The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Activity of 1-docosanoyl Cafferate Isolated from Rhus verniciflua in LPS-stimulated BV2 Microglial Cells

  • Lee, Jae-Won (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Cheong, Il-Young (Department of Anesthesiology, College of Medicine, Kangwon National University) ;
  • Kim, Hae-Sung (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Jae-Jun (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Yong-Suk (Department of Anesthesiology, College of Medicine, Kangwon National University) ;
  • Kwon, Yong-Soo (College of Pharmacy, Kangwon National University) ;
  • Kim, Myong-Jo (Division of Bio-resources Technology, Kangwon National University) ;
  • Lee, Hee-Jae (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Chun, Wan-Joo (Department of Pharmacology, College of Medicine, Kangwon National University)
  • Received : 2010.11.19
  • Accepted : 2011.01.17
  • Published : 2011.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Although various derivatives of caffeic acid have been reported to possess a wide variety of biological activities such as protection of neuronal cells against excitotoxicity, the biological activity of 1-docosanoyl cafferate (DC) has not been examined. The objective of the present study was to evaluate the anti-inflammatory effects of DC, isolated from the stem bark of Rhus verniciflua, on lipopoly-saccharide (LPS)-stimulated BV2 microglial cells. Pretreatment of cells with DC significantly attenuated LPS-induced NO production, and mRNA and protein expression of iNOS in a concentration-dependent manner. DC also significantly suppressed LPS-induced release of cytokines such as TNF-${\alpha}$ and IL-$1{\beta}$. Consistent with the decrease in cytokine release, DC dose-dependently and significantly attenuated LPS-induced mRNA expression of these cytokines. Furthermore, DC significantly suppressed LPS-induced degradation of IKB, which retains NF-kB in the cytoplasm. Therefore, nuclear translocation of NF-kB induced by LPS stimulation was significantly suppressed with DC pretreatment. Taken together, the present study suggests that DC exerts its anti-inflammatory activity through the suppression of NF-kB translocation to the nucleus.

