Journal of the Korean Society for Precision Engineering (한국정밀공학회지)

Korean Society for Precision Engineering (한국정밀공학회)
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Effect of Low Intensity Sound Wave on UC-MSC(Umbilical Cord Mesenchymal Stem Cell Growth

저강도 음파 조사가 중간엽 줄기세포 증식에 미치는 영향

  • Kim, Sung-Min (College of Biosystem Department of Medical Bio Engineering, Dongguk Univ.) ;
  • Kang, Seung-Ho (College of Biosystem Department of Medical Bio Engineering, Dongguk Univ.) ;
  • Jeong, Jae-Hoon (College of Biosystem Department of Medical Bio Engineering, Dongguk Univ.) ;
  • Park, Jung-Keug (College of Biosystem Department of Medical Bio Engineering, Dongguk Univ.) ;
  • Kim, Soo-Chan (Graduate School of Bio & Information Technology, Hankyong National Univ.)
  • 김성민 (동국대학교 의생명공학과) ;
  • 강승호 (동국대학교 의생명공학과) ;
  • 정재훈 (동국대학교 의생명공학과) ;
  • 박정극 (동국대학교 의생명공학과) ;
  • 김수찬 (한경대학교 생물정보통신전문대학원 IT 융합기술연구소)
  • Received : 2010.10.05
  • Accepted : 2011.01.17
  • Published : 2011.04.01
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Abstract

It is known that the low-intensity sound stimulation really affect to grow the cell. The cellular growth mechanism, however, does not been clearly identified even the effect on the low-intensity sound stimulation. The purpose of this study is to investigate the effect of low-intensity sound stimulation on the alveolar UC-MSC proliferation. Before the low-intensity sound stimulation is applied, the UC-MSC are cultured for 24 hours to facilitate their attachments. The cells are divided into two groups. And each was exposed to a medium with or without the low-intensity ultrasound stimulation at 71dB intensity level. The UC-MSC are again divided into three treatment groups of group 1, 2, and 3 and exposed to a frequency at 50Hz, 100Hz, and 1000Hz, respectively. In the results, it is investigated that the growth rates of UC-MSC for the stimulated groups were higher than those of control groups. In 1000Hz frequency, the number of UC-MSC cell is significantly higher than control groups (p>0.05). We would put the hypothesis that the cell growth could be enhanced by an appropriate low-intensity sound stimulation.

Keywords

References

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