The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Effects of Angelicae Pubescentis Radix Water Extract on Immune Property in RAW 264.7 Macrophages

독활 물 추출물이 대식세포 면역 활성에 미치는 영향

  • Lee, Jong-Han (Dept. of Gynecology, College of Oriental Medicine, Kyungwon University) ;
  • Kim, Yoon-Sang (Dept. of Gynecology, College of Oriental Medicine, Kyungwon University) ;
  • Lim, Eun-Mee (Dept. of Gynecology, College of Oriental Medicine, Kyungwon University)
  • 이종한 (경원대학교 한의과대학 부인과학교실) ;
  • 김윤상 (경원대학교 한의과대학 부인과학교실) ;
  • 임은미 (경원대학교 한의과대학 부인과학교실)
  • Received : 2010.12.20
  • Accepted : 2010.12.30
  • Published : 2011.01.30
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Abstract

Objectives: The purpose of this study was to investigate the effects of Angelicae pubescentis Radix water extract (ACE) on immune properties in macrophage cells. Methods: The cells were divided into two groups: As a control, the first was not treated with ACE, and the other was treated with ACE. Together with the cell viability, productions of nitric oxide (NO) and cytokines such as interleukin (IL)-$1{\beta}$, IL-6, and tumor necrosis factor (TNF)-${\alpha}$ by treating of ACE were monitored. Results: 1. There was no decrease of the cell viability after 24 hr incubation, but a significant decrease after 48 hr incubation with all four concentrations (25, 100, 200, and $400\;{\mu}g/m{\ell}$) of ACE. 2. A significant increase in the production of NO was observed in the concentrations above $50\;{\mu}g/m{\ell}$ of ACE after 24 hr incubation. 3. Further, after 48 hr incubation, the critical concentration of ACE for the increase was reduced to $25\;{\mu}g/m{\ell}$. 4. The production of (IL)-$1{\beta}$ significantly increased with the ACE concentrations of 100 and $200\;{\mu}g/m{\ell}$ after 24 hr incubation. 5. The production of IL-6 significantly increased with the ACE concentration of $200\;{\mu}g/m{\ell}$ after 24 hr incubation. 6. A significant increase in the production of (TNF)-${\alpha}$ was detected with ACE concentrations of 50, 100, and $200\;{\mu}g/m{\ell}$ after 24 hr incubation. Conclusions: These show that ACE increases mouse macrophage NO production at concentrations above $50\;{\mu}g/m{\ell}$, and the cytokines ((IL)-$1{\beta}$, IL-6, and (TNF)-${\alpha}$) at concentrations above $200\;{\mu}g/m{\ell}$. These results suggest that ACE improves macrophage immune property.

Keywords

References

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