Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Moderating Effects of Skin Hyperpigmentation from Lycii fructus and Lycii folium Extracts

구기자 및 구기엽 추출물의 피부과색소 조절효과

  • Received : 2011.06.30
  • Accepted : 2011.11.04
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of the present study was to evaluate the skin whitening effect of the extracts, Lycii fructus (LF), Dry-L. foilum (DLF) and Fresh-L. folium (FLF). Tyrosinase inhibition activities was 44% in DLF ethanol extracts at a $500{\mu}g/mL$. When the tyrosinase activities in B16F10 murine melanoma cell were tested, the activities in DLF ethanol extracts was 14% at a $50{\mu}g/mL$ concentration. The protein expression of microphthalmia-associated transcription factor, tyrosinase related protein 1 (TRP-1), TRP-2, and tyrosinase, which are all melanin related factors, showed that LF, DLF and FLF extracts inhibited the protein bio-synthesis in B16F10 melanoma cell. Especially the DLF extract showed greater decrease of protein expressions. Results indicate that the DLF extract tested in the present study had skin whitening activity and can be used as a function a ingredients for food and cosmetic compositions.

구기자, 건조구기엽, 생구기엽 추출물의 미백효과를 검증하였다. Tyrosinase 저해활성 측정결과 건조구기엽 에탄올추출물 $500{\mu}g/mL$에서 44%의 저해활성을 나타내었으며, melanoma cell (B16F10)에서의 melanin 생합성 저해율 측정결과 건조구기 엽 에탄올 추출물 $50{\mu}g/mL$에서 14%의 저해효과를 나타내었다. 구기자, 건조구기엽, 생구기엽 추출물을 처리한 B16F10군에서는 tyrosinase protein의 발현이 처리하지 않은 군보다 감소하였다. 특히, 건조구기엽 추출물의 경우 tyrosinase의 단백질 발현량을 많이 저해하였다. 결과적으로 건조구기엽 추출물의 미백활성이 가장 우수하였으며, 식품 및 화장품의 기능성 소재로 이용이 가능할 것으로 판단된다.

Keywords

References

  1. Ahn TH and Cho JW (2011) Investigation of antioxidant activity on Chinese matrimony vine (Lycium Chinense M.) Germplasm. MS Thesis, Chungnam National University, Daejon, Korea.
  2. Akiu S, Suzuki Y, Asahara T, Fujinuma Y, and Fukuda M (1991) Inhibitory effect of arbutin on melanogenesis-biochemical study using cultured B16 melanoma cells. Nippon Hifuka Gakkai Zasshi 101, 609-613.
  3. Alvaro SF, Jose Neptuno RL, and Francisco GC (1995) Tyrosinase a comprehensive review of its mechanism. Biochim Biophys Acta 1247, 1-11. https://doi.org/10.1016/0167-4838(94)00204-T
  4. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, and Mitchell JB (1987) Evaluation of a tetrazolium based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47, 936-942.
  5. Chakraborty AK (1998) Effect of arbutin on melanogenic proteins in human melanocytes. Pigment Cell Res 11, 206-212. https://doi.org/10.1111/j.1600-0749.1998.tb00731.x
  6. Choe YS (2006) The effect of basic cosmetic ingredients on the melanogenesis in the B16F10 mouse melanocyte. Konkuk University, Seoul, Korea.
  7. Choi BW, Lee BH, Kang KJ, Lee ES, and Lee NH (1998) Screening of the tyrosinase inhibitors from marine algae and medicinal plants. Korean J Pharmacogn 29, 237-242.
  8. Curto EV (1992) Inhibitors of mammalian melanocutes tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem Pharmacol 57, 663-672.
  9. Ha TK (2009) Inhibitory effect of Fritillaria verticillata willd. var. thunbergii bak ethanol extract on melanin biosynthesis. MD Thesis, Sangji University, Wonju, Korea.
  10. Han J, Kim HJ, Lee SS, and Lee IS (1999) Inhibitive effects of meju extracts made with a single inoculum of the fungi isolated from the traditional meju on the human leukemia cell line. Mycobiology 27, 312-317.
  11. Hearing VJ (1999) Biochemical control of melanogenesis and melanosomal organization. J Invest Dermatol 4, 24-28. https://doi.org/10.1038/sj.jidsp.5640176
  12. Hong SJ (2008) Scutellaria baicalensis georgi (SBG) inhibits melanin synthesis in mouse B16 melanoma cells. Ph D Thesis, Kyungwon University, GyeongGi, Korea.
  13. Hosoi J, Abe E, Suda T, and Kuroki T (1985) Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 45, 1474-1478.
  14. Kang JR, Lee MK, and Kang SM (2008) Antioxidant property and tyrosinase inhibition activity of various extracts from plants in compositae plants. J Korean Soc Appl Biol Chem 51, 321-328. https://doi.org/10.3839/jksabc.2008.056
  15. Kim SS, Ha JH, Jeong MH, Ahn JH, Yoon WB, Park SJ, Seong DH, and Lee HY (2009) Comparison of biological activities of fermented Codonopsis lanceolata and fresh Codonopsis lanceolata. Korean J Medicinal Crop Sci 17, 280-285.
  16. Kim TS, Park WJ, Ko SB, and Kang MH (2008) Development of extracts of Lycii folium having high anti-oxidant activity. J Korean Soc Food Sci Nutr 37, 1318-1322. https://doi.org/10.3746/jkfn.2008.37.10.1318
  17. Kwak JH, Seo UK, and Han YH (2001) Inhibitory effect of mugwork extracts on tyrosinase activity. Korean J Biotechnol Bioeng 16, 220-223.
  18. Lee HC, Lee BC, Kim SD, Lee KS, Paik SW, Lee SS, and Kim SM (2008) Change in composition of Gugija (Lycii fructus) species according to harvest time. Korean J Medicinal Crop Sci 16, 306-312.
  19. Mishima Y, Hatta S, Ohyama Y, and Inazu M (1988) Induction of melanogenesis suppression: Cellular pharmacology and mode of differential action. Pigment Cell Res 1, 367-374. https://doi.org/10.1111/j.1600-0749.1988.tb00136.x
  20. Na SH (2009) Paeonia suffruticosa Andr (PSA) inhibits melanin synthesis in mouse B16 melanoma cells. PH. D. Thesis, Kyung Hee University, Yongin, Korea.
  21. Park BH, Cho HS, and Kim DH (2005) Anti-oxidative effects of solvent extracts of Lycii fructus powder (LFP) and Maejakgwa made with LFP. J Korean Soc Food Sci Nutr 34, 1314-1319. https://doi.org/10.3746/jkfn.2005.34.9.1314
  22. Park SJ, Park WJ, Lee BC, Kim SD, and Kang MH (2006) Antioxidative activity of different species Lycium Chinensis Miller extracts by harvest time. J Korean Soc Food Sci Nutr 35, 1146-1150. https://doi.org/10.3746/jkfn.2006.35.9.1146
  23. Park YJ, Kim MH, and Bae SJ (2002) Enhancement of anticarcinogenic effect by combination of Lycii fructus with vitamin C. J Korean Soc Food Sci Nutr 31, 143-148. https://doi.org/10.3746/jkfn.2002.31.1.143
  24. Veronique DM and Friedrich B (1999) Tyrosinase and related proteins in mammalian pigmentation. FEBS Lett 381, 165-168.
  25. Yagi A, Kanbara T, and Morinobu N (1986) The effect of tyrosinase inhibition for aloe. Planta Medica 3981, 517-519.
  26. Yamamura T, Onishi J, and Nishiyama T (2002) Antimelanogenic activity of hydrocoumarins in cultured normal human melanocytes by stimulating intracellular glutathione synthesis. Arch Dermatol Res 294, 349-354.

