Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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In vitro Antioxidant Potential and Oxidative DNA Damage Protecting Activity of the Ethanol Extracts of Cacalia firma Komar

병풍쌈의 에탄올 추출물의 항산화 기능 및 DNA의 산화적 손상 억제작용

  • Lee, Jin-Ha (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Bong-Gyeong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Park, Ae-Ri (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Kye-Jhae (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Choi, Dae-Woon (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Han, Sang-Hwa (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Choi, Geun-Pyo (Gangwon Provincial College) ;
  • Kim, Jong-Dai (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Jin-Chul (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Ahn, Ju-Hee (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Shin, In-Chul (Department of Biochemistry, College of Natural Sciences, Kangwon National University) ;
  • Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University)
  • 이진하 (강원대학교 바이오산업공학부) ;
  • 이봉경 (강원대학교 바이오산업공학부) ;
  • 박애리 (강원대학교 바이오산업공학부) ;
  • 이계재 (강원대학교 바이오산업공학부) ;
  • 최대운 (강원대학교 바이오산업공학부) ;
  • 한상화 (강원대학교 자연과학대학 생화학과) ;
  • 최근표 (강원도립대학 식품가공제과제빵학과) ;
  • 김종대 (강원대학교 바이오산업공학부) ;
  • 김진철 (강원대학교 바이오산업공학부) ;
  • 안주희 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 신인철 (강원대학교 자연과학대학 생화학과) ;
  • 박희준 (상지대학교 보건과학대학, 제약공학과)
  • Received : 2011.03.03
  • Accepted : 2011.08.26
  • Published : 2011.12.31
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Abstract

The Korean edible mountainous vegetable, byeongpungssam, Cacalia firma Komar. (CFK) is a wild plant found in the intermountain areas in Korea. The aim of this study was to investigate its free radical scavenging activity using 1,1-diphenyl-2-picryl-hydrazyl, 2,2-azinobis(3-ethylbenozothiazoline- 6-sulfonic acid) diammonium salt, ferric reducing/antioxidant power assays, an electron spin resonance spectroscopy. We also examined its protective effect against oxidative DNA damage using agarose electrophoresis of ethanol extract of CFK. The protective activity of the extract against the DNA damage induced by HO${\cdot}$ radicals was compared to epicatechin, ascorbic acid and trolox as reference antioxidant compounds. Total phenolic content in the extract was determined spectrometrically according to the Folin-Ciocalteu procedure and calculated as gallic acid equivalents. Total polyphenolic content of the extract was measured in the leaves ($161.53{\pm}1.07{\mu}g/g$) and shoot ($142.45{\pm}0.56{\mu}g/g$). The antioxidant potential of the extracts against some radicals and DNA damage by HO${\cdot}$ radicals showed over 60%, respectively.

산나물의 일종인 병풍쌈[Cacalia firma Komar. (CFK)]은 산지에서는 인기 있는 자생식품 자원이다. 본 연구의 목적은 CFK의 ethanol (EtOH) 추출물의 1,1-diphenyl-2-picryl-hydrazyl, 2,2-azinobis(3-ethylbenozothiazoline-6-sulfonic acid) diammonium salt, ferric reducing/antioxidant power 분석법 및 electron spin resonance spectroscopy에 의한 유리기의 억제작용과 agarose electrophoresis법에 의한 산화적 DNA damage에 대한 보호효과를 조사하는 것이다. HO${\cdot}$ 유리기에 의한 CFK EtOH 추출물의 DNA damage 보호효과는 항산화 참고 물질로 epicatechin, ascorbic acid 및 trolox등과 비교하였다. CFK EtOH 추출물 중의 총 페놀물질 분석은 Folin-Ciocalteu의 방법으로 행하고 표준물질로 gallic acid equivalents를 사용하여 함량을 표시하였다. CFK EtOH 추출물 중의 총 페놀 함량은 잎 과 줄기 부분에서 각각 $161.53{\pm}1.07{\mu}g/g$, $142.45{\pm}0.56{\mu}g/g$이 확인 되었다. CFK EtOH 추출물 중의 유리기들에 대한 항산화 기능과 HO${\cdot}$기에 의한 DNA 손상에 대한 보호효과는 각각 약 60% 이상의 기능을 보였다.

Keywords

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