Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Effects of Transgenic Soybean Cultivation on Soil Microbial Community in the Rhizosphere

형질전환 콩 재배가 근권 토양 미생물상에 미치는 영향

  • 이기종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 손수인 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이장용 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이부영 (서울시립대학교 환경원예학과) ;
  • 오성덕 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 권순종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서석철 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 류태훈 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 김경환 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박종석 (농촌진흥청 국립농업과학원 농업생명자원부)
  • Received : 2011.10.25
  • Accepted : 2011.11.07
  • Published : 2011.12.31
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Abstract

BACKGROUND: Soybean [Glycine max (L.) Merrill] is a legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn't show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified ${\gamma}$-tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.

본 연구는 국내에서 개발된 형질전환 콩 재배 시 토양 미생물 군집에 미치는 영향과 수평적 유전자 이동 여부를 알아보기 위해 수행되었다. 성숙기 토양의 미생물 군집밀도의 경우 형질전환 콩 근권 토양 미생물 군집밀도가 비 형질전환 콩 근권 토양과 유사하여 형질전환 콩 재배가 근권 토양 미생물에 영향을 미치지 않는 것으로 나타났다. 근권 토양의 우점 미생물 분포 양상 분석 결과, Proteobacteria, Firmicutes와 Actinobacteria 순으로 나타났으며 점유율은 다소 차이를 보였으나 우점종은 거의 유사하였다. 근권 토양 DNA에 대한 DGGE 분석 결과, 형질전환 콩과 비 형질전환 콩의 근권 토양 미생물 군집의 변화는 보이지 않았다. 형질전환 콩 재배에 따른 토양의 화학성을 분석한 결과, 형질전환 콩과 비 형질전환 콩의 근권 미생물상의 명확한 차이가 나타날 정도로 토양간 화학성의 차이는 크지 않았다. 형질전환 작물에 도입된 유전자군을 대상으로 식물체와 근권 토양 DNA에 대한 PCR 분석을 수행한 결과 수평적 유전자 이동성은 일어나지 않은 것으로 추정되었다.

Keywords

References

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