The Journal of Korean Physical Therapy

  • 제23권5호
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  • Pages.35-41
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  • 2011
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  • 1229-0475(pISSN)
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  • 2287-156X(eISSN)
대한물리치료학회 (The Korean Society of Physical Therapy)
권호 표지

유전자 조작된 PRV-BaBlu를 이용한 흰쥐 위 신경지배 편도핵의 동정

Localization of Amygdaloid Nucleus Innervating the Stomach Using Genetically Engineered PRV-BaBlu in Rat Brain

  • 송주민 (대구대학교 재활과학대학 물리치료학과 신경과학교실)
  • Song, Ju-Min (Laboratory of Neuroscience, Department of Physical Therapy, College of Rehabilitation Science, Daegu University)
  • 투고 : 2011.06.30
  • 심사 : 2011.09.26
  • 발행 : 2011.10.25
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초록

Purpose: This study was carried out to investigate the spatiotemporal localization of the amygdaloid nucleus innervating the rat stomach using PRV-BaBlu, which has been known to be an excellent type of neurotracer with the ability to transpass the neuronalsynaptic cleft. Methods: Ninety Sprague-Dawley rats (250~300 g) that were injected with PRV-BaBlu into the stomach were randomly divided into 3, 4 and 5 day groups (each group n=30). $2{\mu}l$ of PRV-BaBlu, a genetically modified strain of PRV-Bartha with the lac-Z gene,was injected into the rat stomach and immunostained with a mouse anti-${\beta}$-galactosidase at 3, 4 and 5 days after the virus injection. Results: The PRV-BaBlu infected the neurons in the amygdaloid nucleus, and the degree of viral infection in experimental animals showed a tendency to increase significantly with time (p<0.05). The neurons between the left and right amygdaloid nucleus significantly differ (p<0.05). Conclusion: This showed that PRV-BaBlu was an excellent neurotracer for localizing the amygdaloid nucleus, and the amygdaloid nucleus has a sensory input and motor output on stomach movement, influencing emotional behavior.