Keywords

References

  1. Perry VH, Gordon S. Macrophages and microglia in the nervous system. Trends Neurosci. 1988;11:273-277. https://doi.org/10.1016/0166-2236(88)90110-5
  2. Hailer NP. Immunosuppression after traumatic or ischemic CNS damage: it is neuroprotective and illuminates the role of microglial cells. Prog Neurobiol. 2008;84:211-233. https://doi.org/10.1016/j.pneurobio.2007.12.001
  3. Matsumoto H. Some markers reflecting the pathology and disease activity of multiple sclerosis. No To Shinkei. 1992;44: 95-102.
  4. McGeer PL, McGeer EG. Glial cell reactions in neurodegenerative diseases: pathophysiology and therapeutic interventions. Alzheimer Dis Assoc Disord. 1998;12 Suppl 2:S1-6. https://doi.org/10.1097/00002093-199803000-00001
  5. Itagaki S, McGeer PL, Akiyama H, Zhu S, Selkoe D. Relationship of microglia and astrocytes to amyloid deposits of Alzheimer disease. J Neuroimmunol. 1989;24:173-182. https://doi.org/10.1016/0165-5728(89)90115-X
  6. Merrill JE, Chen IS. HIV-1, macrophages, glial cells, and cytokines in AIDS nervous system disease. FASEB J. 1991;5: 2391-2397.
  7. Chao CC, Hu S, Close K, Choi CS, Molitor TW, Novick WJ, Peterson PK. Cytokine release from microglia: differential inhibition by pentoxifylline and dexamethasone. J Infect Dis. 1992;166:847-853. https://doi.org/10.1093/infdis/166.4.847
  8. Graeber MB, Streit WJ. Microglia: biology and pathology. Acta Neuropathol. 2010;119:89-105. https://doi.org/10.1007/s00401-009-0622-0
  9. Merrill JE, Benveniste EN. Cytokines in inflammatory brain lesions: helpful and harmful. Trends Neurosci. 1996;19:331-338. https://doi.org/10.1016/0166-2236(96)10047-3
  10. Tuttolomondo A, Di Raimondo D, di Sciacca R, Pinto A, Licata G. Inflammatory cytokines in acute ischemic stroke. Curr Pharm Des. 2008;14:3574-3589. https://doi.org/10.2174/138161208786848739
  11. Kim MJ, Choi WC, Barshinikov AM, Koba-yashi A. Anticancer and antioxidant activity of allergen-removed extract in Rhus verniciflua stokes. Korean J Med Crop Sci. 2002;10:288- 293.
  12. Kim ML, Choi YH, Kim WG, Kwak SS. Antioxidative activity of urushiol derivatives from the sap oflacquer tree (Rhus verniciflua Stokes). Korean J Plant Resour. 1997;10:227-230.
  13. Kim MJ, Kim CJ, Kwak SS. Antifungal activity of urushiol component in the sap of Korean lacquer tree (Rhus vernicifera Stokes). Korean J Plant Resour. 1997;10:231-234.
  14. Park KY, Jung GO, Lee KT, Choi J, Choi MY, Kim GT, Jung HJ, Park HJ. Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J Ethnopharmacol. 2004;90:73-79. https://doi.org/10.1016/j.jep.2003.09.043
  15. Jeong JS,Park JW, Yoon SW. Carcinostatic effect of allergen removed Rhus verniciflua Stokes based traditional Korean medicine on a patient with lung adenocarcinoma; single case report. Orient Pharm Exp Med. 2008;7:573-578. https://doi.org/10.3742/OPEM.2008.7.5.573
  16. Jeon WK, Lee JH, Kim HK, Lee AY, Lee SO, Kim YS, Ryu SY, Kim SY, Lee YJ, Ko BS. Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes. J Ethnopharmacol. 2006;106:62-69. https://doi.org/10.1016/j.jep.2005.12.015
  17. Byun JS, Han YH, Hong SJ, Hwang SM, Kwon YS, Lee HJ, Kim SS, Kim MJ, Chun W. Bark constituents from mushroomdetoxified Rhus verniciflua suppress kainic acid-induced neuronal cell death in mouse hippocampus. Korean J Physiol Pharmacol. 2010;14:279-283. https://doi.org/10.4196/kjpp.2010.14.5.279
  18. Kim SR, Kim YC. Neuroprotective phenylpropanoid esters of rhamnose isolated from roots of Scrophularia buergeriana. Phytochemistry. 2000;54:503-509. https://doi.org/10.1016/S0031-9422(00)00110-2
  19. Sul D, Kim HS, Lee D, Joo SS, Hwang KW, Park SY. Protective effect of caffeic acid against beta-amyloid-induced neurotoxicity by the inhibition of calcium influx and tau phosphorylation. Life Sci. 2009;84:257-262. https://doi.org/10.1016/j.lfs.2008.12.001
  20. Kim YC. Neuroprotective phenolics in medicinal plants. Arch Pharm Res. 2010;33:1611-1632. https://doi.org/10.1007/s12272-010-1011-x
  21. Wei X, Ma Z, Fontanilla CV, Zhao L, Xu ZC, Taggliabraci V, Johnstone BH, Dodel RC, Farlow MR, Du Y. Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity. Neuroscience. 2008; 155:1098-1105. https://doi.org/10.1016/j.neuroscience.2008.06.056
  22. Tsai SK, Lin MJ, Liao PH, Yang CY, Lin SM, Liu SM, Lin RH, Chih CL, Huang SS. Caffeic acid phenethyl ester ameliorates cerebral infarction in rats subjected to focal cerebral ischemia. Life Sci. 2006;78:2758-2762. https://doi.org/10.1016/j.lfs.2005.10.017
  23. Komatsu M, Tomimori T, Hatayama K, Makiguchi Y. Studies on the constituents of Sophora species. 3. Constituents of the root of Sophora subprostrata Chun et T. Chen. Yakugaku Zasshi. 1970;90:459-462. https://doi.org/10.1248/yakushi1947.90.4_459
  24. Ghosh S, May MJ, Kopp EB. NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol. 1998;16:225-260. https://doi.org/10.1146/annurev.immunol.16.1.225
  25. Bonizzi G, Karin M. The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol. 2004;25:280-288. https://doi.org/10.1016/j.it.2004.03.008
  26. Lapchak PA. The phenylpropanoid micronutrient chlorogenic acid improves clinical rating scores in rabbits following multiple infarct ischemic strokes: synergism with tissue plasminogen activator. Exp Neurol. 2007;205:407-413. https://doi.org/10.1016/j.expneurol.2007.02.017
  27. Li YY, Lu JH, Li Q, Zhao YY, Pu XP. Pedicularioside A from Buddleia lindleyana inhibits cell death induced by 1-methyl- 4-phenylpyridinium ions (MPP+) in primary cultures of rat mesencephalic neurons. Eur J Pharmacol. 2008;579:134-140. https://doi.org/10.1016/j.ejphar.2007.10.052
  28. Jiang WL, Tian JW, Fu FH, Zhu HB, Hou J. Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury. Eur J Pharmacol. 2010;640:75-81. https://doi.org/10.1016/j.ejphar.2010.04.055
  29. Deng M, Zhao JY, Tu PF, Jiang Y, Li ZB, Wang YH. Echinacoside rescues the SHSY5Y neuronal cells from TNFalpha-induced apoptosis. Eur J Pharmacol. 2004;505:11- 18. https://doi.org/10.1016/j.ejphar.2004.09.059
  30. Kang SS, Kim CM. Studies on the Korean Indigenous Plants. Isolation of 1-eicosanoyl cafferate from Echinosophora koreensis. Arch Pharm Res. 1987;10:67-68. https://doi.org/10.1007/BF02855623
  31. Kreutzberg GW. Microglia: a sensor for pathological events in the CNS. Trends Neurosci. 1996;19:312-318. https://doi.org/10.1016/0166-2236(96)10049-7
  32. Aquilano K, Baldelli S, Rotilio G, Ciriolo MR. Role of nitric oxide synthases in Parkinson's disease: a review on the antioxidant and anti-inflammatory activity of polyphenols. Neurochem Res. 2008;33:2416-2426. https://doi.org/10.1007/s11064-008-9697-6
  33. Hashioka S, McGeer PL, Monji A, Kanba S. Anti-inflammatory effects of antidepressants: possibilities for preventives against Alzheimer's disease. Cent Nerv Syst Agents Med Chem. 2009;9: 12-19. https://doi.org/10.2174/187152409787601897
  34. Ray B, Lahiri DK. Neuroinflammation in Alzheimer's disease: different molecular targets and potential therapeutic agents including curcumin. Curr Opin Pharmacol. 2009;9:434-444. https://doi.org/10.1016/j.coph.2009.06.012
  35. Li Q, Verma IM. NF-kappaB regulation in the immune system. Nat Rev Immunol. 2002;2:725-734. https://doi.org/10.1038/nri910
  36. Karin M, Takahashi T, Kapahi P, Delhase M, Chen Y, Makris C, Rothwarf D, Baud V, Natoli G, Guido F, Li N. Oxidative stress and gene expression: the AP-1 and NF-kappaB connections. Biofactors. 2001;15:87-89. https://doi.org/10.1002/biof.5520150207
  37. Cho IH, Hong J, Suh EC, Kim JH, Lee H, Lee JE, Lee S, Kim CH, Kim DW, Jo EK, Lee KE, Karin M, Lee SJ. Role of microglial IKKbeta in kainic acid-induced hippocampal neuronal cell death. Brain. 2008;131:3019-3033. https://doi.org/10.1093/brain/awn230
  38. Romano MF, Avellino R, Petrella A, Bisogni R, Romano S, Venuta S. Rapamycin inhibits doxorubicin-induced NF-kappaB/ Rel nuclear activity and enhances the apoptosis of melanoma cells. Eur J Cancer. 2004;40:2829-2836. https://doi.org/10.1016/j.ejca.2004.08.017
  39. Barger SW, Horster D, Furukawa K, Goodman Y, Krieglstein J, Mattson MP. Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and $Ca^{2＋}$ accumulation. Proc Natl Acad Sci U S A. 1995;92:9328-9332. https://doi.org/10.1073/pnas.92.20.9328
  40. Goodman Y, Mattson MP. Ceramide protects hippocampal neurons against excitotoxic and oxidative insults, and amyloid beta-peptide toxicity. J Neurochem. 1996;66:869-872.
  41. Mattson MP, Goodman Y, Luo H, Fu W, Furukawa K. Activation of NF-kappaB protects hippocampal neurons against oxidative stress-induced apoptosis: evidence for induction of manganese superoxide dismutase and suppression of peroxynitrite production and protein tyrosine nitration. J Neurosci Res. 1997;49:681-697. https://doi.org/10.1002/(SICI)1097-4547(19970915)49:6<681::AID-JNR3>3.0.CO;2-3

