Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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An Electrochemical Enzyme Immunochip Based on Capacitance Measurement for the Detection of IgG

  • Yi, Seung-Jae (Multiscale Multiphysics Measurement Laboratory, School of Mechanical Engineering, Pusan National University) ;
  • Choi, Ji-Hye (Institute of BioPhysio Sensor Technology, Pusan National University) ;
  • Kim, Hwa-Jung (Department of Microbiology and Research Institute for Medical Science, College of Medicine, Chungnam National University) ;
  • Chang, Seung-Cheol (Institute of BioPhysio Sensor Technology, Pusan National University) ;
  • Park, Deog-Su (Institute of BioPhysio Sensor Technology, Pusan National University) ;
  • Kim, Kyung-Chun (Multiscale Multiphysics Measurement Laboratory, School of Mechanical Engineering, Pusan National University) ;
  • Chang, Chulhun L. (Department of Laboratory Medicine, School of Medicine, and Medical Research Institute, Pusan National University)
  • Received : 2011.02.10
  • Accepted : 2011.02.21
  • Published : 2011.04.20
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Abstract

This study describes the development of an electrochemical array immunochip for the detection of IgG. Interdigitated immunochip platforms were fabricated by sputtering gold on a glass wafer by using MEMS process and then were coated with Eudragit S100, an enteric polymer, forming an insulating layer over the working area of immunochips. The breakdown of the polymer layer was exemplified by the catalytic action of urease which, in the presence of urea, caused an alkaline pH change. This subsequently caused an increase of the double layer capacitance of the underlying electrode. Used in conjunction with a competitive immunoassay format, this allowed the ratio of initial to final electrode capacitance to be directly linked with the concentration of analyte, i.e. IgG. Responses to IgG could be detected at IgG concentration as low as $250\;ngmL^{-1}$ and showed good linearity up to IgG concentration as high as $20\;{\mu}gmL^{-1}$.

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