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참고문헌

  1. Lamprecht R, Margulies DS, Farb CR, et al. Myosin light chain kinase regulates synaptic plasticity and fear learning in the lateral amygdala. Neuroscience. 2006;139(3):821-9. https://doi.org/10.1016/j.neuroscience.2005.12.055
  2. Cheng DT, Knight DC, Smith CN et al. Functional MRIof human amygdala activity during pavlovian fear conditioning: Stimulus processing versus response expression. Behav Neurosci. 2003;117(1):3-10. https://doi.org/10.1037/0735-7044.117.1.3
  3. Perez de la MM, Jacobsen KH, Crespo-Ramırez M, et al. Wiring and volume transmission in rat amygdala. Implications for fear and anxiety. Neurochem. Res. 2008; 33(8):1618-33. https://doi.org/10.1007/s11064-008-9722-9
  4. Rosen JB, Donley MP. Animal studies of amygdala function in fear and uncertainty: Relevance to human research. Biol Psychol. 2006;73(1):49-60. https://doi.org/10.1016/j.biopsycho.2006.01.007
  5. Rinaman L, Roesch MR, Card JP. Retrograde transynaptic pseudorabies virus infection of central autonomic circuits in neonatal rats. Brain Res Dev Brain Res. 1999;114(2): 207-16. https://doi.org/10.1016/S0165-3806(99)00039-5
  6. La Fleur SE, Klasbeek A, Wortel J et al. Polysynaptic neural pathways between the hypothalamus, including the suprachiasmatic nucleus, and the liver. Brain Res. 2000;871(1): 50-6. https://doi.org/10.1016/S0006-8993(00)02423-9
  7. Ugolini G. Rabies Virus as a Transneuronal Tracer of Neuronal Connections. Adv Virus Res. 201179:165-202.
  8. Gerendai I, Toth LE, Kocsis K, et al. Transneuronal labelling of nerve cells in the cns of female rat from the mammary gland by viral tracing technique. Neuroscience. 2001;108(1): 103-18. https://doi.org/10.1016/S0306-4522(01)00399-2
  9. Schramm LP, strack AM, Platt KB et al. Peripheral and central pathways regulating the kidney: A study using pseudorabies virus. Brain Res. 1993;616(1-2):251-62. https://doi.org/10.1016/0006-8993(93)90216-A
  10. Kalsbeek A, Fliers E, Franke AN et al. Functional connections between the suprachiasmatic nucleus and the thyroid gland as revealed by lesioning and viral tracing techniques in the rat. Endocrinology. 2000;141(10):3832-41. https://doi.org/10.1210/en.141.10.3832
  11. Vizzard MA, Brisson M, de Groat WC. Transneuronal labeling of neurons in theadult rat central nervous system following inoculation of pseudorabies virus into the colon. Cell Tissue Res. 2000;299(1):9-26. https://doi.org/10.1007/s004410050002
  12. Niedringhaus M, Jackson PG, Pearson R. Brainstem sites controlling the lower esophageal sphincter and crural diaphragm in the ferret: A neuroanatomical. Auton Neurosci. 2008;144(1-2);50-60. https://doi.org/10.1016/j.autneu.2008.09.007
  13. Vizzard MA, Erickson VL, Card JP et al. Transneuronal labeling of neurons in the adult rat brainstem and spinal cord after injection of pseudorabies virus into the urethra. J Comp Neurol. 1995;355(4):629-40. https://doi.org/10.1002/cne.903550411
  14. Sly DJ, Colvill,Mckinley MJ, Oldfield BJ. et al. Identification of neural projections from the forebrain to the kidney, using the virus pseudorabies. J Auton Nerv Syst. 1999;77(2-3): 73-82. https://doi.org/10.1016/S0165-1838(99)00031-4
  15. Gerendai I, Toth LE, Kocsis K, et al. Identification of cns neurons involved in the innervation of the epididymis: A viral transneuronal tracing study. Auton Neurosci. 2001; 92(1-2):1-10. https://doi.org/10.1016/S1566-0702(01)00292-2
  16. Dobbins EG, Feldman JL. Differential innervation of protruder and retractor muscles of the tongue in rat. J Comp Neurol. 1995;357(3):376-94. https://doi.org/10.1002/cne.903570305
  17. FontAin JJ, Diec CT, Velloff CR. Bilateral distribution of vagal motor and sensory nerve fibers in the rat's lungs and airways. Am J Physiol Regul Integr Comp Physiol. 2000; 279(2):R713-28. https://doi.org/10.1152/ajpregu.2000.279.2.R713
  18. Dobbins EG, Feldman JL. Brainstem network controlling descending drive to phrenic motoneurons in rat. J Comp Neurol. 1994;347(1):64-86. https://doi.org/10.1002/cne.903470106
  19. Gerendai I, Kocsis K, Halasz B. Supraspinal connections of the ovary: Structural and functional aspects. Microsc Res Tech. 2002;59(6):474-83. https://doi.org/10.1002/jemt.10225
  20. Loewy AD, Haxhiu MA. Cns cell groups projecting to pancreatic parasympathetic preganglionic neurons. Brain Res. 1993;620(2):323-30. https://doi.org/10.1016/0006-8993(93)90174-L
  21. Lee JH, Park CE, Park RJ. Electrical Stimulation Induces the Collagen DepositionTGF-${\beta}$1 mRNA Expression in Skin Wound of Rat. Kor Soc Phys Ther. 22(3):87-92. https://doi.org/10.1589/jpts.22.87
  22. Xu C, Giuliano F, Yaici ED, et al. Identification of lumbar spinal neurons controlling simultaneously the prostate and the bulbospongiosus muscles in the rat. Neuroscience. 2006;138(2):561-73. https://doi.org/10.1016/j.neuroscience.2005.11.016
  23. Lee HM. Chae YW. Influence of Microcurrent Therapy in Interleukin.1 Expression in Rhueumatoid Arthritis Rats. J Kor Soc Phys Ther. 2009;21(2):103-8.
  24. Cho MS. The Effect of Electroacupuncture on NeuN Expression in Spinal Cord in Sciatic Nerve Injured Rat. J Kor Soc Phys Ther. 2011;23(2):45-52. https://doi.org/10.1589/jpts.23.45
  25. Oldfield BJ, Giles ME, Watson A et al. The neurochemical characterisation of hypothalamic pathways projecting polysynaptically to brown adipose tissue in the rat. Neuroscience. 2002;110(3):515-26. https://doi.org/10.1016/S0306-4522(01)00555-3
  26. Larsen PJ, Enquist LW, Card JP. Characterization of the multisynaptic neuronal control of the rat pineal gland using viral transneuronal tracing. Eur J Neurosci. 1998;10(1): 128-45. https://doi.org/10.1046/j.1460-9568.1998.00003.x
  27. Daele V, Agassandian FK, Cassell MD. Amygdala connections with jaw, tongue and laryngo-pharyngeal premotor neurons. Neuroscience. 2011;177:93-113.
  28. Ugolini G. Advances in viral transneuronal tracing. J Neurosci Methods. 2010;194(1):2-20 https://doi.org/10.1016/j.jneumeth.2009.12.001
  29. Callaway EM. Transneuronal circuit tracing with neurotropic viruses. Curr Opin in Neurobiol. 2008;18(6):617-23. https://doi.org/10.1016/j.conb.2009.03.007
  30. Rotto Percelay DM, Wheeler JG, Osorio FA et al. Transneuronal labeling of spinal interneurons and sympathetic preganglionic neurons after pseudorabies virus injections in the rat medial gastrocnemius muscle. Brain Res. 1992; 574(1-2):291-306. https://doi.org/10.1016/0006-8993(92)90829-X
  31. Denes A, Boldogkoi Z, Hornyak A. Attenuated pseudorabies virus-evoked rapid innate immune response in the rat brain. J Neuroimmunol. 2006;180(1-2):88-103. https://doi.org/10.1016/j.jneuroim.2006.07.008
  32. Orr R, Marson L. Identification of cns neurons innervating the rat prostate: A transneuronal tracing study using pseudorabies virus. J Auton Nerv Syst. 1998;72(1):4-15. https://doi.org/10.1016/S0165-1838(98)00079-4
  33. Malkani S, Wallace KJ, Donley MP et al. An egr-1 (zif268) antisense oligodeoxynucleotide infused into the amygdala disrupts fear conditioning. Learn Mem. 2004; 11(5):617-24. https://doi.org/10.1101/lm.73104