho, R. Lorenzi, M.A. Pasquali, L.G. Ravazolo, C.L. Bordignon, F.A. Silva, A.L. Aboy, V.L. Bassani, A.T. Henriques, F.H. Reginatto, F. Dal-Pizzol and J.C. Moreira. 2007. Antioxidant activities and free radical scavenging potential of Bauhinia microstachya (RADDI) MACBR. (Caesalpinaceae) extracts linked to their polyphenol content. Biol. Pharm. Bull. 30:1488-1496. https://doi.org/10.1248/bpb.30.1488
  27. Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos. 1999. Analysis of total phenolic and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 299:152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
  28. Topisirovic, L., M. Kojic, D. Fira, N. Golic, I. Strahinic and J. Lozo. 2006. Potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. Int. J. Food. Microbiol. 112:230-235. https://doi.org/10.1016/j.ijfoodmicro.2006.04.009
  29. Valentova, K., F. Sersen and J. Ulrichova. 2005. Radical scavenging and anti-lipoperoxidative activities of Smallanthus sonchifolius leaf extracts. J. Agric. Food. Chem. 53:1577-1582.
  30. Yoon, J.H. 1994. Introduction of Forestry. 1st ed. Kangwon National University Academic Press Inc, Chunchon, Korea. p. 29 (in Korean).
  31. Yun, J.W., M.Y. Yoo, B.K. Park, M.K. Lee and D.H. Oh, 2004. Antimicrobial effect of ethanol extracts of Quercus spp. leaf against foodborne pathogens. J. Kor. Soc. Food. Sci. Nutr. 33:463-468 (in Korean). https://doi.org/10.3746/jkfn.2004.33.3.463

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