Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Effects of Balneotherapy in Alkaline Reduced Spring Water on Skin Injury Induced by UV Irradiation in Hairless Mice

자외선으로 손상된 무모생쥐의 피부에 알칼리환원온천수의 침수치료효과

  • Yoon, Yang-Suk (Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine) ;
  • Kim, Dong-Heui (Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine) ;
  • Jin, Dan (Department of Immunology and Pathogenic Biology, College of Medicine, Yanbian University) ;
  • Park, Mi-Soon (Seoul Medical Science Institute) ;
  • Chang, Byung-Soo (Department of Cosmetology, Hanseo University) ;
  • Lee, Jee-Yeon (Department of Cosmetology, Hanseo University) ;
  • Lee, Kyu-Jae (Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine)
  • 윤양숙 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김동희 (연세대학교 원주의과대학 환경의생물학교실) ;
  • 김단 (연변대학교 의과대학 면역병리생물학교실) ;
  • 박미순 (서울의과학연구소) ;
  • 장병수 (한서대학교 보건학부 피부미용학과) ;
  • 이지연 (한서대학교 보건학부 피부미용학과) ;
  • 이규재 (연세대학교 원주의과대학 환경의생물학교실)
  • Received : 2011.02.14
  • Accepted : 2011.03.22
  • Published : 2011.03.31
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Abstract

Balneotherapy has been widely used for the treatment of skin diseases in the world. The purpose of this study was to determine the bath effect of the alkaline reduced spring water with the properties of high pH and low oxidation reduction potential (ORP) on the skin injury induced by ultra violet (UV) irradiation. For this purpose, hairless mice were irradiated with UV-B to cause skin injury, and individually taken a bath in spring water (experimental group) and tap water (control group) once a day for 40 min during 21 days. We observed histological changes of the back skin through macro- and microscopic methods compared to the control group. We found that skin injury of the experimental group was more quickly recovered than that of the control group. Under the light microscope, the experimental group showed that epidermal thickening (p<0.01) and the mast cell activation (p<0.001) were lower compared with the control group, in addition infiltration of inflammatory cells and degranulation of mast cells were less observed. These results suggest that regular bath in the spring water with the properties of high pH and low ORP has a positive effect on the skin injury induced by UV irradiation.

피부질환의 개선을 위한 수치료요법은 예로부터 세계 각지에서 널리 이용되어 왔다. 본 연구에서는 국내 알칼리환원온천수를 이용한 수치료요법이 손상된 피부에 어떠한 영향을 미치는지 확인하고자 하였다. 무모생쥐(hairless mice)의 등 부위에 자외선을 조사하여 피부 손상을 인위적으로 유발한 후 매일 실온에서 40분간 침적시켜 21일간 수치료를 실시한 후 생쥐의 피부조직을 분리하여 검경하였다. 수치료를 하는 동안 실험군의 피부 회복속도가 대조군에 비해 더 빠른 경향으로 관찰되었다. 조직학적으로 관찰한 결과, 대조군에 비해 실험군에서 표피층 비후 및 염증세포 침착 현상이 상대적으로 적게 나타났으며, 비만세포의 증가 및 탈과립 현상 또한 적게 나타났다. 이는 온천수에 함유된 황성분의 박피효과 및 항균작용과 함께 온천수의 항산화능이 피부조직의 치유에 도움을 준 것으로 사료된다.

Keywords

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