Animal cells and systems

  • 제15권2호
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  • Pages.123-130
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  • 2011
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
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Transient receptor potential melastatin type 7 channels are involved in zinc-induced apoptosis in gastric cancer

  • Kim, Byung-Joo (Division of Longevity and Biofunctional Medicine, Pusan National University, School of Korean Medicine)
  • 투고 : 2010.09.24
  • 심사 : 2011.03.10
  • 발행 : 2011.06.30
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초록

Transient receptor potential melastatin 7 (TRPM7) channels are novel $Ca^{2+}$-permeable non-selective cation channels that are ubiquitously expressed. Activation of TRPM7 channels has been shown to be involved in the survival of gastric cancer cells. Here we show evidence suggesting that TRPM7 channels play an important role in $Zn^{2+}$- mediated cellular injury. Using a combination of electrophysiology, pharmacological analysis, small interfering RNA (siRNA) methods and cell death assays, we showed that activation of TRPM7 channels augmented $Zn^{2+}$-induced apoptosis of AGS cells, the most common human gastric adenocarcinoma cell line. The $Zn^{2+}$-mediated cytotoxicity was inhibited by the non-specific TRPM7 blockers $Gd^{3+}$ or 2 aminoethoxydiphenyl borate (2-APB) and TRPM7 specific siRNA. In addition, we showed that overexpression of TRPM7 channels in HEK293 cells increased $Zn^{2+}$- induced cell injury. Thus, TRPM7 channels may represent a novel target for physiological disorders where $Zn^{2+}$ toxicity plays an important role.

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피인용 문헌

  1. Role of transient receptor potential melastatin type 7 channel in gastric cancer vol.5, pp.2, 2011, https://doi.org/10.1016/j.imr.2016.04.004