Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

Simultaneous Determination of Eight Sugar Alcohols in Foodstuffs by High Performance Liquid Chromatography

HPLC를 이용한 식품 중 당알코올 8종 동시분석

  • Lim, Ho-Soo (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Park, Sung-Kwan (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Kwak, In-Shin (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Kim, Hyung-Il (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Sung, Jun-Hyun (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration) ;
  • Choi, Jung-Yoon (Korea Food Research Institute, Korea Foods Industry Association) ;
  • Kim, So-Hee (Food Additives and Packages Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration)
  • 임호수 (식품의약품안전청 식품의약품안전평가원 첨가물포장과) ;
  • 박성관 (식품의약품안전청 식품의약품안전평가원 첨가물포장과) ;
  • 곽인신 (식품의약품안전청 식품의약품안전평가원 첨가물포장과) ;
  • 김형일 (식품의약품안전청 식품의약품안전평가원 첨가물포장과) ;
  • 성준현 (식품의약품안전청 식품의약품안전평가원 첨가물포장과) ;
  • 최정윤 (한국식품공업협회 부설 식품연구소) ;
  • 김소희 (식품의약품안전청 식품의약품안전평가원 첨가물포장)
  • Received : 2010.11.05
  • Accepted : 2011.02.08
  • Published : 2011.03.31
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Abstract

A method was established for the simultaneous determination of sugar alcohols, erythritol, xylitol, sorbitol, inositol, mannitol, maltitiol, lactitol and isomalt by High Performance Liquid Chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride (PNBC). HPLC was performed on Imtakt Unison US-$C_18$ column, using acetonitrile: water (77:23) as a mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10~200 mg/L range. The average recoveries of the sugar alcohols from three confectioneries spiked at 100 ppm of eight sugar alcohol standards ranged from 81.2 to 123.1% with relative standard deviations ranging fromo 0.2 to 4.9%. The limits of detection (LODs) were $0.5{\sim}8\;{\mu}g/L$ and the limits of quantification (LOQs) were $2{\sim}17\;{\mu}g/L$. Reproducibility of 8 sugar alcohols was 0.28~1.97 %RSD. The results of the analysis of confectioneries showed that 89 samples of 130 were detected and the sugar alcohols content of samples investigated varied between 0.4 and 693.7 g/kg. A method for the simultaneous determination of eight sugar alcohols will be used as basic data for control of sugar alcohols in confectioneries, and quality control in food manufacturing.

본 연구에서는 고속액체크로마토그래프를 이용하여 당알콜 즉, 에리스리톨, 자일리톨, 소르비툴, 이노시툴, 만니톨, 말티톨, 락티툴, 이소말트의 동시분석법을 확립하였다. 당알콜은 p-nitribenzoyl chloride (PNBC)을 사용하여 자외선을 강하게 흡수하는 유도체로 전환되었다. HPLC는 이동상으로는 아세토니트릴 : 물(77:23), UV 검출(260 nm) 및 Imtakt Unison US-$C_18$ 컬럼을 이용하여 수행되었다. 모든 당알콜의 표준곡선은 10~200 mg/L 범위에서 직선성을 보였다. 당알콜 8종을 100 ppm이 되도록 spiking한 3가지 유형의 가공식품에 대해 당알콜의 평균 회수율은 81.2~123.1%(상대표준편자 0.2~4.9%)이었다. 검출한계 (LOD)는 $0.5{\sim}8\;{\mu}g/L$이었으며, 정량한계는(LOQ)는 $2{\sim}17\;{\mu}g/L$이었다. 당알콜의 재현성은 0.28~1.97 %RSD이었다. 국내유통 가공식품 중 당알콜 분석결과 총 130건 중 89건이 검출되었으며, 검출범위는 0.4~693.7 g/kg이었다. 본 연구를 통하여 확립된 식품 중 당알코올 동시분석법은 가공식품에 대한 당알코올의 사전 사후관리 및 식품 제조시 품질관리를 위한 기초자료로 활용될 것으로 본다.

Keywords

Acknowledgement

Supported by : 식품의약품안전청

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