Cited by

  1. Effects of Ehwa Makgeolli Containing Oriental Herbs on Skin Whitening and Wrinkles vol.42, pp.4, 2013, https://doi.org/10.3746/jkfn.2013.42.4.550
  2. Effects of Lycii Fructus Extracts(LFE) on Skin whitening and Elasticity using Melanoma cells vol.27, pp.1, 2014, https://doi.org/10.6114/jkood.2014.27.1.058
  3. 지골피와 생강 정제 추출물이 고지방식이 흰쥐의 체내 지질과 혈청 Cytokine 수준에 미치는 영향 vol.45, pp.5, 2011, https://doi.org/10.4163/kjn.2012.45.5.411
  4. 당귀와 구기자 에탄올 추출물의 피부 미백작용 vol.21, pp.1, 2013, https://doi.org/10.14374/hfs.2013.21.1.091
  5. 친환경 구기자재배지에서 해충의 계절 발생소장 및 친환경유기농자재의 방제효과 vol.17, pp.4, 2011, https://doi.org/10.7585/kjps.2013.17.4.402
  6. 친환경 구기자재배지에서 다년간 해충발생양상 vol.41, pp.4, 2011, https://doi.org/10.7744/cnujas.2014.41.4.341
  7. 구기자에서 큰이십팔점박이무당벌레(Henosepilachna vigintioctomaculata) 의 온도조건에 따른 발육특성과 친환경제재에 의한 방제효과 vol.41, pp.4, 2014, https://doi.org/10.7744/cnujas.2014.41.4.361
  8. 가공용 소재로서 구기자나무 잎의 건조온도 조건 vol.23, pp.6, 2011, https://doi.org/10.7783/kjmcs.2015.23.6.468
  9. 구기자 종자 초임계유체 추출물의 화장품소재로서의 가능성 평가 vol.34, pp.3, 2017, https://doi.org/10.12925/jkocs.2017.34.3.623
  10. 구기자나무의 재식밀도 및 예취높이가 가공용 잎 생산에 미치는 영향 vol.28, pp.2, 2020, https://doi.org/10.7783/kjmcs.2020.28.2.136
  11. 구기자 잎 생산에 알맞은 품종, 예취시기 및 생리활성 평가 vol.33, pp.5, 2011, https://doi.org/10.7732/kjpr.2020.33.5.436