Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Cytotoxic Effect of Flavonoids from the Roots of Glycyrrhiza uralensis on Human Cancer Cell Lines

감초(Glycyrrhiza uralensis Fisch.)로부터 분리된 flavonoid의 인체 암세포에 대한 세포독성

  • Park, Ji-Hae (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Wu, Qian (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University) ;
  • Yoo, Ki-Hyun (Department of Genetic Engineering, Kyung Hee University) ;
  • Yong, Hye-Im (Research Division of Food Industry Promotion, Korea Food Research Institute) ;
  • Cho, Sueng-Mock (Research Division of Food Industry Promotion, Korea Food Research Institute) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 박지해 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 우치엔 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 유기현 (경희대학교 유전공학과) ;
  • 용혜임 (한국식품연구원 산업진흥연구본부) ;
  • 조승목 (한국식품연구원 산업진흥연구본부) ;
  • 정인식 (경희대학교 유전공학과) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Received : 2011.11.25
  • Accepted : 2011.03.03
  • Published : 2011.03.31
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Abstract

The roots of Glycyrrhiza uralensis Fisch. were extracted with 30% aqueous ethanol (EtOH), and the concentrated extract was partitioned with n-hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. From the $CHCl_3$ fraction, four flavonoids were isolated through the repeated silica gel ($SiO_2$), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographies (c.c.). According to the results of spectroscopic data including nuclear magnetic resonance spectrometry (NMR), electron ionization mass spectrometry (EI/MS), and infrared spectroscopy (IR), the chemical structures of the compounds were determined as glabrol (1), abyssinone II (2), glabridin (3), and isoliquiritigenin (4). The flavonoids were evaluated for cytotoxic effect against human cancer cell lines, HCT-116, HepG2, HeLa, SK-OV-3, SK-BR-3, MCF-7, and SK-MEL-5. Especially, glabrol (1) and glabridin (2) showed $IC_{50}$ values of lower than $25{\mu}M$.

Keywords

References

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