Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Antioxidant and Anticancer Effects of MeOH Extract of Liriodendron tulipifera

튤립(Liriodendron tulipifera) 나무가지 메탄올 추출물의 항산화와 항암활성 효과

  • Xu, Ming-Lu (Henan Institute of Science and Technology) ;
  • Wang, Lan (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University) ;
  • Wang, Myeong-Hyeon (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
  • 허명록 (하남과학기술학원) ;
  • 왕란 (강원대학교 의생명과학대학) ;
  • 왕명현 (강원대학교 의생명과학대학)
  • Received : 2010.12.17
  • Accepted : 2011.02.10
  • Published : 2011.02.28
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Abstract

In order to screen the functional constituents from nature resource, we studied the bioactivities of methanol extract of the Liriodendron tulipifera branch(MLT). The total phenolic and flavonoid contents, DPPH radical scavenging capacity, reducing power, $Fe^{2+}$ chelating ability, inhibition of lipid peroxidation and cell toxicity of MLT were investigated in this study. We found that the total phenolic and flavonoid content of MLT is 75.34 mg gallic acid/g and 20.15 mg quercetin/g respectively. MLT exhibited the antioxidant activity on DPPH radical with a $EC_{50}$ value of $289.68\;{\mu}g$/mL, the absorbance is 0.388 at $100\;{\mu}g$/mL in reducing power assay, MLT prevented 38.56% lipid peroxidation at $200\;{\mu}g$/mL. Furthermore, MLT exhibits the potent anti-proliferative activity which inhibited 56.94%, 35.73% growth of HT-29 and Hela cell at $200\;{\mu}g$/mL respectively. It showed that the antioxidant activities of MLT were correlated with its total phenolic and flavonoid contents. However further study need to be exploring in the future.

본 연구는 새로운 기능성 소재를 탐색하기 위하여 튤립나무 가지의 메탄올 추출물을 조제하여 생리활성 물질 함량, DPPH, 환원능력, $Fe^{2+}$ chelating효과와 지질과산화 억제 활성 그리고 세포독성을 측정하였다. 총 페놀성 화합물과 플라보노이드 함량은 75.34 mg gallic acid/g과 20.15 mg quercetin/g이고; DPPH 라디칼 소거 활성에서 $EC_{50}$은 $289.68{\pm}2.04 \;{\mu}g$/mL; 환원력 측정에서 $100\;{\mu}g$/mL 농도에서 흡광도는 0.388이었으며, $Fe^{2+}$ chelating 효과에서는 $200\;{\mu}g$/mL 농도에서 36.33%로 나타내었고, 지질과산화 억제 효능에서는 $200\;{\mu}g$/mL의 농도에서 비교적 높은 38.56%의 억제율을 나타내었으며, 암세포 증식 억제 효과에서는 HT-29와 Hela cell line에서 튤립나무가지 메탄올 추출물이 $200\;{\mu}g$/mL의 농도에서 56.94%와 35.73%의 세포생장 억제율을 나타내었다. 따라서 본 연구 결과들을 종합해 볼 때 항산화능력은 총 페놀성분과 밀접한 관계가 있는 것으로 사료되며, 튤립나무 가지에 대한 기타 생리활성 연구가 더 필요할 것으로 사료된다.

