Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Plant Growth Regulators and Media on Regeneration of Sorghum bicolor (L.) Moench

바이오에너지용 수수 품종의 재분화율 증진을 위한 배지와 생장조절제 효과

  • Goh, Eun-Jeong (Bioherb Research Institute, Kangwon National University) ;
  • Seong, Eun-Soo (Bioherb Research Institute, Kangwon National University) ;
  • Yoo, Ji-Hye (Bioherb Research Institute, Kangwon National University) ;
  • Kil, Hyun-Young (Bioherb Research Institute, Kangwon National University) ;
  • Lee, Jae-Geun (Bioherb Research Institute, Kangwon National University) ;
  • Hwang, In-Seong (Bioherb Research Institute, Kangwon National University) ;
  • Kim, Nam-Jun (Bioherb Research Institute, Kangwon National University) ;
  • Ghimire, Bimal Kumar (Department of Applied Life Science, Konkuk University) ;
  • Kim, Myong-Jo (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lee, Ju-Kyung (Division of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Na-Young (Food Service Cuisine Songho College) ;
  • Yu, Chang-Yeon (Bioherb Research Institute, Kangwon National University)
  • 고은정 (강원대학교 한방바이오연구소) ;
  • 성은수 (강원대학교 한방바이오연구소) ;
  • 유지혜 (강원대학교 한방바이오연구소) ;
  • 길현영 (강원대학교 한방바이오연구소) ;
  • 이재근 (강원대학교 한방바이오연구소) ;
  • 황인성 (강원대학교 한방바이오연구소) ;
  • 김남준 (강원대학교 한방바이오연구소) ;
  • 비말 (건국대학교 응용생명과학과) ;
  • 김명조 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 이주경 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 임정대 (강원대학교 삼척캠퍼스 생약자원개발학과) ;
  • 김나영 (송호대학 외식조리과) ;
  • 유창연 (강원대학교 한방바이오연구소)
  • Received : 2010.08.09
  • Accepted : 2011.02.01
  • Published : 2011.04.30
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Abstract

This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 $mg\;l^{-1}$ 6-benzyl amino purine (BAP) and 0.25 $mg\;l^{-1}$ indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 $mg\;l^{-1}$) supplemented with IBA (0.25 $mg\;l^{-1}$) was effective combination for shoot multiplication. MS medium supplemented with 1 $mg\;l^{-1}$ IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor.

본 연구는 수수의 성숙 종자로부터 캘러스유기 및 재분화 효율 조사하기위하여 수행되었다. 배발생 캘러스 형성을 살펴보기 위해 생장조절제 효과를 조사하였다. 수수 성숙종자로부터 캘러스 유기에 적합한 생장조절제 종류와 농도는 2 mg/L 2,4-D인 것으로 나타났다. 배발생 캘러스의 고효율 빈도는 B5 배지에 2 mg/L 2,4-D을 첨가하였을 때 가장 양호한 것으로 나타났다. 배발생 캘러스로부터 식물체 재분화는 BAP와 IBA를 넣은 MS 배지가 가장 적절한 것으로 나타났다. BAP 농도가 높을수록 신초재분화가 활발하였다. 따라서, 본 연구 결과로부터 수수 기내배양시 신초와 발근 형성에 효과적인 생장조절제 조합은 0.5 $mg\;l^{-1}$ BAP와 0.25 $mg\;l^{-1}$ IBA인 것으로 나타났다.

