Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Practical Procedure of Sperm Cryopreservation of the Bar-tailed Flathead Platycephalus indicus

양태, Platycephalus indicus의 정자의 냉동보존

  • Kim, Do-Hyung (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University) ;
  • Kong, Il-Keun (Department of Animal Science, College of Agriculture and Life Science, Gyeongsang National University) ;
  • Rha, Sung-Ju (Fisheries Science Institute, Chonnam National University) ;
  • Yun, Ji-Won (Fisheries Science Institute, Chonnam National University) ;
  • Han, Kyeong-Ho (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University) ;
  • Kho, Kang-Hee (Department of Aquatic Biology, College of Fisheries and Ocean Sciences, Chonnam National University)
  • 김도형 (전남대학교 수산해양대학 양식생물학과) ;
  • 공일근 (경상대학교 농업생명과학대학 축산학과) ;
  • 라성주 (전남대학교 수산과학연구소) ;
  • 윤지원 (전남대학교 수산과학연구소) ;
  • 한경호 (전남대학교 수산해양대학 양식생물학과) ;
  • 고강희 (전남대학교 수산해양대학 양식생물학과)
  • Received : 2011.01.15
  • Accepted : 2011.03.25
  • Published : 2011.03.31
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Abstract

This study was conducted to investigate protocol standardization for cryopreservation spermatozoa of the bar-tailed flathead Platycephalus indicus. The suitability of the cryoprotectants, dimethyl sulphoxide (DMSO), glycerol and methanol were tested against three freezing rates and three thawing temperatures. DMSO and glycerol gave significantly higher motile index and survival rates than methanol. Among the freezing rates, freezing at a height of 2 cm above $LN_2$ surface for $10\;min^{-1}$ gave higher motile index and survival rates. In terms of best thawing temperature, $20^{\circ}C$ obtained the highest motility.

양태, Platycephalus indicus 정자의 동결보존을 위해 적정 동해방지제와 동결높이에 따른 예비동결(0, 2, 4, 6cm), 그리고 해동온도에 따른 정자의 운동성과 생존율로 비교하였다. 적정 동해방지제로 dimethylsulphoxide(DMSO), glycerol, methanol에 대해 실험하였으며, DMSO와 glycerol에서 methanol에 비하여 높은 생존율과 운동성을 나타내었다. 또한, 동결보존에 있어 예비동결은 액체질소표면으로부터 2 cm 높이에서 높은 생존율과 운동성을 보였고, 해동온도로는 $20^{\circ}C$가 가장 적합하였다.

Keywords

References

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