Keywords

References

  1. Banerjee, A., N. Dasgupta and B. De. 2005. In vitro study of antioxidant activity of Syzygium cumini fruit. Food Chem. 90:727-733. https://doi.org/10.1016/j.foodchem.2004.04.033
  2. Birt, B.F., S. Hendrich and W.Q. Wang. 2001. Dietary agents in cancer prevention: flavonoids and isoflavoids. Pharmacol. Ther. 90:157-177. https://doi.org/10.1016/S0163-7258(01)00137-1
  3. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200. https://doi.org/10.1038/1811199a0
  4. Buchanan, M.A. and E.E. Dickey. 1960. Liriodenine, a nitrogen-containing pigment of yellow poplar heartwood (Liriodendron tulipifera L.). J. Org. Chem. 25:1389-1391 https://doi.org/10.1021/jo01078a024
  5. Chang, C.C., M.H. Yang, H.M. Wen and J.C. Chern. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. of Food and Drug Analysis 10:178-182.
  6. Chen, C.L., H.M. Chang and E.B. Cowling. 1976. Aporphine alkaloids and lignans in heartwood of Liriodendron tulipifera. Phytochemistry 15:547-550. https://doi.org/10.1016/S0031-9422(00)88970-0
  7. Chen, C.L., H.M. Chang, E.B. Cowling, C.Y. Huang Hsu, R.P. Gates. 1976. Aporphine alkaloids and lignans formed in response to injury of sapwood in Liriodendron tulipifera. Phytochemistry 15:1161-1167. https://doi.org/10.1016/0031-9422(76)85122-9
  8. Chen, C.L. and H.M. Chang. 1978. Lignans and aporphine alkaloids in bark of Liriodendron tulipifera. Phytochemistry 17:779-782. https://doi.org/10.1016/S0031-9422(00)94226-2
  9. Choi, C.H., E.S. Song, J.S. Kim and M.H. Kang. 2003. Antioxidative activities of Castanea crenata Flos. methanol extracts. Kor. J. Food Sci. Technol. 35:1216-1220 (in Korean).
  10. Choi, S.Y., S.H. Lin, T.Y. Ha, S.R. Kim, K.S. Kang and I.K. Hwang. 2005. Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plant. Kor. J. Food Sci. Technol. 37:549-556.
  11. Cohen, J., W. Von Langenthal and W. Taylor. 1961. Notesthe alkaloids of Liriodendron tulipifera L. The structure and synthesis of the unnamed yellow alkaloid and the isolation of D-glaucine. J. Org. Chem. 26:4143-4144. https://doi.org/10.1021/jo01068a624
  12. Doskotch, R.W. and F.S. El-Feraly. 1969. Antitumor agents. II. Tulipinolide, a new ger-macranolide sesquiterpene, and constunolide. Two cytotoxic substances from Liriodendron tulipifera L. J. Pharm. Sci. 58:877-880. https://doi.org/10.1002/jps.2600580720
  13. Doskotch, R.W. and F.S. El-Feraly. 1970. The structure of tulipinolide and epitulipino- lide. Cytotoxic sesquiterpenes from Liriodendron tulipifera L. J. Org. Chem. 35:1928-1936. https://doi.org/10.1021/jo00831a046
  14. Doskotch, R.W., F.S. El-Feraly, E.H. Fairchild and C.-T. Huang. 1976. Peroxyferolide: a cytotoxic gerrnacranolide hydroperoxide from Liriodendron tulipifera. J. Chem. Soc. Chem. Comm. 402-403.
  15. Doskotch, R.W., F.S. El-Feraly, E.H. Fairchild and C.-T. Huang. 1977. Isolation and characterization of peroxyferolide, a hydroperoxy sesquiterpene lactone from Liriodendron tulipifera. J. Org. Chem. 42:3614-3618. https://doi.org/10.1021/jo00442a037
  16. Doskotch, R.W., S.L. Keely, C.D. Hufford, F.S. El-Feraly. 1975. New sesquiterpene lactones from Liriodendron tulipifera. Phytochemistry 14:769-773. https://doi.org/10.1016/0031-9422(75)83032-9
  17. Eom, S.H., H.J. Park, C.W. Jin, S.M. Park, M.J. Kim, C.Y. Yu and D.H. Cho. 2007. Changes of antioxidant activity in Juglans mandshurica Maxim. leaves by far infrared ray inradiation. Kor. J. Med. Crop Sci. 15:266-270 (in Korean).
