Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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A Simple and Sensitive High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry Method for the Quantification of Ethyl Pyruvate in Rat Plasma

  • Kim, Hyun-Ji (Department of Molecular Science and Technology, Ajou University) ;
  • Kim, Seung-Woo (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University) ;
  • Lee, Ja-Kyeong (Department of Anatomy, Center for Advanced Medical Education, School of Medicine, Inha University) ;
  • Yoon, Sung-Hwa (Department of Molecular Science and Technology, Ajou University)
  • Received : 2010.09.10
  • Accepted : 2011.02.07
  • Published : 2011.04.20
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Abstract

Ethyl pyruvate (EP) is known as a scavenger of reactive oxygen species (ROS) in the body through its role in the donation of diketone groups to metals to form an EP-metal complex. In order to develop a method for the quantification of EP in biological media, a sensitive and specific, high-performance liquid chromatographyelectrospray ionization-mass spectrometry (HPLC-ESI/MS) method is used to determine the EP-alkali metal ion binding species. The analyte was separated on a ZORBOX SB-C8 ($3.5{\mu}m$, $30mm{\times}2.1mm$ I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the m/z 255 $[2M + Na]^+$ ion. The method was validated over the concentration range of $0.5-60.0\;{\mu}g$/mL under 1/9 (v/v) of acetonitrile/methanol solvent system with flow rate 0.05 mL/min. The limit of quantification (LOQ) was $0.5{\mu}g$/mL.

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References

  1. Varma, S. D.; Devamanoharan, P. S.; Ali, A. H. Free Radical Res.1998, 28, 131. https://doi.org/10.3109/10715769809065799
  2. Sims, C. A.; Wattanasirichaigoon, S.; Menconi, M. J.; Ajami, A.M.; Fink, M. P. Crit. Care Med. 2001, 29, 1513. https://doi.org/10.1097/00003246-200108000-00003
  3. Ulloa, L.; Ochani, M.; Yang, H.; Tanovic, M.; Halperin, D.; Yang,R.; Czura, C. J.; Fink, M. P.; Tracey, K. J. Proc. Natl. Acad. Sci. U. S. A. 2002, 99, 12351. https://doi.org/10.1073/pnas.192222999
  4. Bunger, R.; Mallet, R. T.; Hartman, D. A. Eur. J. Biochem. 1989,180, 221. https://doi.org/10.1111/j.1432-1033.1989.tb14637.x
  5. Cicalese, L.; Lee, K.; Schraut, W. Am. J. Surg. 1999, 171, 97.
  6. Unal, B.; Karabeyoglu, M.; Huner, T.; Canbay, E.; Eroglu, A.;Yildirim, O.; Dolapci, M.; Bilgihan, A.; Cengiz, O. Surgical Innovation 2009, 16, 21.
  7. Sappington, P. L.; Fink, M. E.; Yang, R.; Delude, R. L.; Fink, M.P. Shock 2003, 20, 521. https://doi.org/10.1097/01.shk.0000092697.10326.8b
  8. Tawadrous, Z. S.; Delude, R. L.; Fink, M. P. Shock 2002, 17, 473. https://doi.org/10.1097/00024382-200206000-00006
  9. Venkataraman, R.; Kellum, J. A.; Song, M.; Fink, M. P. Shock2002, 18, 507. https://doi.org/10.1097/00024382-200212000-00004
  10. Liu, J.; Segal, M.; Yoo, S.; Yang, G. Y.; Kelly, M.; James, T. L.;Litt, L. Neurochemistry International 2009, 54, 106. https://doi.org/10.1016/j.neuint.2008.10.009
  11. Dave, S. H.; Tilstra, J. S.; Matsuoka, K.; Li, F.; DeMarco, R. A.;Beer-Stolz, D.; Sepulveda, A. R.; Fink, M. P.; Lotze, M. T.; Plevy,S. E. Journal of Leukocyte Biology 2009, 86, 633. https://doi.org/10.1189/jlb.1008662
  12. Liang, X.; Chavez, A.; Schapiro, N. E.; Loughran, P.; Thorne, S.H.; Amoscato, A. A.; Zeh, H. J.; Beer-Stolz, D.; Lotze, M. T.;Vera, M. E. Journal of Leukocyte Biology 2009, 86, 599 https://doi.org/10.1189/jlb.0908578
  13. Han, Y.; Englert, J. A.; Yang, R.; Delude, R. L.; Fink, M. P. TheJournal of Pharmacology and Experimental Therapeutics 2005,312, 1097.
  14. Lee, J. Y.; Lee, J. Y.; Lee, S. E. Invest Ophthalmol. Vis. Sci. 2004,45, 1523. https://doi.org/10.1167/iovs.03-1315
  15. Rsung, A.; Kaizu, T.; Nakao, A. Transplantation 2005, 27, 196.
  16. Varma, S. D.; Devamanoharan, P. S.; Ali, A. H. Free Rad. Res.2000, 33, 23. https://doi.org/10.1080/10715760000300581
  17. Cho, I. H.; Kim, S. Y.; Kim, J. B.; Kim, T. K.; Lee, K. W.; Han, P.L.; Lee, J. K. J. of Neuroscience Research 2006, 84, 1505. https://doi.org/10.1002/jnr.21052
  18. Yu, Y. M.; Kim, J. B.; Lee, K. W.; Kim, S. Y.; Han, P. L.; Lee, J. K.Stroke 2005, 36, 2238. https://doi.org/10.1161/01.STR.0000181779.83472.35
  19. Shimizu, C.; Nara, Y.; Takashio, M. J. Am. Soc. Brew. Chem.2005, 63, 135.
  20. Bartok, M.; Bartok, T.; Szollosi, G.; Felfoldi, K. J. Mass Spectrom.2002, 37, 1034. https://doi.org/10.1002/jms.359
  21. Bartok, M.; Kele, Z.; Kortvelyesi, T.; Bartok, T.; Felföldi, K.Rapid Commun. Mass Spectrom. 2003, 17, 2849. https://doi.org/10.1002/rcm.1253
  22. Bartok, M.; Szabo, P. T.; Bartok, T.; Szollosi, G. Rapid Commun Mass Spectrom. 2000, 14, 509. https://doi.org/10.1002/(SICI)1097-0231(20000331)14:6<509::AID-RCM902>3.0.CO;2-P
  23. Saraiva, M. A.; Borges, C. M.; Florêncio, M. H. J. Mass Spectrom.2006, 41, 216. https://doi.org/10.1002/jms.980
  24. Kebarle, P. J. Mass Spectrom. 1997, 32, 922. https://doi.org/10.1002/(SICI)1096-9888(199709)32:9<922::AID-JMS577>3.0.CO;2-C
  25. Loo, J. A. Int. J. Mass Spectrom. 2000, 200, 175. https://doi.org/10.1016/S1387-3806(00)00298-0
  26. Emmert, J.; Pfluger, M.; Wahl, F. LC.GC Europe 2004, 17, 646.
  27. Zhang, D.; Du, X.; Liu, M.; Li, H.; Jing, Y.; Zhao, L.; Gu, J. J. Chromatogr. B 2008, 863, 223. https://doi.org/10.1016/j.jchromb.2008.01.013
  28. Snyder, L. R.; Kirkland, J. J.; Glach, J. L. Practical HPLC Method Development; John Wiley & Sons Inc.: New York, 1997; p 659.

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