The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Characterization and Immunopotentiating Effects of the Glycoprotein Isolated from Dioscorea Batatas

  • Huong, Pham Thi Thu (Department of Pharmacology, School of Medicine, Chosun University) ;
  • Lee, Chan-Ho (School of Pharmacy, Sungkyunkwan University) ;
  • Li, Mei-Hong (Department of Pharmacology, School of Medicine, Chosun University) ;
  • Lee, Min-Young (Department of Pharmacology, School of Medicine, Chosun University) ;
  • Kim, Joon-Ki (School of Pharmacy, Sungkyunkwan University) ;
  • Lee, Sun-Mee (School of Pharmacy, Sungkyunkwan University) ;
  • Seon, Jong-Heum (Department of Biotechnology and Biomedicine, Chungbuk Provincial College) ;
  • Lee, Dong-Cheol (Department of Biotechnology and Biomedicine, Chungbuk Provincial College) ;
  • Jeon, Young-Jin (Department of Pharmacology, School of Medicine, Chosun University)
  • Received : 2011.03.25
  • Accepted : 2011.04.18
  • Published : 2011.04.30
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Abstract

We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1${\beta}$, and TNF-${\alpha}$ in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1${\beta}$, and TNF-${\alpha}$, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.

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References

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