한국안광학회지 (Journal of Korean Ophthalmic Optics Society)

한국안광학회 (The Korean Ophthalmic Optics Society)
권호 표지

Rhodamine B 염료의 토끼 망막 뮬러세포에 대한 선택적 염색

Selective Accumulation of Rhodamine B in Müller cells in Rabbit Retina

  • 권오주 (부산정보대학 보건웰빙학부 안경광학과) ;
  • 이은실 (경북대학교 자연과학대학 생물학과) ;
  • 전창진 (경북대학교 자연과학대학 생물학과)
  • Kwon, Oh Ju (Department of Ophthalmic Optics, Busan College of Information Technology) ;
  • Lee, Eun Shil (Department of Biology, College of Natural Science, Kyungpook National University) ;
  • Jeon, Chang Jin (Department of Biology, College of Natural Science, Kyungpook National University)
  • 투고 : 2010.07.26
  • 심사 : 2011.03.19
  • 발행 : 2011.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

목적: 본 연구는 토끼 망막에서 특정 신경 세포 염색이 가능한 염료를 조사하였다. 방법: Rhodamine B를 토끼의 안구내 유리체강에 주입하였다. 24시간 후, 망막을 분리하여 현미경으로 염색 상태를 관찰하였다. 염색 된 세포는 그 종류를 알아보기 위하여 면역조직화학적방법을 실시하였다. 결과: 망막 내핵층 중간 위치에서 선명하게 염색된 핵들이 관찰되었다. 염색된 세포의 분포도와 수가 뮬러세포와 비슷한 양상을 보였다. 뮬러세포인지 확인하기 위하여 vimentin 항체를 사용하였다. Rhodamine B에 의해 염색된 핵들은 vimentin 항체로 감싸져 있어 뮬러세포임을 확인하였다. 결론: Rhodamine B를 살아있는 망막에 넣을 경우 토끼 망막에 신경교세포의 특이적 염색이 나타났다.

Purpose: In this study, we investigated the dye to staining for selective accumulation in rabbit retina. Methods: Rhodamine B was injected into the vitreous body in rabbit. After 24 h, the isolated retina was checked condition of cell staining on the microscope. We used conventional immunocytochemical techniques for recognizing cell type. Results: Well-labeled nuclei were seen in the middle of the inner nuclear layer of the rabbit retina. The number and distrbution of the accumulating cells were similar to those of the m$\ddot{u}$ller glia. To identify m$\ddot{u}$ller cell, we used antibody directed against vimentin. Rhodamine B-immunoreactive nuclei also were labeled with antivimentin antibody. We found that Rhodamine B was accumulated selectively in retinal m$\ddot{u}$ller cell. Conclusions: Specific accumulation in rabbit retinal m$\ddot{u}$ller cell occurred when Rhodamine B was applied to living retina.

키워드

참고문헌

  1. Lafarga M., Casafont I., Bengoechea R., Tapia O., and Berciano M. T., "Cajal's contribution to the knowledge of the neuronal cell nucleus", Chromosoma., 118(4):437-443(2009). https://doi.org/10.1007/s00412-009-0212-x
  2. Rodieck R. W., "The first steps in seeing", Sunderland. MA. Sinauer., pp. 119-120(1998).
  3. Yang G. and Masland R. H., "Direct visualization of the dendritic and receptive fields of directionally selective retinal ganglion cells", Science, 258(5090):1949-1952(1992). https://doi.org/10.1126/science.1470920
  4. Brecha N., "A review of retinal neurotransmitters: Histochemical and biochemical studies. In: Chemical Neuroanatomy (P.C. Emson, Ed.)", Raven Press, New York, pp. 85-129(1983).
  5. Masland R. H., "The functional architecture of the retina", Sci. Am., 255(6):102-111(1986). https://doi.org/10.1038/scientificamerican1286-102
  6. Vaney D. I., "Many diverse types of retinal neurons show tracer coupling when injected with biocytin or Neurobiotin", Neurosci. Lett., 125(2):187-190(1991). https://doi.org/10.1016/0304-3940(91)90024-N
  7. Tauchi M. and Masland R. H., "The shape and arrangement of the cholinergic neurons in the rabbit retina", Proc. R. Soc. Lond. B. Biol. Sci., 223(1230):101-119(1984). https://doi.org/10.1098/rspb.1984.0085
  8. Sandell J. H. and Masland R. H., "A system of indoleamine-accumulating neurons in the rabbit retina", J. Neurosci., 6(11):3331-3347(1986).
  9. Sandell J. H. and Masland R. H., "Shape and distribution of an unusual retinal neuron", J. Comp. Neurol., 280(3):489-497(1989). https://doi.org/10.1002/cne.902800312
  10. Vaney D. I., "Morphological identification of serotoninaccumulating neurons in the living retina", Science, 233(4762):444-446(1986). https://doi.org/10.1126/science.3726538
  11. Masland R. H., "Amacrine cells", Trends. Neurosci., 11(9):405-10(1988). https://doi.org/10.1016/0166-2236(88)90078-1
  12. Jeon C. J. and Masland R. H., "Selective accumulation of diamidino yellow and chromomycin A3 by retinal glial cells", J. Histochem. Cytochem, 41(11):1651-1658(1993). https://doi.org/10.1177/41.11.8409373
  13. Thanos S., Vidal-Sanz M., and Aguayo A. J., "The use of rhodamin-B-isothicyanate (RITC) as an anterograde and retrograde tracer in the adult rat visual system", Brain. Res., 406(1-2):317-321(1987).
  14. Lincoln V. J., Marcia L. W., and Lan B. C., "Localization of mitochondria in living cells with rhodamine 123", Cell. Biology, 77(2):990-994(1980).
  15. Mark F. B., Barry W. C., and Michael A. P., "Neuroscience", Lippincott Williams & Wilkins, (2001).
  16. Rideta J. L., Privata A., Malhotrab S. K., and Gagec F. H., "Reactive astrocytes: cellular and molecular cues to biological function", Trends in Neurosciences, 20(12):570-577(1997). https://doi.org/10.1016/S0166-2236(97)01139-9
  17. Unger J. W., "Glial reaction in aging and Alzheimer's disease", Microsc. Res. Tech., 43(1):24-28(1998). https://doi.org/10.1002/(SICI)1097-0029(19981001)43:1<24::AID-JEMT4>3.0.CO;2-P
  18. Wilson J. X., "Antioxidant defense of the brain: a role for astrocytes", Can. J. Physiol. Pharmacol., 75(10-11):1149-1163(1997). https://doi.org/10.1139/y97-146
  19. Humphrey M. F., Constable I. J., Chu Y., and Wiffen S., "A quantitative study of the lateral spread of Muller cell responses to retinal lesions in the rabbit", J. Comp. Neurol., 334(4):545-558(1993). https://doi.org/10.1002/cne.903340404
  20. Michael A. D. and Constance L. C., "Control of muller glial cell proliferation and activation following retinal injury", Nature neuroscience, 3(9):873-880(2000). https://doi.org/10.1038/78774
  21. Atsushi K., Yasumasa O., and Colin J. B., "Muller cell protection of rat retinal ganglion cells from glutamate and nitric oxide neurotoxicity", I.O.V.S., 41(11):3444-3450(2000).
  22. Uckermann O., Iandiev I., Francke M., Franze K., Grosche J., Wolf S., et al., "Selective staining by vital dyes of Muller glial cells in retinal wholemounts", lia., 45(1):59-66(2004).
  23. 김재민, 김상문, 김창식, "시기해부학", 현문사, 서울, pp. 89(2000).