KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Antibacterial Activity of Actinomycetes Producing Growth Inhibition Compounds Against Multi-antibiotic Resistant Pseudomonas Aeruginosa

항생제 다제내성균 Pseudomonas aeruginosa에 대한 생육저해물질 생산 방선균의 분리 및 항균활성

  • Kang, Dong-Hee (Department of Microbiology, College of Natural Science, Keimyung University) ;
  • Bae, Ho-Kyung (Department of Microbiology, College of Natural Science, Keimyung University) ;
  • Kim, Hyun-Soo (Department of Microbiology, College of Natural Science, Keimyung University)
  • 강동희 (계명대학교 자연과학대학 미생물학과) ;
  • 배호경 (계명대학교 자연과학대학 미생물학과) ;
  • 김현수 (계명대학교 자연과학대학 미생물학과)
  • Received : 2010.10.26
  • Accepted : 2011.02.24
  • Published : 2011.02.28
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Abstract

Of the 500 Actinomycetes isolates obtained from soil, one isolate grown on maltose as the sole carbon source produced compound BHK-P19, which inhibited the growth of multiple drug resistant P. aeruginosa 0245. Ultraviolet radiation mutagenesis curtailed production of BHK-P19. Mutation of the BHK-P19 producer using N-methyl-N'-nitro-N-nitroso-guanidine obviated the antibacterial activity to P. aeruginosa 0245, but not towards P. aeruginosa 0225. The mixing of BHK-P19 and BHK-S5 culture extracts inhibited P. aeruginosa 0254, 0225 and 1113. The combined application of BHK-P19 culture extract and Schizandra chinensis Baillon extract inhibited P. aeruginosa 0254, 0225, 0826, 1113, 1378, 1731 and 2492. Use of various concentrations of BHK-P19 culture extract and ampicillin markedly increased antibacterial activity against multi-drug resistant P. aeruginose 1113.

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References

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