Keywords

References

  1. Arlene, H., S. Shirley, D. Ismail, F. Mike and C. Tom. 2006. Rapid and reproducible Agrobacterium-mediated transformation of sorghum. Plant Cell Rep. 25:284-791.
  2. Bai, Z. L., L. Q. Wang, L. P. Zheng, A. J. Li and F. L. Wang. 1995. A study on the callus induction and plant regeneration of different sorghum explants. Acta Agriculturae Boreali Sinica 10:60-63.
  3. Baskaran, P. and N. Jayabalan. 2005. An efficient plant regeneration system for Sorghum bicolor a valuable major cereal crop. J. Plant Biotech. 7:247-257.
  4. Bhat, S. and M. S. Kuruvinashetti. 1995. Callus induction and plant regeneration from immature embryos of maintainer line (B) of kharif sorghum. J. Maharashtra Agricult. University 20:159.
  5. Cai, T. and L. Butler. 1990. Plant regeneration from embryogenic callus initiated from immature inflorescence of several high-tannin sorghums. Plant Cell Tiss. Org. Cult. 20:101-110. https://doi.org/10.1007/BF00114707
  6. Cho, N. K., Y. K. Kang, C. K. Song, Y. C. Jeun, J. S. Oh, Y. I. Cho and S. J. Park. 2004. Effects of planting density on growth, forage yield and chemical composition of Jeju native Sorghum (Sorghum bicolor L.). J. Kor. Grassl. Sci. 24:225-230 (in Korean). https://doi.org/10.5333/KGFS.2004.24.3.225
  7. Chu, C. C., C. C. Wang, C. S. Sun, C. Hsu, K. C. Yin, C. Y. Chu and F. Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scientia Sinic. 18:659-668.
  8. Elkonin, L. A. and N. V. Pakhomova. 1996. Influence of nitrogen sources on induction and growth of embryogenic callus in sorghum. Inter. Sorghum and Millets Newsletter 37:68-69.
  9. Gamborg, O. L., R. A. Miller and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151-158. https://doi.org/10.1016/0014-4827(68)90403-5
  10. Gupta, S., V. K. Khanna, R. Singh and G. K. Garg. 2006. Strategies for overcoming genotypic limitations of in vitro regeneration and determination of genetic components of variability of plant regeneration traits in sorghum. Plant Cell Tiss. Org. Cult. 86:379-388. https://doi.org/10.1007/s11240-006-9140-0
  11. Hagio, T. 2002. Adventitious shoot regeneration from immature embryos of sorghum. Plant Cell Tiss. Org. Cult. 68:65-72. https://doi.org/10.1023/A:1012918316140
  12. Han, F. G., H. Y. Zhao, F. Lin and L. G. Yang. 1997. Screening for salt tolerant lines through in vitro culture under salt stress conditions and studies on their different characters. Acta Agron. Sinica. 23:491-495.
  13. Jogeswar, G., D. Ranadheer, V. Anjaiah and P. B. Kavi Kishor. 2007. High Frequency somatic embryogenesis and regeneration in different genotypes of Sorghum bicolor (L.) Moench from immature inflorescence explants. In Vitro Cell. Develop. Biol. Plant 43:159-166.
  14. Kaeppler, H. F. and J. F. Pedersen. 1997. Evaluation of 41 elite and exotic inbred sorghum genotypes for high quality callus production. Plant Cell Tiss. Org. Cult. 48:71-75. https://doi.org/10.1023/A:1005765009568
  15. Maheswari, M., N. Lakshmi, S. K. Yadav, Y. Varalaxmi, A. V. Lakshmi, M. Vanaja and B. Venkateswarlu. 2006. Efficient plant regeneration from shoot apices of sorghum. Plant Biol. 50:741-744. https://doi.org/10.1007/s10535-006-0120-3
  16. Mamun, M. A., M. B. H. Sikdar, D. K. Paul, M. Rahman and R. Islam. 2004. In vitro micropropagation of some important sugarcane varieties of Bangladesh. Asian J. Plant Sci. 3:666-669. https://doi.org/10.3923/ajps.2004.666.669
  17. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Nahadi, S. and J. M. J. de-Wet. 1995. In vitro regeneration of Sorghum bicolor lines from shoot apexes. Inter. Sorghum and Millets Newsletter 36:88-90.
  19. Nguyen, Tuong-Van, T. T. Thanh, C. Martine and A. Geert. 2007. Agrobacterium-mediated transformation of sorghum (Sorghum bicolor (L.) Moench) using an improved in vitro regeneration system. Plant Cell Tiss. Org. Cult. 91:155-164. https://doi.org/10.1007/s11240-007-9228-1
  20. Oldach, K. H., A. Morgenstern, S. Rother, M. Girgi, O. M. Kennedy and H. Lorz. 2001. Efficient in vitro plant regeneration from immature zygotic embryos of pearl millet [Pennistum Glaucum (L.)R. Br.] and Sorghum bicolor (L.) Moench. Plant Cell Rep. 20:416-421. https://doi.org/10.1007/s002990100335
  21. O'Kennedy, M. M., A. Grootboom and P. R. Shewry. 2006. Harnessing sorghum and millet biotechnology for food and health. J. Cereal Sci. 44:224-235. https://doi.org/10.1016/j.jcs.2006.08.001
  22. Patil, V. M. and M. S. Kuruvinashetti. 1998. Plant regeneration from leaf sheath cultures of some rabi sorghum cultivars. South African J. Bot. 64:217-219. https://doi.org/10.1016/S0254-6299(15)30871-1
  23. Rao, K. V., P. Suprasanna and G. M. Reddy. 1990. Somatic embryogenesis from immature glume calli of Zea mays L. Indian J. Exp. Biol. 28:531-533.
  24. Shyamala, D. and P. Devi. 2003. Efficient regeneration of sorghum, Sorghum bicolor (L.) Moench, from shoot-tip explant. Indian J. Exp. Biol. 41:1482-1486.
  25. Vasil, V. and I. K. Vasil. 1981. Somatic embryogenesis and plant regeneration from tissue cultures of Pennisetum americanum, and P. americanum x P. purpureum hybrid. Ameri. J. Bot. 68:864-872. https://doi.org/10.2307/2443193
  26. Yohannes, T., L. Sagi, R. Swennen and M. Jacobs. 2003. Optimization of transformation conditions and production of transgenic sorghum (Sorghum bicolor) via microparticle bombardment. Plant Cell Tiss. Org. Cult. 75:1-18. https://doi.org/10.1023/A:1024664817800
  27. Zhao, Z., C. Tishu, T. Laura, M. Mike, W. Ning, P. Hong, R. Marjorie, S. Sheryl, H. Dave, S. Jon and P. Dortie. 2000. Agrobacterium-mediated sorghum transformation. Plant Mol. Biol. 44:789-798. https://doi.org/10.1023/A:1026507517182

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