  18. Goh, E.J., E.S. Seong, J.G. Lee, J.K. Na, J.D. Lim, M.J. Kim, N.Y. Kim, G.H. Lee, J.S. Seo, D.S. Cheoi, I.M. Chung and C.Y. Yu. 2009. Antioxidant activities according to peelling and cultivated years of Astragalus membranaceus Roots. Kor. J. Med. Crop Sci. 17:233-237 (in Korean).
  19. Gordon, M.F. 1990. The mechanism of antioxidant action in vitro. In Food antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p 1-18.
  20. Graziose, R., T. Rathinasabapathy, C. Lategan, A. Poulev, P.J. Smith, M. Grace, M.A. Lila and I. Raskin. 2010. Antiplasmodial activity of aporphine alkaloids and sesquiterpene lactones from Liriodendron tulipifera L. J. of Ethnopharmacology (in press).
  21. Green, L.M., J.L. Reade and C.F. Ware. 1984. Rapid colometric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J. of Immunological Methods 70:257-268. https://doi.org/10.1016/0022-1759(84)90190-X
  22. Hasegawa, G.R. 2007. Quinine substitutes in the confederate army. Military Medicine 172:650-655. https://doi.org/10.7205/MILMED.172.6.650
  23. Kang Y.H., Y.K. Park, S.R. Oh and K.D. Moon. 1995. Studies on the physiological functionality of ine needle and mugwort extracts. Kor. J. Food Sci. Technol. 27: 978-984 (in Korean).
  24. Kim, H.S. and Y.H. Kang. 2010. Antioxidant activity of ethanol extracts of non-edible parts (stalk, stem.leaf, seed) from oriental Melon. Kor. J. Plant Res. 23(5): 451-457 (in Korean).
  25. Kim, T.W. 1995. The woody plants of Korea in color, Kyo- Hak publishing Co. Ltd. Seoul. p. 101.
  26. Lee, J.H. and S.R. Lee. 1994. Analysis of phenolic substances content in Korean plants foods. Kor. J. Food Sci. Technol. 26:310-316 (in Korean).
  27. Lee, Y.R., M.Y. Kang, H.J. Koh, J.H. Chin and S.H. Nam. 2004. Screeing of physiological functionality of germainatedgiant embryonic rices. J. Kor. Soc. Appli. Biolo. Chem. 47:216-221.
  28. Lin, J.Y., and C.Y. Tang. 2007. Determination of total phenolic and flavonoid contents in selected freits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem. 101:140-147. https://doi.org/10.1016/j.foodchem.2006.01.014
  29. Merkle, S.A. and H.E. Sommer. 1991. Yellow-poplar (Liriodendron spp.). In YPS Bajaj, ed, Biotechnology in agriculture and forestry, trees III, Vol.16, Springer-verlag, Berlin, p. 94-110.
  30. Muhammad, I. and C.D. Hufford. 1989. Phenylpropanoids, sesquiterpenes, and alka- loids from the seeds of Liriodendron tulipifera. J. Nat. Prod. 52:1177-1179. https://doi.org/10.1021/np50065a048
  31. Rafinesque, C.S. and A. Atkinson. 1828. Medical Flora, or, Manual of the Medical Botany of the United States of North America. In: Rafinesque, C.S., Atkinson, Alexander (Eds.), Alexander, Philadelphia.
  32. Rhim, T.J. and M.Y. Choi. 2010. The antioxidative effects of Ampelopsis brevipedunculata extracts. Kor. J. Plant Res. 23(5):445-450 (in Korean).
  33. Spencer, C.F., F.R. Koniuszy, E.F. Rogers, J. Shavel, N.R. Easton, E.A. Kaczka, F.A. Kuehl, R.F. Phillips, A. Walti, K. Folkers, C. Malanga and A.O. Seeler. 1947. Survey of plants for antimalarial activity. Lloydia 10:145-174.
  34. Taylor, W.I. 1961. The structure and synthesis of liriodenine, a new type of isoquino- line alkaloid. Tetrahedron 14:42-45. https://doi.org/10.1016/0040-4020(61)80085-9
  35. Yen, G.C., P.D. Duhb, and H.L. Tsaia. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem. 79:307-313. https://doi.org/10.1016/S0308-8146(02)00145-0

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