한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제26권1호
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  • Pages.57-63
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  • 2011
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

소의 초기 임신 황체에서 PAPP-A와 $20{\alpha}$-HSD의 발현 양상

Expression of PAPP-A and $20{\alpha}$-HSD in the Bovine Corpus Luteum during Early Pregnancy

  • 김대승 (국립한경대학교 생물환경정보통신전문대학원 동물생명공학) ;
  • 김상환 (국립한경대학교 생물환경정보통신전문대학원 동물생명공학) ;
  • 윤종택 (국립한경대학교 유전공학연구소)
  • Kim, Dae-Seung (Department of Animal Life Science, Hankyong National University) ;
  • Kim, Sang-Hwan (Department of Animal Life Science, Hankyong National University) ;
  • Yoon, Jong-Taek (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2011.02.24
  • 심사 : 2011.03.04
  • 발행 : 2011.03.31
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초록

This study was performed to the expressions of pregnancy-associated plasma protein-A (PAPP-A) and 20alpha-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) in bovine corpus luteum during early pregnancy. To determine the function of PAPP-A gene during early pregnancy, we collected corpus luteum samples on 30, 60 and 90 days of pregnancy in bovine. The mRNA expression of PAPP-A, $20{\alpha}$-HSD, progesterone-receptor (PR) and insulin-like growth factor binding protein4 (IGFBP4) gene was conducted by Real-time PCR. In parallel with mRNA levels, The protein expressions of PAPP-A and $20{\alpha}$-HSD were detected by immunological analysis. The mRNA expressions $20{\alpha}$-HSD and PAPP-A significantly increased on day 90 in the corpus luteum during pregnancy. The mRNA expression of PR and JGFBP4 in the corpus luteum progressively was enhanced at 30 to 60 day, but decreased on 90 day of pregnancy in the corpus luteum. The expression patterns of these genes, PAPP-A and $20{\alpha}$-HSD were similar pattern in these tissues. In conclusion, PAPP-A and $20{\alpha}$-HSD activity in corpus luteum could be played a role for early pregnancy manifestation.

키워드

참고문헌

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