Cited by

  1. Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells vol.19, pp.4, 2011, https://doi.org/10.4062/biomolther.2011.19.4.431
  2. 3,4,5-Trihydroxycinnamic acid inhibits lipopolysaccharide (LPS)-induced inflammation by Nrf2 activation in vitro and improves survival of mice in LPS-induced endotoxemia model in vivo vol.390, pp.1, 2014, https://doi.org/10.1007/s11010-014-1965-y
  3. 3,4,5-Trihydroxycinnamic acid inhibits lipopolysaccharide (LPS)-induced inflammation by Nrf2 activation in vitro and improves survival of mice in LPS-induced endotoxemia model in vivo vol.390, pp.1, 2014, https://doi.org/10.1007/s11010-014-1965-y
  4. 3,4,5-Trihydroxycinnamic acid increases heme-oxygenase-1 (HO-1) and decreases macrophage infiltration in LPS-induced septic kidney vol.397, pp.1, 2014, https://doi.org/10.1007/s11010-014-2177-1
  5. Shikonin inhibits TNF-α production through suppressing PKC-NF-κB-dependent decrease of IL-10 in rheumatoid arthritis-like cell model vol.71, pp.2, 2011, https://doi.org/10.1007/s11418-016-1064-3
  6. 3,4,5-Trihydroxycinnamic Acid Inhibits LPS-Induced Inflammatory Response by Increasing SIRT1 Expression in Human Umbilical Vein Endothelial Cells vol.57, pp.5, 2011, https://doi.org/10.1159/000507628
  7. Noni Inhibits Neuronal Damage Caused by the Immune Reaction of Microglial Cells Activated by Doxorubicin vol.52, pp.4, 2011, https://doi.org/10.15324/kjcls.2020.52.4.389