Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Genotyping, Phage Typing, and Antimicrobial Resistance of Salmonella Typhimurium Isolated from Pigs, Cattle, and Humans

  • Ju, Min-Seok (National Veterinary Research and Quarantine Service, Incheon Regional Office, Incheon International Airport) ;
  • Kang, Zheng-Wu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Jung, Ji-Hun (Seoul Metropolitan Government Research Institute of Public Health and Environment) ;
  • Cho, Seong-Beom (National Food Safety and Toxicology Center, Michigan State University) ;
  • Kim, Sung-Hun (Division of Enteric Bacterial Infection, Center for Infectious Disease, National Institute of Health) ;
  • Lee, Young-Ju (College of Veterinary Medicine, Kyuongpook National University) ;
  • Hong, Chong-Hae (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Pak, Son-Il (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • Received : 2010.09.28
  • Accepted : 2011.01.07
  • Published : 2011.02.28
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Abstract

Salmonella enterica serovar Typhimurium (ST) is one of the most common serovars isolated from humans and animals. It has been suggested that ST infections in Koreans are largely due to the consumption of contaminated pork and beef. To investigate the genotypes, phage types, and antimicrobial resistance patterns for ST isolates of different origins, a total of 70 ST strains, including 19 isolates from humans, 44 isolates from pigs, and 6 isolates from cattle, were analyzed using pulsedfield gel electrophoresis (PFGE), phage typing, and antimicrobial susceptibility tests. Forty-three distinct PFGE patterns were generated from 70 ST isolates, which were grouped into 14 PFGE groups (from A to N) at the level of 75% similarity. The most prevalent group was the A (A1-A17 subtypes) group, encompassing 54.5% (38/70) of ST isolates. ST isolates from pigs and cattle mostly belong to groups A and L, whereas ST isolates from humans mostly belong to groups F and C. Antimicrobial susceptibility tests using 11 antimicrobial agents showed that resistance to tetracycline (TE) (81.4%) was highly prevalent, followed by streptomycin (S) (64.3%) and nalidixic acid (NA) (31.4%) resistance. A total of seventeen antimicrobial resistance patterns were observed. Only 8.6% of isolates, including a reference strain, were susceptible to all antimicrobial agents tested. The most prevalent resistance pattern was TE-S (37.1%), which was seen in 66.6% of bovine, 40.8% of swine and 21.1% of human isolates. Three ST isolates from humans (15.9%) showed resistance to 7-8 antimicrobials. The most predominant phage type (PT) was U302 (64.3%), followed by DT170 (10.0%). PFGE types did not coincide with antimicrobial resistance patterns and phage types; therefore, the combination of those types allowed for further differentiation between tested ST isolates.

Keywords

References

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