Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Inhibitory Effect of Polysaccharide from Kefir Grain on the Infection of MA-104 Cell by Human Rotavirus

Kefir Grain의 Polysaccharide에 의한 HRV S-2의 MA-104 세포 감염억제

  • Lee, Jong-Ik (Department of Food Science and Biotechnology of Animal Resources, College of Animal Bioscience & Technology, Konkuk University) ;
  • Song, Kwang-Young (Department of Public Health, College of Veterinary Medicine, Konkuk University) ;
  • Chon, Jung-Whan (Department of Public Health, College of Veterinary Medicine, Konkuk University) ;
  • Hyeon, Ji-Yeon (Department of Public Health, College of Veterinary Medicine, Konkuk University) ;
  • Seo, Kun-Ho (Department of Public Health, College of Veterinary Medicine, Konkuk University)
  • 이종익 (건국대학교 동물생명과학대학 축산식품생물공학) ;
  • 송광영 (건국대학교 수의과대학 공중보건학) ;
  • 천정환 (건국대학교 수의과대학 공중보건학) ;
  • 현지연 (건국대학교 수의과대학 공중보건학) ;
  • 서건호 (건국대학교 수의과대학 공중보건학)
  • Received : 2010.08.25
  • Accepted : 2011.01.03
  • Published : 2011.02.28
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Abstract

The inhibitory effect of a polysaccharide from kefir on human rotavirus infection in MA-104 cells was investigated. The extracted polysaccharide was separated as fraction I in unbound materials and as fractions II, III, and IV in bound materials. Adding polysaccharide fractions II (4.8 mg/mL), III (5.3 mg/mL), and IV (1.4 mg/mL) inhibited the infection of MA-104 cells by human rotavirus. The 50% inhibitory concentrations (IC 50) were 0.075, 0.083, and 0.022 mg/mL, respectively. Based on these results, the kefir polysaccharide has anti-rotavirus activity. In conclusion, a polysaccharide from kefir had more than a 97% inhibition effect against human rotavirus infection in MA-104 cells.

본 연구에서는kefir중의 polysaccharide분획이 HRV의 MA-104세포 감염에 미치는 억제효과를 조사하였다. (1) 추출된 polysaccharide는 DEAE 이온교환 chromatography에서 fraction I의 미결합 분획과 fraction II, III, IV의 결합 분획으로 나뉘어졌다. (2) Polysaccharide fraction II, III, IV는 각각 4.8, 5.3, 1.4 mg/mL의 농도로 첨가했을 때 HRV의 MA-104 세포감염에 대하여 약 97%, 99%, 100%의 억제효과를 나타냈다. 본 연구의 결과를 종합하면 kefir의 다당체가 유아설사변에서 분리한 rotavirus S-2가 MA-104 cell 감염에 97% 이상 억제효과가 있어 동절기 유아의 바이러스성 급성설사 예방에 kefir를 급식하는 것이 바람직하다고 사료된다.

Keywords

References

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