Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Effects of Dilute Acid Pretreatment on Enzyme Adsorption and Surface Morphology of Liriodendron tulipifera

  • Min, Byeong-Cheol (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Koo, Bon-Wook (Department of Wood and Paper Science, College of Natural Resource, 2105 Biltmore Hall, North Carolina State University) ;
  • Gwak, Ki-Seob (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Yeo, Hwan-Myeong (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Joon-Weon (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, In-Gyu (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2011.02.28
  • Accepted : 2011.03.14
  • Published : 2011.03.25
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Abstract

In this study, dilute acid pretreatment of $Liriodendron$ $tulipifera$ was performed for enzymatic hydrolysis. As the pretreatment temperature was increased, enzymatic hydrolysis and enzyme adsorption yield also increased. The highest enzymatic hydrolysis yield was 57% (g/g) and enzyme adsorption was 44% (g/g). Enzymatic hydrolysis yield was determined with weight loss of pretreated biomass by enzyme, and enzyme adsorption was a percentage of enzyme weight attaching on pretreated biomass compared with input enzyme weight. When $L.$ $tulipifera$ was pretreated with 1% sulfuric acid at $160^{\circ}C$ for 5 min., hemicellulose was significantly removed in pretreatment, but the lignin contents were constant. Other changes in surface morphology were detected on biomass pretreated at $160^{\circ}C$ by a field emission scanning electron microscope (FESEM). A large number of spherical shapes known as lignin droplets were observed over the entire biomass surface after pretreatment. Hemicellulose removal and morphological changes improved enzyme accessibility to cellulose by increasing cellulose exposure to enzyme. It is thus evidence that enzyme adsorption is a significant factor to understand pretreatment effectiveness.

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